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pETDUET-GST AO7RE ∆238-245

Plasmid

#139318

Purpose

GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.RFPL4b.Luciferase.WPRE

Plasmid

#69252

Purpose

Luciferase in lentivirus backbone to report Dux4 activation of RFPL4b promoter

Depositor

Stephen Tapscott

Insert

RFPL4b (RFPL4B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RFPL4b

Availability

Academic Institutions and Nonprofits only

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pGL3 RFPL4b promoter

Plasmid

#69250

Purpose

Luciferase reporter for Dux4 activation of RFPL4b transcription

Depositor

Stephen Tapscott

Insert

RFPL4b (RFPL4B Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

RFPL4b

Availability

Academic Institutions and Nonprofits only
6CVZ (RFWD3)

Plasmid

#119964

Purpose

Baculovirus expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

RFWD3 (RFWD3 Human)

Use

Baculovirus expression

Tags

6xHis-TEV

Expression

Mutation

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRIM55_p.E369G

Plasmid

#81466

Purpose

Gateway Donor vector containing TRIM55 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRIM55 (TRIM55 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E369G

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRIM55_p.R322H

Plasmid

#81609

Purpose

Gateway Donor vector containing TRIM55 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRIM55 (TRIM55 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R322H

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRIM55_WT

Plasmid

#81829

Purpose

Gateway Donor vector containing TRIM55 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRIM55 (TRIM55 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_RFWD3:1-774

Plasmid

#210954

Purpose

Mammalian expression of full-length human RFWD3. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_RFWD3:1-774

Plasmid

#210955

Purpose

Mammalian expression of full-length human RFWD3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RFWD3:1-774

Plasmid

#211103

Purpose

Mammalian expression of full-length human RFWD3. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_RFWD3:1-774

Plasmid

#211108

Purpose

Mammalian expression of full-length human RFWD3. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:M1-E774 (RFWD3 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_COP1:1-731

Plasmid

#211156

Purpose

Mammalian expression of full-length human COP1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

COP1:M1-V713 (COP1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_RFWD3:425-774

Plasmid

#210884

Purpose

Insect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RFWD3:S425-E774 (RFWD3 Human)

Use

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_COP1:386-731

Plasmid

#210887

Purpose

Insect expression of human COP1 fragment A386 to V731. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

COP1:A386-V731 (COP1 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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