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We narrowed to 38 results for: AdRed

Showing: 1 - 20 of 38 results

  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...conceptualize where the populations are, try drawing a quadrant over the plot that divides it into four, using...different populations by imagining (or drawing) a quadrant over the dot plot. Created with biorender.com....Biorender! While a helpful visualization tool, a quadrant will often be too clean for real-world data. Most...these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this real-world...real-world data is far messier than biorender plots, quadrants can still help me as a reader if I'm struggling...strategy from Barlow-Anacker et al., 2017, with quadrants to help visualize the different populations. Used... This flow data, from Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend...pups born, with typical efficiencies of 30-60%. Quadros et al. attribute much of this variation to differences... locus may also influence efficiency. However, Quadros et al. saw success with Easi-CRISPR across three...Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with editing this blog post. References...PMID: 26242611 PubMed Central  PMCID: PMC4525291 Quadros, Rolen M., et al. “Easi-CRISPR: a robust method...Central PMCID: PMC5434640 Miura, Hiromi, Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka...

  3. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...and spread over the second quadrant. The process continues until all quadrants are used, hopefully revealing...plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick is dragged through...revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually successful...

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Dronpa that doesn't become inactivated as quickly. Padron is a monomeric, reversible PA-FP derived from mutating... / P141L / G155S / V157G / M159Y / F190S) (7). Padron’s “positive” switching behavior is the opposite ...opposite of Dronpa; when exposed to blue light, Padron is photoconverted from a dark state, to an activated state...bright green fluorescence. When exposed to UV light Padron is inactivated. The rs-Cherries (8) are another...UV-Violet Dark/Green N/A (high) High 503/ 518 Padron Monomer Blue Dark/ Green N/A (high) Low 503/ 518...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...experimentally-specific gates. Two-dimensional staining (quadrant gating) For our last example plot, I will show...cells (CD8+) from dendritic cells (CD11b+) using a quadrant gate. Within my “lymphocyte + single cell + live... two stains in relation to each other. Using a quadrant gate, I can divide my cell population into DN,...for each stain respectively). (B) Instead of a quadrant gate, I am free to use multiple individual gates...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...itself. Many thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University...

  7. What the HEK?

    Type
    Blog Post
    ...determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney...

  9. Genetic Code Expansion

    Type
    Collection
    ...Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed Badran 209740 pAB228q12...jannaschii Bacterial Ahmed Badran 209741 pAB228r3 Mlum1RS Bacterial Ahmed Badran 209742 pAB228p PylRS M. ...mazei Bacterial Ahmed Badran 209743 pAB228p4 PylRS M. mazei Bacterial Ahmed Badran 209744 pAB228v TrpRS...cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial Ahmed Badran 209746 pAMC070n1a12...MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran 212125 pBK-Ma pylRS nitroY/haloY-F5 PylRS M. alvus...

  10. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...the size limitations of ssODNs is Easi-CRISPR (Quadros et al., 2017). Easi-CRISPR in vitro transcribes...long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (2017). PubMed.  ...

  11. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    .... M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable...., Waterbury, A. L., Koblan, L. W., Kim, Y. B., Badran, A. H., & Liu, D. R. (2017). Improved base excision...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...receptor ADRB1R, B1AR, BETA1AR, RHR ADRB2 adrenergic, beta-2-, receptor, surface ADRB2R, ADRBR, B2AR, BAR...PAQR2 ADM adrenomedullin AM ADM2 adrenomedullin 2 AM2, FLJ21135, dJ579N16.4 ADRB1 adrenergic, beta-1-,...found on the surface of APC. Symbol Name Synonyms ADRM1 adhesion regulating molecule 1 ARM1, GP110, MGC29536...MSH-R, SHEP2 MC2R melanocortin 2 receptor (adrenocorticotropic hormone) ACTHR, MGC125798 MC3R melanocortin...

  13. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for cell surface...Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control for cell surface...

  16. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv 2020.03.29.013342 (Link opens...detection of SARS-CoV-2, including with crude enzymes. Bhadra S, et al. bioRxiv 2020.04.13.039941 (Link opens...

  17. Rett Syndrome

    Type
    Collection
    ...Research Milestones 1992 - MECP2 is discovered by Adrian Bird's lab. (Link opens in a new window) PMID: ...Link opens in a new window) PMID: 10508514 2007 - Adrian Bird's lab demonstrates that Rett syndrome symptoms...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 206555 Anti-Alpha-2C adrenergic receptor [N330A/80R] Alpha-2C adrenergic receptor Human Mouse IgG2a... Mouse IgG2a 220412 Beta-2 adrenergic receptor [N430/30R] Beta-2 adrenergic receptor Mouse Mouse IgG2a...scFv] N324/2 Rem2 Rat Mouse 219492 Anti-Alpha-2C adrenergic receptor scFv [N330A/80 scFv] N330A/80 Alpha-...
Showing: 1 - 20 of 38 results