We narrowed to 392 results for: Lor

...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...plasmids in the repository. Browse the University of Florida serotype testing panel for the eye and brain. Viral...Viral Vector Packaging Service AAV University of Florida Serotype Testing Panel for the Eye and Brain Viral...Viral Vector Packaging Service: University of Florida Serotype Testing Panel for the Eye and Brain You...-use viral preparations from the University of Florida for enhanced transduction of the eye and other ...Srivastava’s or Shannon Boye’s lab at the University of Florida. References for each serotype can be found below...

... Work by Kamio et al. (2025) utilized HiBiT to explore TP53 biology. They first identified that in a set...most frequently mutated genes. Next, the authors explored how these variants of p53 shape chemosensitivity...internalization Work conducted internally at Promega explored the use of HiBiT to track epidermal growth factor...) utilized HiBiT to address these challenges, exploring the binding kinetics of the VEGF-A and the VEGFR2...

...way they generate their color: they strongly absorb visible light to give colors in ambient light. In contrast...fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name a few. What’s behind these sights... In 1987, scientists described an unusual blue-colored protein from the jellyfish Cassiopea xamachana ...used for mimicry and camouflage. An anemone’s red colored tentacles resemble worms which attract fish for...Figure 1). Figure 1: The sea anemone's red color can be used for mimicry. Image: Bernard Spragg. ...chromoproteins absorb visible light and give off color in ambient light, it gives scientists the ability... For each chromoprotein, the lab determined the color intensities, maturation times, and fitness cost ...

...introduced at ~2 copies per cell per color to achieve the highest color variations. Adjust the plasmid concentrations...Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for discriminating...neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP system to...with different copy numbers, producing dozens of color hues (Livet et al., 2007; Cai et al., 2013). However...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...genes is introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the ...Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent...

...least a couple of decades. Green is the classic color, but fluorescent proteins are available in a variety...iRFP720, as photoacoustic contrast agents for two-color imaging in animals. The approach relies on ultrasound....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...

...shown in the darker colors and the mCherry spectrum is shown in the lighter colors; excitation spectra...in choosing fluorescent proteins for your multi-color imaging experiment is to be aware of what fluorescent...fluorescent proteins Commonly used filter sets for multicolor imaging include ones designed for CFP, YFP, and...compartments. I hope this sheds some light on multicolor imaging with fluorescent proteins. With the right...right choice of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward... multi-color imaging...

...to a rainbow of colors. With three copies of the construct, one would expect ten colors (see table to the...distinct colors due to higher copy number. The name Brainbow is truly fitting for this colorful technology...and high stability of colors. Sanes and Lichtman estimate that Brainbow’s colorful labeling has decreased...will highlight the evolution of the  Brainbow multicolor labeling system - a perfect example of the continued... Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains...Cre-mediated excision removes one segment, eliminating two color possibilities. The remaining cassette can invert...the mouse brains, they found many more than 3-4 colors due to the tandem integration of multiple copies...

...variety of colors when a combination of XFP are expressed. Each cell remains the same color for its entire...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This...containing fluorescent proteins (XFP) of different colors to label an organ or entire organism. Because each...entire lifetime and daughter cells retain the same color, allowing for the fate mapping of cell populations...persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow, now...protein expressed in a single cell maintains its color over the entire length of the cell, even axons of...improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila neurons...

...Constitutive 1 Loren Looger 137955 pAAV.CAG.iAChSnFR CAG iAChSnFR none Constitutive 1, 9 Loren Looger 121922..., rg* Loren Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Loren Looger...Constitutive 1, 9 Loren Looger 179254 AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Loren Looger..., rg* Loren Looger 176755 AAV-mDlx-jGCaMP8s-WPRE Dlx jGCaMP8s none Constitutive 1, 9, rg* Loren Looger...Constitutive 1, 9 Loren Looger 179256 AAV-CAG-jGCaMP8s-WPRE CAG jGCaMP8s none Constitutive 1, 9, rg* Loren Looger..., rg* Loren Looger 176754 AAV-mDlx-jGCaMP8m-WPRE Dlx jGCaMP8m none Constitutive 1, 9, rg* Loren Looger... 5, 9 Loren Looger 176757 AAV-EF1a-jGCaMP8m-WPRE EF1a jGCaMP8m none Constitutive 1, 9, rg* Loren Looger...

...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...415-25. Martin Webb ATP Multicolor palette of ATP indicators (MaLion) RGB-color intensiometric indicators...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren Looger , GENIE Project Calcium GCaMP3 genetically...indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors Engineering ...

...Biosensor Loren Looger AV-1-PV2724 98931-AAV1 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-5-PV2724 98931-AAV5 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-9-PV2724 98931-AAV9 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren Looger ...AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40...AAV1 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV3080 100840-AAV1 pAAV.CAG.GCaMP6m....100832-AAV1 pAAV.hSyn.CaMPARI.WPRE.SV40 Biosensor Loren Looger, Eric Schreiter AV-1-PV3844 100855-AAV1 pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40...

