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We narrowed to 379 results for: Lor

Showing: 1 - 30 of 379 results

  1. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...plasmids in the repository. Browse the University of Florida serotype testing panel for the eye and brain. Viral...Viral Vector Packaging Service AAV University of Florida Serotype Testing Panel for the Eye and Brain Viral...Viral Vector Packaging Service: University of Florida Serotype Testing Panel for the Eye and Brain You...-use viral preparations from the University of Florida for enhanced transduction of the eye and other ...Srivastava’s or Shannon Boye’s lab at the University of Florida. References for each serotype can be found below...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...introduced at ~2 copies per cell per color to achieve the highest color variations. Adjust the plasmid concentrations...Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for discriminating...neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP system to...with different copy numbers, producing dozens of color hues (Livet et al., 2007; Cai et al., 2013). However...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...genes is introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the ...Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...way they generate their color: they strongly absorb visible light to give colors in ambient light. In contrast...fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name a few. What’s behind these sights... In 1987, scientists described an unusual blue-colored protein from the jellyfish Cassiopea xamachana ...used for mimicry and camouflage. An anemone’s red colored tentacles resemble worms which attract fish for...Figure 1). Figure 1: The sea anemone's red color can be used for mimicry. Image: Bernard Spragg. ...chromoproteins absorb visible light and give off color in ambient light, it gives scientists the ability... For each chromoprotein, the lab determined the color intensities, maturation times, and fitness cost ...

  5. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...least a couple of decades. Green is the classic color, but fluorescent proteins are available in a variety...iRFP720, as photoacoustic contrast agents for two-color imaging in animals. The approach relies on ultrasound....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...

  6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...shown in the darker colors and the mCherry spectrum is shown in the lighter colors; excitation spectra...in choosing fluorescent proteins for your multi-color imaging experiment is to be aware of what fluorescent...fluorescent proteins Commonly used filter sets for multicolor imaging include ones designed for CFP, YFP, and...compartments. I hope this sheds some light on multicolor imaging with fluorescent proteins. With the right...right choice of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward... multi-color imaging...

  7. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...to a rainbow of colors. With three copies of the construct, one would expect ten colors (see table to the...distinct colors due to higher copy number. The name Brainbow is truly fitting for this colorful technology...and high stability of colors. Sanes and Lichtman estimate that Brainbow’s colorful labeling has decreased...will highlight the evolution of the  Brainbow multicolor labeling system - a perfect example of the continued... Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains...Cre-mediated excision removes one segment, eliminating two color possibilities. The remaining cassette can invert...the mouse brains, they found many more than 3-4 colors due to the tandem integration of multiple copies...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...variety of colors when a combination of XFP are expressed. Each cell remains the same color for its entire...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This...containing fluorescent proteins (XFP) of different colors to label an organ or entire organism. Because each...entire lifetime and daughter cells retain the same color, allowing for the fate mapping of cell populations...persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow, now...protein expressed in a single cell maintains its color over the entire length of the cell, even axons of...improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila neurons...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...415-25. Martin Webb ATP Multicolor palette of ATP indicators (MaLion) RGB-color intensiometric indicators...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren Looger , GENIE Project Calcium GCaMP3 genetically...indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors Engineering ...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Biosensor Loren Looger AV-1-PV2724 98931-AAV1 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-5-PV2724 98931-AAV5 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Biosensor Loren Looger AV-9-PV2724 98931-AAV9 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV...Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren Looger ...AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40...AAV1 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV3080 100840-AAV1 pAAV.CAG.GCaMP6m....100832-AAV1 pAAV.hSyn.CaMPARI.WPRE.SV40 Biosensor Loren Looger, Eric Schreiter AV-1-PV3844 100855-AAV1 pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40...

