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Addgene
Showing: 21 - 27 of 27 results
  1. bNS2B47NS3

    Plasmid
    #155320
    Purpose
    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length
    Depositor
    Insert
    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  2. OA984-HA

    Plasmid
    #120362
    Purpose
    Expresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporter
    Depositor
    Insert
    1C19 his-tagged single chain antibody
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    AAEL010782 carboxypeptidase A (CPA) gene Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. gNS2B47NS3 L30SF31S

    Plasmid
    #155316
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant
    Depositor
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Availability
    Academic Institutions and Nonprofits only
  4. eNS2B47NS3 L30SF31S

    Plasmid
    #155319
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant
    Depositor
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Availability
    Academic Institutions and Nonprofits only
  5. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Tags
    Expression
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pAG416GAL-DENV_NS3-GS-NS2B

    Plasmid
    #201940
    Purpose
    Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker
    Depositor
    Insert
    DENV NS3-GS-NS2B (POLY )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 27 of 27 results