...submit deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process...Online Deposit” and “Download Deposit Spreadsheet” (see Figure 1). Please read our “How to Deposit Your ...Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet.” If...Start Your Deposit Deposit FAQ Which Plasmids Should I Deposit? ... If you’re depositing 10 or more plasmids, the Deposit Spreadsheet is the quickest option. Simply download... need to deposit a collection that is greater than 75 plasmids, please contact us at deposit@addgene.org....org. These deposits are considered large deposits and are exceptions to our standard workflow.  Table...

... researchers who deposit with us!  This year alone, we had 12,427 plasmids deposited with us. We cannot...Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday...to all our depositors: thank you so much!!!  (A special shout-out to those who are depositing their data...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday: Thank you post (this is...Friday: Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there...

...detectable by two different tags: a reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti...independent of the reporter. The only caveat is that HA staining is weaker than reporter staining. Picking...Picking the right reporter for your RANBody While all three reporters stain cultured cells and tissue sections...Monster, and Chicken IgY Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens...genetically tagged and instead are conjugated to reporters (i.e. fluorescent dyes, HRP, etc. ) after purification... is a RANbody? A RANbody is a fusion between a reporter and a nanobody that’s encoded on a plasmid. A ...sections, some reporters are better suited for particular applications. Read on to learn more about each...

...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some...written by Addgenies and some are written by the depositors themselves. All of them have cool new tools, ...scientists to use. As always, a big thank you to our depositors for sharing your science with us! Corralling ...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...one of our favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've...

...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more interestingly...plasmids that the authors deposited with Addgene and those without plasmids deposited with Addgene. Together...plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited with...Addgene deposits as if they were published in the same journals as those that did make deposits to Addgene...addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. Since then...interestingly, could we tell if depositing a plasmid with Addgene causes a paper to get cited more?   ...scientific articles associated with Addgene plasmid deposits are more highly cited, we went to one of the largest...

...notes) Any Data repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base...Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository See...there are websites that can help: Antibody data repositories, which share validation data, and antibody search...using antibody search engines and antibody data repositories to find an antibody for your experiment. Search...available antibodies from many vendors, while data repositories share validation and experimental data to help...familiar with in Table 1.  Table 1: Antibody data repositories and search engines Website Targets Application... Notes Antibodypedia Any Any Data repository   YCharOS Human proteins Immunoblot (...

... CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying...histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct...Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems provide ...Recently scientists have created CRISPR fluorescent reporter assays to quantify targeted Cas9 cleavage, and...and non-homologous end joining (NHEJ). In these reporters, editing typically results in a change in the ...this blog we will focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover... the Piggybac transposon to stably integrate a reporter gene that contains a puromycin resistance gene...

...the deposit process by clicking "Submit Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid...know by contacting deposit@addgene.org. These deposits are considered large deposits and are exceptions... you've deposited with us and distribute them to scientists worldwide. What's more, depositing is free...as a step-by-step guide to the online deposit process. To deposit plasmids online, you will need to create...account, you can begin the deposit process by clicking "Submit Online Deposit" (for online submission) ...) or "Download Deposit Spreadsheet" (for emailed submission) in the Deposit menu: Figure 1: Begin the... plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid...

...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...through a repository, they remain accessible during these times. Centralized reagent repositories, also known...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the rapid... the scientific community can support repositories. Repositories are essential for the advancement of ...original sources. Figure 1: Biological repositories that distribute mouse strains, plasmids, cell...Culture Collection (ATCC) is a private, nonprofit repository for a variety of materials for life science research... sharing and distribution of cell lines from repositories on opposite sides of the world shows that science...

...Tuesday: Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday...plasmid tools! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make...bulk of these plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that... as an example). Depositor Week will be fun for everyone, whether you are a depositor or not. We will ...started from 474 plasmid deposits in 2014 which has grown to ~10,000 plasmid deposits per year in recent years...for some Addgene depositor fun facts for a quick way to grasp the big impacts depositors have on science...your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday. There...

...fluorescent executioner caspase reporters. Several apoptosis reporters have been developed, such as the...transfer (FRET)-based executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase...FlipCherry: Red fluorescence protease reporter To add to the fluorogenic reporter toolkit, the Shu Lab also designed...novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves approximately...fluorescence emission. FRET and fluorescence based reporters however have several limitations. First, FRET ...study apoptosis in vivo scientists need a protease reporter that has a large dynamic range and high brightness...Shu Lab from UCSF designed an improved apoptosis reporter, ZipGFP, which utilized the self-assembling split...

...appreciate our depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who ...way we can gauge if people are happy depositors is if they deposit plasmids more than once. People get ...tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a deposit. Begin yours here....Start a Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each...world choose to share their plasmids through our repository since we know biological resource centers, including...

...real time, but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome these...existing bacterial reporters Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint what...signal is coming from.   Acoustic reporter genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene...imaging tools The luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org... researchers have developed in vivo bacterial reporters that use fluorophores and luciferases to track...these limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available...

...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used... to study gene expression in cells. But these reporters have limited use in vivo because they are based...expression in vivo.  But what ultrasound lacked was reporter genes that could produce ultrasound contrast. ...our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs), which are sets of genes that, when...5202–5209 (2017). Bourdeau, R. W. et al. Acoustic reporter genes for noninvasive imaging of microorganisms... Hurt, R. C. et al. Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors...

...sending the phages to the clinicians. Creating a repository of phage data and information Since the inception... their role in the phage community by being a repository of phage data and information. “We want to be...

... intact. Some deposits are sent from labs nearby in Boston and Cambridge. Many deposits arrive from labs...an average of 10 plasmids per deposit. Each week an average of 16 deposits is received in the lab, although...help move your deposited samples along every step in the storage process - from deposited DNA to transformed...transported across oceans and continents. Each deposit may consist of anywhere from one to hundreds of... prepares the sample for entry into Addgene’s repository. For long-term storage in our facilities, every...different types of samples at Addgene - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready...transformations. The lab typically transforms newly deposited samples from Monday through Wednesday. Each sample...

...some good news with you: with contributions from depositors around the globe, we’re now sharing over 100,...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, ...currently at 1,484,507 as of April 8, 2021) 5,000 depositing labs (currently at 4,651 as of April 8, 2021)...

... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance... editing. eGFP reporters for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9...DSB in their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target...editing reporters. Image from St. Martin et al., 2019. The Harris lab used these eGFP reporters to analyze...Transient reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient reporter for...cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid delivery... functional reporter system to identify and enrich for base-editing activity The reporters mentioned above...

...you out. What is the Open Repository of CRISPR Screens? The Open Repository of CRISPR Screens (ORCS) database...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...research. Fortunately, there’s a growing open repository of CRISPR screen data that might be able to help... resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in order to...Original published CRISPR screen data is curated and deposited in BioGRID ORCS to allow facile queries of published...biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS) homepage. Screenshot...Screenshot updated 2024.   Using the Open Repository of CRISPR Screens BioGRID ORCS currently houses over ...

...Problems with FRET kinase reporters Difficult to Multiplex: FRET reporters are comprised of two fluorescent...phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded...single-cell kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex...alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a...multiplexed together. Challenging  to Design: FRET reporter activity depends on both the distance and the .... Slower Dephosphorylation Rates: FRET-based reporters can have slower dephosphorylation rates than KTRs...KTRs which is hypothesized to be caused by FRET reporters having phosphorylated conformations that are less...