Showing: 1 - 7 of 7 results
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Plasmid#209283PurposeExpresses Voltron1 and jGCaMP7f in the zebrafish heartDepositorInsertVoltron1_P2A_jGCaMP7f
UseZebrafish expressionTagsExpressionMutationPromoterzACNE20_myl7AvailabilityAcademic Institutions and Nonprofits only -
pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2
Plasmid#209282PurposeExpresses FR-GECO1c and CoChR in the zebrafish heartDepositorInsertFR-GECO1c_P2A_CoChR_KGC_eGFP_ER2
UseZebrafish expressionTagsExpressionMutationPromoterzACNE20_myl7AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-46: MYL7-mEGFP
Plasmid#114413PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertMYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pISceI-myl7:MBIC5-mCer3
Plasmid#74117PurposeZebrafish transgenic constructDepositorInsertMBIC5-mCer3
UseMrna generation for microinjection.TagsmCerulean 3ExpressionMammalianMutationPromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-NLS-BirA-2A-mCherry_Ras
Plasmid#80061PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2+ MBIC5-mCer3
Plasmid#74116PurposeFor generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)DepositorInsertMBIC5-mCer3
UseTransgenic constructTagsmCerulean 3ExpressionMutationPromoterMyl7 (Cmlc2)AvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 7 of 7 results