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Addgene
Showing: 1 - 20 of 260 results
  1. PPIC

    Plasmid
    #25606
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PPIC (PPIC Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pPICZ:mTREK-1_cryst

    Plasmid
    #133269
    Purpose
    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP
    Depositor
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Use
    Tags
    SNS- 3C_cleavage_site-TAAA-GFP
    Expression
    Yeast
    Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pPICZaa-STE2-cohis

    Plasmid
    #133433
    Purpose
    Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S/C252A, Codon optimized for P. pastoris, and …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pPICZac-STE2-1D4

    Plasmid
    #133437
    Purpose
    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S/C252A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Availability
    Academic Institutions and Nonprofits only
  7. FUGW-APPIce-T2A-mCherry

    Plasmid
    #190806
    Purpose
    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Icelandic (A673T)
    Promoter
    Ubi C
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 260 results