We narrowed to 1,015 results for: LEA

...Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger Consortium: Nuclease Expression Vectors This...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created...cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian ...in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric...

...is would change over the next twenty years. But learn we did! From Addgene's very beginning, we had to...information about images that a sighted reader would glean from the image itself.  As we incorporated alt text...to alt text. We also were aware of the need for clear, accessible writing in scientific writing, and how...development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.” In this post... of writing: alt text (pun intended.)  Steps to clear writing Let’s use Figure 1 as our example. Remember...student using viral vectors for the first time and learning how to purify AAV.  Second, you’ll identify your...free to share your alt text in the comments! We learned a lot from listening to others' alt text descriptions...

...MEDLINE-indexed journal Nuclear Receptor Signaling. Dr. McKenna edits the website of the Nuclear Receptor Signaling... elicit their actions on other tissues. At the Nuclear Receptor Signaling Atlas (NURSA), one of our primary...regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators, and their ...their physiological endocrine ligands. Nuclear receptors and their ligands NRs represent the largest family...Consortium represents a unique funding model in nuclear receptor research, and has generated a set of comprehensive...address many important and fundamental questions in nuclear receptor and coregulator research. Our Q-PCR datasets...for example, document the expression patterns of nuclear receptors across multiple different tissues in ...

...continuing the project after leaving the meeting. Follow the tips below to learn how you can run a top-notch...direction you’d like to pursue? Undefined scope can lead to long meetings spent on details that do not yet...our all-company meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting...screen using a Google Doc. Action items: Before leaving the meeting, skim back through your notes and pick... tend to get stuck diving into details, ask a colleague to help keep you accountable for moving along....your organization. Ask for feedback from your colleagues. When you attend a well-run meeting, note what...what worked well and ask the meeting leader for some tips. Before long, you will be running collaborative...

... Connie Cepko to learn about her management style. Leaders look to the future Leaders must get new things... they lead. Effective leaders rarely give orders (although they must occasionally). Good leaders exert...urged you to work on developing your “leadership skills”. Leadership is prized at all levels of an organization...figure out what skills were really leader-specific. How is leadership different from good management? Aren't...Manager, Leader” by Charlotte Beers, divides workers into three cycles: Doers, Managers and Leaders. I like... Mask: How Leaders Spark and Sustain Change, by Peter Fuda and Richard Badham Leadership Insights for...Business Review 10 Must Reads: On Leadership (Articles on Leadership, also can be purchased individually...

...wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally susceptible...transient leaf transformation, the engineered Agrobacterium strain is brought into contact with the leaf tissue...Agrobacterium infiltration into leaves does not work well, as the leaves have a waxy texture and are difficult...transgenic plant can take even longer, a transient plant leaf transformation process could save the plant biologist...studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in roots. The development...development of transgenic hairy roots takes at least six months. Generating a transgenic plant takes even...biosynthetic pathway. Why plant scientists do transient leaf transformations The development of transgenic plants...

...opportunities to learn from other biotech companies at workshops and can use what we learn to improve processes... end repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to... post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop...CosMCPrep system for this, but thought we could learn a little bit more about high throughput DNA prepping...class at NEB’s new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space...emerging biotech companies. The space was modern with clean bold colors of orange and white. The decor was very... this is the most important part of this newly released prep kit. In their prior NEBNext Ultra II DNA ...

... led for 5 years.  Different leadership styles Being an effective leader requires you to understand how...would come necessary changes. I also learnt to adapt my leadership style according to the needs of my committee...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need ...that come with leadership. The NPA was a great organization to develop these leadership skills in particular... These “leadership institute’ weekends would entail intense training to develop our leadership and management...sharing with other more junior leaders what I had experienced and learnt. Opening doors to further opportunities...develop my leadership and advocacy skills but will also put me in closer connection with other leaders in my...

...Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...for Leaders More resources on the Addgene blog Management for Scientists: Managing vs. Leading Management...to a managerial role. For those contemplating the leap into management, the journey can be as exciting ...intended) expectations —yours and your team's—can lead to frustration. Clinging to technical tasksDelegation... member has unique motivations, strengths, and learning styles. For example, during my time as a lab scientist...required more guidance and structure. I quickly learned that my approach needed to adapt based on individual...individual needs. For instance, I had one colleague who preferred detailed instructions and regular check-ins...

...Considerations when choosing your Cas nuclease When choosing the Cas9 nuclease for your experiments, there are...but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this...determined by the PAM sequence recognized by the nuclease. For the most frequently used Cas9, spCas9 from... Figure 1: The PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s...off-target activity of Cas9. This can occur when Cas9 cleaves a site that is not a perfect match to the guide...difficult to determine, the potential for off-target cleavage should be an important consideration during any... measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel...

...from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of PRC2 with RvLEAMshort (1:6 molar ratio... imaging. A truncated form of a tardigrade LEA, RvLEAMshort, protected samples and was compatible with...tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim, C. J. (2024). Small LEA proteins as an effective...MagIC-Cryo-EM for enrichment and structure determination LEA proteins protect samples during cryo-EM grid freezing...: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid...lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that naturally adsorb to...under CC-BY-NC-ND 4.0 license. Protection by the LEAs allowed the authors to determine structures at comparable...

