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Showing: 1 - 18 of 18 results

  4. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

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  5. psd44-G13GA

    Plasmid
    #46827
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    G225A
    Promoter
    PGK promoter
    Availability
    Academic Institutions and Nonprofits only

  6. psd44-G13WT

    Plasmid
    #46829
    Purpose
    expression of wild type G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Availability
    Academic Institutions and Nonprofits only

  8. psd44-G13QL

    Plasmid
    #46828
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q226L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Availability
    Academic Institutions and Nonprofits only
    46828-plasmid-map-sequence-id-137240

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  11. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha13-RLuc8
    GGamma9-GFP2
    GBeta3
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    196062-plasmid-map-sequence-id-385881

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Showing: 1 - 18 of 18 results