Addgene

Showing: 1 - 20 of 80 results

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-DroshaK382R

Plasmid

#62519

Purpose

Lysine 382 of Drosha was mutated to arginine

Depositor

Bharat Ramratnam

Article

Tang et al PLoS One. 2013 Aug 29;8(8):e72503. doi:

Insert

human Drosha with lysine 382 changed to arginine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed lysine 383 to arginine

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300A

Plasmid

#62526

Purpose

Serine 300 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to alanine

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302A

Plasmid

#62527

Purpose

Serine 302 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to alanine

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300E

Plasmid

#62528

Purpose

Serine 300 of Drosha is mutated to glutamic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302D

Plasmid

#62529

Purpose

Serine 302 of Drosha is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to aspartic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to aspartic acid

Promoter

Availability

Academic Institutions and Nonprofits only
pENTRdeltaGFPmirGE-DroshaT1.5.5

Plasmid

#61084

Purpose

pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Depositor

Patrick Salmon

Article

Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

Insert

deltaGFP

Use

RNAi

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-sgRNA_Drosha_1

Plasmid

#79860

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_2

Plasmid

#79861

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
p3xFLAG-zDrosha

Plasmid

#171540

Purpose

Encodes Zebrafish Drosha protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

drosha (rnasen Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300E/S302D

Plasmid

#62531

Purpose

Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid and serine 3…

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374delNLS2

Plasmid

#62532

Purpose

N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

truncated human Drosha mutant (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269 and nuclear localizatio…

Promoter

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 80 results

Results per page:

GFP-Drosha

GFP-DroshaK382R

GFP-DroshaS300A

GFP-DroshaS302A

GFP-DroshaS300E

GFP-DroshaS302D

pENTRdeltaGFPmirGE-DroshaT1.5.5

pcDNA4/TO/cmycDrosha

pX330-sgRNA_Drosha_1

pX330-sgRNA_Drosha_2

pX330-sgRNA_Drosha_3

pX330-sgRNA_Drosha_4

p3xFLAG-zDrosha

GFP-Drosha1-390

GFP-Drosha1-850

GFP-Drosha218-1374

GFP-Drosha270-1374

GFP-DroshaS300/302A

GFP-DroshaS300E/S302D

GFP-Drosha270-1374delNLS2

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