...neurons (measured using a red calcium sensor) leads to the release of serotonin (measured using the newly developed...shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence can interfere...explains. Developing an artificial leaf replica means creating a “leaf” with similar permeability of water...for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach plans to use a suite of fluorescent... determine whether the artificial leaf behaves like the living leave in this aspect. To monitor bacterial... the variant is unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing ...dosages.  Michal Bernach: Developing an artificial leaf replica system to study plant-bacteria and bacteria-bacteria...

...this tour of Addgene’s new conference rooms and learned some fun facts about these genes and the origins...

...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating...binding. In this formation, its nuclease domains, RuvC and HNH, cleave opposite strands of the target ...popular Cas endonuclease, with many modified versions engineered to increase specificity and nuclease activity...Cas9 The two Cas9 nuclease domains, RuvC and HNH, can be altered to reduce cleavage events to a single...contains mutations in both nuclease domains, D10A and H840A, resulting in no cleavage of target DNA. Cas9n ...increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity...

...HLALS NFYA nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,... growth hormone releasing hormone GHRF, GRF, MGC119781 GHRHR growth hormone releasing hormone receptor... MGC126867 GNRH1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) GNRH, GRH, LHRH, LNRH...GNRH2 gonadotropin-releasing hormone 2 GnRH-II, LH-RHII GNRHR gonadotropin-releasing hormone receptor GNRHR1...antimicrobial peptide HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR HBEGF heparin-binding EGF-like growth ...NROB1 NR0B2 nuclear receptor subfamily 0, group B, member 2 FLJ17090, SHP, SHP1 NR1D1 nuclear receptor subfamily...

...also promotes non-specific RNA cleavage, but this non-specific cleavage does not occur in mammalian cells...genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune ...Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which...The CRISPR complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure ...the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA...Cas9 separates the double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA... on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was the first system...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...viral particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...

...tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC...with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC...with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108...pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and ...pFBOH-LIC EF456740 Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117...pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector...

...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli E... E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease VIII 165571 pOpen-Exonuclease...pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4...Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-...

...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...page for more information. To learn more about Addgene's MTA process please visit our Help Center . The ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to order...Addgene as well as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer...

...Expression mCerulean 433 475 27 4.7 Monomer (A206K) Cerulean - Mammalian Expression pCS2+8CCerulean - Zebrafish...pCS2+8NCerulean - Zebrafish/Xenopus/Worm/Sea urchin mCerulean N1 - Mammalian Expression mCerulean C1 -...Expression mCerulean3 433 475 32 3.2 Monomer (A206K) mCerulean3-N1 - Mammalian Expression mCerulean3-C1 - Mammalian... Mammalian Expression moxCerulean3 434 474 36 3.2 Monomer (A206K) moxCerulean3 - Mammalian Expression ...different colors as needed for your experiments. Learn more about the flexibility of self-labeling tags...

...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...also important to consider factors that maximize cleavage of the desired target sequence, or on-target activity...exhibits the lowest immunogenicity and are one of the least toxic methods for in vivo viral delivery. Additionally...Before proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with...Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before...

...a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer...sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the...these tools and the researchers who are learning to wield them. Learn more about Addgene’s TAL effector and...years of research has shown that when fused to a nuclease or activation domain, a ZF array is an effective...at Massachusetts General Hospital also recently released a TALEN kit through Addgene. This kit, first described...

...available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon Boye and ...AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...

...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...

...pmEYFP-1 , pECFP18aaEYFP mCerulean mVenus 433 0.49 527 104,000 0.64 5.3 2.3 mCerulean N1 , mVenus N1 mTurquoise2...Koushik et al. (2006) Cerulean, Venus, and VenusY67C FRET reference standards mCerulean-mVenus FRET efficiency...referred to as intramolecular or unimolecular FRET). Learn more about FRET on our blog and explore the tables...proteins connected via flexible peptide linkers mCerulean, mVenus, and Amber (mVenus Y67C) FRET standards...its use for measuring resonance energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for...and Fixed Cells Additional Resources at Addgene Learn more on the Addgene Blog: Introduction to FRET and...

...MDH). The MCC is a national group of educational leaders providing research opportunities to their students...MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the 6xHis-tag and the ... region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available... CUREs Community (Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

...CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn...DREADDs Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825...GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Jonathan Ting 51083 AAV-...AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium sensor Jonathan ...GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Jonathan Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate genome...nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA. For most applications...gain-of-function screening. For more information, please visit the Addgene SAM library page . Targeted gene... directed repair-mediated Kras G12D mutations, leading to macroscopic tumors of adenocarcinoma pathology... DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann...

...detection in western blots, or removed using protease cleavage sites. Other protein tags and signal peptides ... for protein purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags...GST Solubility tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor Xa, enterokinase, ...Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET Wolf Frommer 65618... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...interest at high levels. Perhaps high expression leads to slow bacterial growth, or maybe you only want...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...