Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Showing: 1 - 20 of 207 results

  5. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Roger Tsien, Erik Rodriguez
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

    Enlarge

  6. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

    Enlarge

  16. mApple-ER-5

    Plasmid
    #54895
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  20. mApple-Display-5

    Plasmid
    #54543
    Purpose
    Localization: Cytoplasm, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mApple-Display
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 207 results