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Addgene
Showing: 1 - 20 of 133 results

  1. iLOV-C1

    Plasmid
    #54591
    Purpose
    Localization: C1 Cloning Vector, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. iLOV-N1

    Plasmid
    #54673
    Purpose
    Localization: N1 Cloning Vector, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63723-plasmid-map-sequence-id-110780

    Enlarge

  7. iLOV-Lifeact-7

    Plasmid
    #54746
    Purpose
    Localization: Actin, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. iLOV-Farnesyl-5

    Plasmid
    #56516
    Purpose
    Localization: Membrane, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. iLOV-Mito-7

    Plasmid
    #56519
    Purpose
    Localization: Mitochondria, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Use
    Tags
    iLOV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pET28-iLOV-Rsn2

    Plasmid
    #61687
    Purpose
    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV
    Depositor
    Brian Smith
    Insert
    Ranaspumin-2
    Use
    Tags
    His and iLOV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    61687-plasmid-map-sequence-id-259998

    Enlarge

  11. pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47449
    Purpose
    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NN_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47449-plasmid-map-sequence-id-72979

    Enlarge

  12. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47450
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5HD_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47450-plasmid-map-sequence-id-69442

    Enlarge

  13. pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47451
    Purpose
    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NG_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47451-plasmid-map-sequence-id-72980

    Enlarge

  14. pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47452
    Purpose
    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NI_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47452-plasmid-map-sequence-id-72981

    Enlarge

  15. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47454
    Purpose
    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-SID4X
    Use
    AAV
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Availability
    Academic Institutions and Nonprofits only
    47454-plasmid-map-sequence-id-69624

    Enlarge

  16. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

    Enlarge

Showing: 1 - 20 of 133 results