... CAG GFP Control Loren Looger 116870 pAAV-CAG-H2B tdTomato CAG tdTomato Control Loren Looger 24593 AAV-pgk-Cre...Calcium sensor Loren Looger 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn H2B-GCaMP8s Calcium sensor Loren Looger 176750...Calcium sensor Loren Looger 176751 AAV-CamKIIa-jGCaMP8m-WPRE CamKII GCaMP8m Calcium sensor Loren Looger 176752...Calcium sensor Loren Looger 176753 AAV-mDlx-jGCaMP8f-WPRE Dlx GCaMP8f Calcium sensor Loren Looger 176755...Calcium sensor Loren Looger 176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium sensor Loren Looger 176757...Calcium sensor Loren Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium sensor Loren Looger 179254...Calcium sensor Loren Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m Calcium sensor Loren Looger 179256...

... Here, HRP is used as a colorimetric, in which the reaction produces a colored product that accumulates...well. In colorimetric assays, protein amount can be determined by correlating the amount of color in a well...small multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for...molecules , proteins that emit a measurable light or color in response to a specific stimulus, can be conjugated...than HRP, fluorophores are available in a range of colors activated by different wavelengths, allowing for...indirect or direct method. Since IF uses fluorophore color to differentiate between the targets of interest...theoretically allow for roughly fifty different fluorophore colors in flow cytometry, but the largest panels used ...

...MoChlo: Modular Cloning Chloroplast Toolbox Library of standardized chloroplast-specific genetic modules...Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene ...Golden-Gate cloning of synthetic operons into chloroplast transformation vectors. Golden Gate (MoClo) Scott...plasmids that feature fluorescent, luminescent, and color-producing proteins for detection of gene expression... and pictures of plants, roots, shoots, and inflorescences - a growing resource for the plant science...

...TALEN kit (Bogdanove/Voytas) in order to generate TALORs (TAL Orthongal Repressors) that can be used to ...to custom repress gene expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with...regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this vector under galactose-inducible...TEF1 promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate...Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use in labeling...

...Constitutive rg* Loren Looger 116870 pAAV-CAG-H2B tdTomato CAG H2B-tdTomato Constitutive rg* Loren Looger 105530...Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags... GFPsm_myc (does not fluoresce) Cre dependent 1 Loren Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...Ruby2sm-Flag (does not fluoresce) Cre dependent 1, 5 Loren Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP...

...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...you plan your experiment. Tables are organized by color of the fluorescent protein. The content for this... (Stokes shift), useful for applications like multicolor imaging with better spectral separation. Protein...you can label one fusion protein with different colors as needed for your experiments. Learn more about...on our blog . Protein Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination...Blog: Choosing Your Fluorescent Proteins for Multi-Color Imaging FPbase (Link opens in a new window) : an...

...Takeshi Imai Return to Top Other Tet Applications Explore some other popular applications of tetracycline...dox-inducible expression of GFP T2A Cre recombinase. Floris Foijer 165457 AAVS1-Neo-TRE-CMV-Cre-rtTA Dox-inducible...104112 TetBow Series of plasmids for stochastic multicolor labeling for discriminating between neurons. ...Hasan, M. T., Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent ...

...Additional Resources (Link opens in a new window) Explore cutting-edge research tools from Promega — now ... protein expression and interactions, such as exploring viral biology without disturbing the virus of ...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find...

...individual links to each gene page are provided below. Color is used for clarity and does not indicate a specific...Frederick National Laboratory for Cancer Research Color is used for clarity and does not indicate a specific...signaling pathways, mutations and their role in colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest...

...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available....without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and...

...fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal... L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis for mismatch surveillance...Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin, F., Prandi, D., Romanel, A., Demichelis, F., ...Zhang, H., Finkelstein, I. J., Johnson, K. A., & Taylor, D. W. (2024). Unraveling the mechanisms of PAMless...Wolfe, S. A., Zhang, S., & Pederson, T. (2015). Multicolor CRISPR labeling of chromosomal loci in human ...., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation...

...56 °C for 30 min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Microcentrifuge....X 10 cm tissue culture dish, Corning 430167 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...add 10 mL fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM...DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro...

...are also included: using Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in...Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol RNase-free...for precipitation step, Option A) 7.5 M Lithium Chloride (for precipitation step, Option B) Glycogen (Optional...phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously...(s). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA ...from the homogenized sample(s). Add 0.2 mL of Chloroform/Isoamyl alcohol (49:1) per 1 mL of TRIzol® used...

... add hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Heat-inactivated...Corning 430167 15 mL conical tubes, VWR 21008-216 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...mL conical and an appropriate amount of 25 mM chloroquine to the conical so that the final concentration...transfection mixes are added) will be 25 uM of chloroquine. After the incubation, add the DNA:PEI-Max mix...mix to the conical containing the media and chloroquine and mix well. Gently aspirate the media out of...

...photoconvertible, and photoswitchable proteins, organized by color and expression species. The New England Biolabs ...Riboswitch Cobalamin (vitamin B12) Escherichia coli Remy Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing...Luciferase Fluorescent Biosensors Chromoproteins: Colorful Proteins For Molecular Biology Experiments Return...

...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For...

...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these .... Each point represents a single cell, and each color represents the population obtained with one CRISPR...