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Addgene
Showing: 1 - 20 of 216 results

  4. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  8. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

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  20. mApple-ER-5

    Plasmid
    #54895
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 216 results