  11. Antibody Guide

    Type
    Collection
    ... Here, HRP is used as a colorimetric, in which the reaction produces a colored product that accumulates...well. In colorimetric assays, protein amount can be determined by correlating the amount of color in a well...small multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for...molecules , proteins that emit a measurable light or color in response to a specific stimulus, can be conjugated...than HRP, fluorophores are available in a range of colors activated by different wavelengths, allowing for...indirect or direct method. Since IF uses fluorophore color to differentiate between the targets of interest...theoretically allow for roughly fifty different fluorophore colors in flow cytometry, but the largest panels used ...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Should I Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent...non-GFP proteins that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid...with your gene of interest. These are organized By Color of the fluorescent protein and By Expression Species...Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation and Emission maximum...lentiviral expression Verkhusha Lab Plasmids - Enhanced colors, including PSmOrange, iRFP Gradia Lab Mammalian...Vectors - Includes tagging with a wide variety of colors, some specifically optimized for yeast Lindquist...557-62 Olympus Confocal : The Fluorescent Protein Color Palette NIC Wiki : Nikon Imaging Center at UCSF ...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...TALEN kit (Bogdanove/Voytas) in order to generate TALORs (TAL Orthongal Repressors) that can be used to ...to custom repress gene expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with...regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this vector under galactose-inducible...TEF1 promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate...Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use in labeling...

  14. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core...Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral and...Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of Medicine Gene Vector Core (Link opens...of a variety of promoters. Professor Oka and the Baylor College of Medicine Gene Vector Core have also ...

  15. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... effective brightness and allow for single or multicolor imaging. Photo-activatable iRFPs can be ‘turned...vivo imaging tools in this blog post - In Living Color: The Skinny on In Vivo Imaging Tools . The following...empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging...

  16. Plant Plasmids and Resources

    Type
    Collection
    ... Modular Cloning Chloroplast Toolbox for design of synthetic operons for chloroplast transformation and... and pictures of plants, roots, shoots, and inflorescences - a growing resource for the plant science...

  17. Ras Pathway

    Type
    Collection
    ...individual links to each gene page are provided below. Color is used for clarity and does not indicate a specific...Frederick National Laboratory for Cancer Research Color is used for clarity and does not indicate a specific...signaling pathways, mutations and their role in colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest...

  18. CRISPR Guide

    Type
    Collection
    ...fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal... L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis for mismatch surveillance...Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin, F., Prandi, D., Romanel, A., Demichelis, F., ...Zhang, H., Finkelstein, I. J., Johnson, K. A., & Taylor, D. W. (2024). Unraveling the mechanisms of PAMless...Wolfe, S. A., Zhang, S., & Pederson, T. (2015). Multicolor CRISPR labeling of chromosomal loci in human ...., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL : A multicolor atlas for whole-brain neuronal identification...scFvs . Niwa et al. Cytoskeleton. 2023 Apr 10. Explore the recombinant monoclonal antibodies and scFvs...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline...104112 TetBow Series of plasmids for stochastic multicolor labeling for discriminating between neurons. ...Hasan, M. T., Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent ...

  21. Fluorescent Protein Guide

    Type
    Collection
    ...Should I Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent...and Digital Imaging Olympus Fluorescent Protein Color Palette...

  22. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For...

  23. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these .... Each point represents a single cell, and each color represents the population obtained with one CRISPR...

  24. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources and protocols...

  25. mTOR Pathway

    Type
    Collection
    ...individual links to each gene page are provided below. Color is used for clarity and does not indicate a specific... with the help of David Sabatini . mTOR Pathway Color is used for clarity and does not indicate a specific...

  26. Microbiology Resources

    Type
    Collection
    ...Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria...plasmid collection is organized by application and by color. External Resources European Saccharomyces Cerevisiae...

  27. p53 Pathway

    Type
    Collection
    ...below. Color is used for clarity and does not indicate a specific relationship. p53 Pathway Color is used...

  28. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...translational technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human ...

  29. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available....without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and...

  30. General Transfection

    Type
    Protocol
    ...56 °C for 30 min. 25 mM chloroquine diphosphate Dissolve 0.129 g of chloroquine diphosphate salt into 10...slowly add hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid ... Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear MW 25,000 Da Microcentrifuge....X 10 cm tissue culture dish, Corning 430167 Hydrochloric acid Sodium hydroxide 0.22 μm polyethersulfone... will be basic and will need adjustment with hydrochloric acid first. Pro-Tip The pH of this solution ...add 10 mL fresh DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM...DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro...
Showing: 1 - 30 of 379 results