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Addgene
Showing: 61 - 80 of 6957 results

  1. pTSara-R756S

    Plasmid
    #60725
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60725-plasmid-map-sequence-id-97713

    Enlarge

  2. pTSara-N748D

    Plasmid
    #60726
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60726-plasmid-map-sequence-id-97714

    Enlarge

  3. pTSara-R756K

    Plasmid
    #60727
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60727-plasmid-map-sequence-id-97715

    Enlarge

  5. pDONR221-SAR1

    Plasmid
    #67344
    Purpose
    hSar1 with stop codon in Gateway Entry vector
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only

  6. pDONR221-SARA

    Plasmid
    #67346
    Purpose
    hSara with stop codon in Gateway Entry vector
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only

  7. pDEST14-SAR1

    Plasmid
    #67388
    Purpose
    Expression of native hSar1 in E. coli
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  8. pDEST14-SARA

    Plasmid
    #67389
    Purpose
    Expression of native hSara in E. coli
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  9. pDEST47-SAR1

    Plasmid
    #67472
    Purpose
    Mammalian expression of hSar1 with stop codon (native expression)
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  10. pDEST47-SARA

    Plasmid
    #67473
    Purpose
    Mammalian expression of hSara with stop codon (native expression)
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  11. 5JJX (SART3)

    Plasmid
    #101879
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SART3 (SART3 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101879-plasmid-map-sequence-id-194706

    Enlarge

  12. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    101880-plasmid-map-sequence-id-194708

    Enlarge

  13. pCAGGS-ROSAr

    Plasmid
    #54324
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGG
    Availability
    Academic Institutions and Nonprofits only
    54324-plasmid-map-sequence-id-124429

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Showing: 61 - 80 of 6957 results