Addgene

Showing: 61 - 80 of 92 results

Results per page:

pAMP-CY1_NF1 T1 R1276P (+minintr, KDR)

Plasmid

#119202

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Tags

Expression

Mutation

R1276P

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAMP-CY1_NF1 T2 R1276P (+minintr, KDR)

Plasmid

#119203

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Tags

Expression

Mutation

R1276P

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

Plasmid

#156414

Purpose

kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

mutant KRAS4B-G12V

Promoter

kdrl

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KDR-COMP5AP-AviTag-9xHis

Plasmid

#157531

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KDR (KDR Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KDR-Fc(DAPA)-AviTag-6xHis

Plasmid

#156967

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KDR (KDR Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

Plasmid

#156415

Purpose

kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Zebrafish transgenesis

Tags

mTagBFP2

Expression

Mutation

mutant KRAS4A-G12D

Promoter

kdrl

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

Plasmid

#156416

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

kdrl

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

Plasmid

#180468

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

KRAS4A (KRAS Human)

Use

Zebrafish transgenesis

Tags

V5-mScarlet-I

Expression

Mutation

Promoter

kdrl

Availability

Academic Institutions and Nonprofits only

Enlarge
VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Use

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
VEGFR2-mCh

Plasmid

#108854

Purpose

Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Article

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Use

Tags

Labeled with mCherry on the C-Terminus via a 3 am…

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-VEGFR2-mCherry

Plasmid

#139606

Purpose

Expresses a VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXSN-FLK1 TM

Plasmid

#65249

Purpose

Expression of dominant negative FLK1 in mammalian cells

Depositor

Axel Ullrich

Article

Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

Insert

FLK1 TM (Kdr Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

deleted AA 786-1346

Promoter

LTR

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Y951F-VEGFR2-mCherry

Plasmid

#139607

Purpose

Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Y1175F-VEGFR2-mCherry

Plasmid

#139608

Purpose

Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1995

Plasmid

#144376

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KDR (KDR Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-VEGFR2-N

Plasmid

#54298

Purpose

Localization: Membrane Receptor, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

VEGFR2 (KDR Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pGL3-VEGFR2-780

Plasmid

#21307

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-VEGFR2-570

Plasmid

#21309

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only