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Addgene
Showing: 121 - 140 of 215 results

  1. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179435
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Availability
    Academic Institutions and Nonprofits only
    179435-plasmid-map-sequence-id-355551

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  6. pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179433
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Availability
    Academic Institutions and Nonprofits only
    179433-plasmid-map-sequence-id-362558

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  8. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179437
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Availability
    Academic Institutions and Nonprofits only
    179437-plasmid-map-sequence-id-355553

    Enlarge

  10. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

    Enlarge

  11. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176627
    Purpose
    TF B self-activation cassette in MultiFate-2.2 and MultiFate-3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    176627-plasmid-map-sequence-id-347070

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  12. GTU-y

    Plasmid
    #81095
    Purpose
    Gene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    mCitrine
    Use
    Cre/Lox
    Tags
    mCitrine
    Expression
    Yeast
    Mutation
    Mutated mCitrine for monomeric isoform (A206K L22…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    81095-plasmid-map-sequence-id-154254

    Enlarge

  13. GTL-y

    Plasmid
    #81100
    Purpose
    Gene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    mCitrine
    Use
    Cre/Lox
    Tags
    mCitrine
    Expression
    Yeast
    Mutation
    Mutated mCitrine for monomeric isoform (A206K L22…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    81100-plasmid-map-sequence-id-154279

    Enlarge

  14. GTH-y

    Plasmid
    #81105
    Purpose
    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    mCitrine
    Use
    Cre/Lox
    Tags
    mCitrine
    Expression
    Yeast
    Mutation
    Mutated mCitrine for monomeric isoform (A206K L22…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    81105-plasmid-map-sequence-id-154304

    Enlarge

  15. GTT-y

    Plasmid
    #81110
    Purpose
    Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    mCitrine
    Use
    Cre/Lox
    Tags
    mCitrine
    Expression
    Yeast
    Mutation
    Mutated mCitrine for monomeric isoform (A206K L22…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    81110-plasmid-map-sequence-id-154338

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  20. pOCC230

    Plasmid
    #118871
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mCitrine, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Availability
    Academic Institutions and Nonprofits only
    118871-plasmid-map-sequence-id-232332

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Showing: 121 - 140 of 215 results