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Plasmid#110060PurposeTo visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)DepositorInsertmCherry-GFP-LC3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mCherry-EGFP-LC3B
Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B G120A
Plasmid#123235PurposeExpresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B
Plasmid#223712PurposeExpresses autophagy flux LC3B fluorescence reporterDepositorInsertLC3B (MAP1LC3B Human, Synthetic)
UseLentiviral and Synthetic BiologyTagsmCherry::EGFP::LC3BExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP-SNX9
Plasmid#170551PurposeTo monitor the turnover of SNX9 in lysosomesDepositorInsertSNX9 (SNX9 Human)
UseRetroviralTagsSNX9 in tandem with mCherry and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-Tandem-mCherry-EGFP
Plasmid#123216PurposeN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mC-G-LC3B-P
Plasmid#124974Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
AV0-BWS-2.0-mC-G-CL3B
Plasmid#124975Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only