We narrowed to 216 results for: Rras

...Zinc Finger Arrays Zinc Finger Consortium: Zinc Finger Arrays Engineered Zinc Finger Arrays for Endogenous...zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs targeted to a...Peterson, and Joanna Yeh are engineering zinc finger arrays targeted to various zebrafish genes. The table...ZFN expression vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through...more details). IMPORTANT NOTE: All zinc finger arrays listed show transcriptional activation activity...ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target Site (5' to 3') ...

...avoid being caught in the antibody crisis, one must corral and corner the goldilocks antibodies. A goldilocks...

... gene editing. They named this new array architecture the DAP array, short for “drive-and-process.” To...the activity to release gRNAs from DAP array.7 Using DAP arrays with a variety of base editors, the Gao...Schematic of multiplex gene editing with DAP array   Use DAP arrays can enable efficient multiplex gene editing...from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream...upstream lengthy promoter to express the gRNA array, DAP arrays use tRNA itself, as short as 75-nt, to both...sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications...encodes multiple guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen...

...protocol is provided for your convenience. See “warranty information” in appendix. Table of Contents A....Sequence of pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning ...library for human and mouse genes applied to an arrayed viral high-content screen . Moffat J et. al. Cell... 0.22 μm filter. Store aliquots at -20°C. I.3. Warranty Information Addgene is committed to providing ...typographical or other errors may occur. Addgene makes no warranty of any kind regarding the contents of any literature...and on an “AS IS” “AS AVAILABLE” basis without warranty of any kind either expressed or implied, including...including but not limited to the implied warranties of fitness for a particular purpose, non-infringement...

...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific treatments...

...expressing Cas9 and a gRNA will result in a diverse array of mutations (see: Validate genetic modification... a given genome. Some more recent libraries use arrays of multiple gRNAs to target paralogs or lncRNAs...R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome ...Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G., & Zhang, F. (2013). DNA targeting...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature , 516 (... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays have been... the final array is composed of 18 TAL effector repeats. The kit can be used to make arrays from 12-31...nucleotide triplets. With some trial and error, ZF arrays are able to recognize their targets with fairly... fused to a nuclease or activation domain, a ZF array is an effective targeting mechanism for molecular...and interactions between zinc fingers within an array can reduce their specificity. Two years ago, groups...importance of context for each TAL effector within an array, but early studies suggest the likelihood of context...negative effect on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation...

...Parkinson's Elisa Izaurralde 147087 pLNHA-C1-HseIF4G_O EIF4G HA CMV Parkinson's Elisa Izaurralde 147091 pT7-...Parkinson's Elisa Izaurralde 148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113 pcDNA3.1...proteinopathy, ALS Elisa Izaurralde 148138 pT7-V5-SBP-C1-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148139 pT7-V5...ALS Elisa Izaurralde 148154 pnEA-NvM-DmeIF4G_578-650-GB1_Z EIF4G Parkinson's Elisa Izaurralde 148183 pnEA-NvG-DmeIF4G...'s Elisa Izaurralde 148504 pnEA-NpM-DmeIF4G_578-675-GB1_AD EIF4G Parkinson's Elisa Izaurralde 148505 pnEA-NpM-DmeIF4G...Parkinson's Elisa Izaurralde 148506 pnEA-NpM-DmeIF4G_601-660-GB1_AD EIF4G1 Parkinson's Elisa Izaurralde 148507 pnEA-NpM-DmeIF4G...'s Elisa Izaurralde 148508 pnEA-NpM-DmeIF4G_617-634-GB1_AD EIF4G Parkinson's Elisa Izaurralde 148509 pnEA-NpM-DmeIF4G...

...Extension, Volume I Bacterial Expression Richard Murray 110 plasmids designed to quickly and efficiently...efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats....Genome Engineering, TALEN Takashi Yamamoto Nine pFUS array vectors and destination vectors designed for use...

...into contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process...CRISPR system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that ...short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA...cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P,..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering...Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath ...gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol . 35(1):31-34. PMID: 27918548...

...PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...

...system using two cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013... SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression... indicated below) can be cloned into the CRISPR array. The oligos are designed based on the target site..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126...V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;... 10.1016/j.cell.2013.08.021. Epub 2013 Aug 29. Erratum in: Cell. 2013 Oct 10;155(2):479-80. PubMed . Genome...

...Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI...Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter vector...cloning DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH ...

...expressing Cas9 and a gRNA will result in a diverse array of mutations (see: Validate genetic modification... a given genome. Some more recent libraries use arrays of multiple gRNAs to target paralogs or lncRNAs...R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome ...Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G., & Zhang, F. (2013). DNA targeting...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature , 516 (... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular... Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc...

...Institute Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets...DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is ... can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with...

...three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme...of these platforms assemble TAL effector repeat arrays on a single architectural framework and the DNAs... are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs which...

...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular... 2017 Nov 10;8(1):1412. Justin Taraska , David Perrais pH Red pH-sensitive fluorescent protein pHmScarlet...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...

...pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF, 129 KB Marraffini Bacteria: pCRISPR...pSL1180polyUBdsRED PDF, 597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9...

... nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors.... nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular...