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Showing: 21 - 40 of 67 results

  5. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid
    #114009
    Purpose
    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection
    Depositor
    Pavel Volchkov
    Article
    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126
    Inserts
    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    -, CMV, and endogenous promoter (chicken GAPDH in…
    Availability
    Academic Institutions and Nonprofits only
    114009-plasmid-map-sequence-id-261238

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  10. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Jackie Collier
    Insert
    shble
    Use
    Thraustochytrid expression
    Tags
    Expression
    Mutation
    Promoter
    GAPDH
    Availability
    Academic Institutions and Nonprofits only
    117226-plasmid-map-sequence-id-224760

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  11. SZ_EC_02

    Plasmid
    #155341
    Purpose
    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
    Depositor
    Jens Nielsen
    Article
    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:
    Inserts
    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    Use
    Easycloneyali insert vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    gapdh, pyk1, and tef1
    Availability
    Academic Institutions and Nonprofits only
    155341-plasmid-map-sequence-id-316921

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  13. pSETT4gap

    Plasmid
    #153413
    Purpose
    φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette
    Depositor
    Joern Kalinowski
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    gapDH promoter from Eggerthella lenta
    Availability
    Academic Institutions and Nonprofits only
    153413-plasmid-map-sequence-id-305128

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  14. pGAPTrap-Clover-Muro

    Plasmid
    #82334
    Purpose
    Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    Clover-T2AMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82334-plasmid-map-sequence-id-156570

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  16. pGAPTrap-IRESMuro

    Plasmid
    #82336
    Purpose
    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82336-plasmid-map-sequence-id-155824

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  17. pGAPTrap-mtagtdTom_IRESMuro

    Plasmid
    #82355
    Purpose
    Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    mtagTandemTomato-IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82355-plasmid-map-sequence-id-156569

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  18. pGAPTrap-eGFP-IRESMeo

    Plasmid
    #82502
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMeo
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82502-plasmid-map-sequence-id-156575

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  19. pGAPTrap-eGFP-IRESMygro

    Plasmid
    #82503
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMygro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82503-plasmid-map-sequence-id-161768

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  20. pGAPTrap-eGFP-IRESMuro

    Plasmid
    #82504
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82504-plasmid-map-sequence-id-156573

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Showing: 21 - 40 of 67 results