...Letter Code Codons (RNA) Alanine Ala A GCU, GCC, GCA, GCG Arginine Arg R CGU, CGC, CGA, CGG, AGA, AGG ...

...requests faster than jGCaMP7 (Figure 5B).   Figure 5: A) GCaMP6, jGCaMP7, and jGCaMP8 viral preps distributed...Figure 5A). We then added jGCaMP7 and eventually jGCaMP8, later generations of GCaMP6, to the service. While...) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year from 2016–2023. All three...generations: jGCaMP8 allows for the “picture” of a train of action potentials that GCaMP6 and jGCaMP7 do not... for instance, the GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator...they were both requested, GCaMP6 remains the more requested option, and jGCaMP8 appears to be growing in...be a matter of timing, as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of the...

...-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest...pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c variant...potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f variant...potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all our biosensors inventory, and check...Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg) pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics...sensors The latest calcium sensors from Janelia (jGCaMP7) continue to be useful for monitoring neuronal ...

...There are three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but...zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors are also suitable for in vivo...slower). GCaMPf may be suitable for very rapid measurements, like action potentials in neurons. jGCaMP7 and...localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif...axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and...to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters...and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...

... add TAAGCA, resulting in a final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. ... stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then TAAGCA to improve restriction...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium...inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.F102G...calcium binding. There are a few versions of these jGCaMP7 sensors with different properties (for example,...applications. We recently released a new variant of jGCaMP7 (keep reading) and will be releasing more variants...like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV ...Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity...Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between...

...Flp-dependent GCaMP, AAV8-105714. Several new options are also available for the calcium sensor jGCaMP7 from Janelia... Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most ...104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate...

...axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors...sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)   There are hundreds...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...

...  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262...AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010  AAV9... pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics ...AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic...Among other new biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  ...

...repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes, as well as seven...Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-WPRE  AAV9 Biosensor...serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor Fyhn...

... Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1. The GCaMP8 viral vectors from...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus... have arrived! We now offer several versions of GCaMP8 vectors in serotypes AAV1 and AAV5. AAV9 preps ...viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in ...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing...

...viral tool AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute...viral tool AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA PHPeB Biosensor The Allen Institute...Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE AAV5 Biosensor GENIE Project New serotype...

...optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...of T5 neurons by placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically...

...AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor...

...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ...cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...

... add TAAGCA, resulting in a final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. ... stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then TAAGCA to improve restriction...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...

...biosensor, GCaMP, and click through to the plasmid search results: More than 200 different GCaMP containing...

...sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are optimized for excitation wavelengths ...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...

...Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE PHP.eB Biosensors GENIE Project New serotype...

...Optogenetics Karl Deisseroth New serotype AAV-CamKIIa-jGCaMP8s-WPRE AAV5 Biosensors Loren Looger New serotype...

...viral preps from recent months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection...

...parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...

... sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck...

...you use for expression of AAV cargo? Did you use GCaMP expression? Direct Fluorescence? Immunohistochemistry...

... Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2  Additional resources on the Addgene...

...methyl group to the adenine in AACNNNNNNGTGC or GCACNNNNNNGTT Impact on cloning and digests Although not ...

...completed, Hubbard will image these neurons using GCaMP7, measuring calcium signaling related fluorescence...

...and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur...

... - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors...

...expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are interested in...

... 5’ CCGG AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo: 5’...AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO...

...adam15 TAL3016 & TAL3017 TGTTTCTGGCGGACTCTCgtcaccattcttctgtTTGAAGGCACACCTTTGA ahcyl1 TAL3018 & TAL3019...TAL3019 TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA amh TAL3210 & TAL3211 TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA...TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA arf6 TAL3212 & TAL3213 TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA arnt1 TAL3214 & TAL3215 TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA... TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA...arap3a TAL3026 & TAL3027 TGGCAACAATCGGTCCGTgctcagatgcagaggaCCTGTTGGCTTCCATCCA ARSA TAL 3002 & TAL 3003... asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA... TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA atg5 TAL3218 & TAL3219 TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA...

...Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Yellow...Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, ...pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f Syn...soma-jGCaMP8f none Constitutive 1, 9, rg* Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none ...AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Looger 176756 AAV-EF1a-jGCaMP8f-WPRE EF1a...AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8s 162374 pGP-AAV-syn-jGCaMP8s-WPRE...-jGCaMP8s-WPRE Syn jGCaMP8s none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG...

...victoria GGGCACGGGCAGCTTGCCGG 46760 cut S. pyogenes 23918387 Chen EGFP A. victoria GGGCACGGGCAGCTTGCCGG 47511...sapiens GCCCAGCCTCCGCACCTACA 61833 cut S. pyogenes 24424410 Ye gsk3b D. rerio GGGACCTGACCGGCCGCAGG 42245...sapiens GATACTGGAGAAGCAGGGGC 68466 cut S. pyogenes 26145478 Zhang PAX6 H. sapiens GTAGATTTTGTATGCACTGC 68465... PcfiA AAACAAAACCTCATCAGGCA 68900 interfere S. pyogenes 26918244 Lu PcfiA GAAGCTCACTCCTTAGCACG 68899 interfere...thaliana CACTAGGAGCAACACCCAA cut S. pyogenes 26479191 Kim P450 O. sativa CATATAGTTGGGTCATGGCA cut S. pyogenes...musculus ACAGGCATATGGTTGTCCAT 79005 cut S. pyogenes 26944679 Shaw Tfeb M. musculus AGCACTGTTGCCGGCCGAGG 79006...Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG 66989 cut S. pyogenes 26028531 Huangfu OCT4...

...Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m GCaMP7f GCaMP7s GCaMP7b GCaMP6f GCaMP6s GECO Other Biosensors...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium...-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn GCaMP8m Calcium...AAV-hSyn-Soma-jGCaMP8m Syn Soma-GCaMP8m Calcium sensor Fyhn 169258 AAV-hSyn-Soma-jGCaMP8f Syn Soma-GCaMP8f Calcium...-mDlx-jGCaMP8s-WPRE Dlx GCaMP8s Calcium sensor Looger 176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium...AAV-EF1a-jGCaMP8m-WPRE EF1a GCaMP8m Calcium sensor Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium...AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f Calcium sensor Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m Calcium...

...gfaABC1D-lck-GCaMP6f Biosensor Baljit Khakh AV-5-PV3435 100834-AAV5 pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 Biosensor...AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2817...

...158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...element Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...111261-AAV9 pAAV-hSynapsin1-axon-GCaMP6m For enriched expression of GCaMP6m in axons Lin Tian 111262-AAV1...AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched expression of GCaMP6s in axons Lin Tian 111568-AAV5 pZac2.1...

...OPEN gAGCATCACCtcgctGGAGGTGAGc PTRF/Cavin1 OZ513 and OZ514 OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1...OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l...OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (...CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc zgc:162148...CODA cTGCACCTTCACAAAAGGCCGCTGGCt LOC566040 OZ587 and OZ588 OPEN OPEN cAGCACCAGCCAGCCCAGAGTTGGGGt yap1 ...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...aGCCATCATCaggttcaGGATGAGCCc Kif6 OZ537 and OZ538 OPEN OPEN aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor...

...Cre-dependent GCaMP Kim , GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488... pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor GCaMP Kim , GENIE 135632 pAAV-S5E2-GCaMP6f E2 regulatory...104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE... Kim , GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn Calcium sensor GCaMP GENIE Optogenetics 26973 pAAV-hSyn-hChR2...regulatory element Calcium sensor GCaMP Dimidschstein 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor...