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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 43 results for: NAK

Showing: 1 - 20 of 43 results

  1. Plasmids for Stem Cell Research

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    ...research led to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent...8081. Kay and Wu MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral...defined factors. Cell. 2007 Nov 30. 131(5):861-72. Yamanaka Plasmid Human Non-integrating transient expression...Peripheral Blood Cells. Stem Cells. 2012 Nov 29. Yamanaka Replicating EBNA1 episome Human Non-integrating... iPS cells. Nat Methods. 2011 May;8(5):409-12. Yamanaka Replicating EBNA1 episome Human Non-integrating... MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral...defined factors. Cell. 2006 Aug 25. 126(4):663-76. Yamanaka piggyBac Mouse Doxycycline-inducible piggyBac ...

  2. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote... chance. Here was an opportunity to see Shinya Yamanaka celebrate the tenth anniversary of his landmark... and cMyc (the so called OSKM factors). As the Yamanaka lab's primary Addgene contact for many years, ...community – a community largely created due to Yamanaka’s seminal work. It didn't take very long to realize...realize that the effect of Dr. Yamanaka's work has been profound, and this is a rare instance in which...thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer...stepped back to give a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with ...

  3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    Blog Post
    ...generated from mouse fibroblasts in 2006 in Shinya Yamanaka’s lab, where the reprogramming factors were delivered... was taken forward the next year when both the Yamanaka and Thomson labs showed the successful generation...These stellar works led to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs...PubMed PMID: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse...

  4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...General Hospital. A little over a decade back when Yamanaka and colleagues reported that it is possible to... (iPSCs) by the addition of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative...19668191. PubMed Central PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming... Enhanced Reprogramming Find plasmids from the Yamanaka Lab ...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post
    ...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

  6. Tips for arabidopsis transformation

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    Blog Post
    ...couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used...(basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively, if you prefer Golden...25902487. PubMed Central PMCID: PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors...

  7. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post
    ...short dsRNA. However, it is important to note that naked and unmodified dsRNA elicits immunogenic reactions...Physcomitrella) patens (Bezanilla et al., 2005; Nakaoka et al., 2012).     A new positive selection-based... 34:634–636 . https://doi.org/10.1038/nbt.3567 Nakaoka Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse...

  8. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization? Codon...  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura Y (2000) Codon usage tabulated from international...

  9. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ..., Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV,...Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F,...

  10. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels of the second messenger...23488798. PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin...

  13. Delivery Methods for Generating iPSCs

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    ...Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression...Find reprogramming plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific...

  14. Using AAV for Neuronal Tracing

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    Blog Post
    ..., H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local and retrograde gene transfer...Central PMCID: PMC5102064. Roberts, T. F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah...

  15. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...dye front (and many ladders!) are visible to the naked eye.   If you see a smiley face in your gel, or ...you can use a colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging...

  17. KLF Research Plasmids

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    ...Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs...

  19. CRISPR Guide

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    ...Gootenberg, J. S., Mori, H., Oura, S., Holmes, B., Tanaka, M., Seki, M., Hirano, H., Aburatani, H., Ishitani...., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda,...
Showing: 1 - 20 of 43 results