We narrowed to 419 results for: SAM-2

...references are below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library...please visit the Addgene SAM library page . Targeted gene activation using SAM There are two sets of 3 ...CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( ...information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids and screening...library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional...available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed for...sites for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo...

... has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc Firefly EF1α ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion...

...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006...the genomic region you intend to target in your samples to ensure there are no discrepancies with the reference...

...none 2, 5 Christopher Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, 5, rg...(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 44362 pAAV-hSyn-DIO-hM4D...(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan Roth 50475 pAAV-hSyn-hM4D(Gi)-...mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan Roth 154867 pAAV-hSyn-fDIO-hM4D...Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Bryan Roth 50476 pAAV-CaMKIIa-hM3D(Gq)-mCherry ...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9 Bryan Roth 50477 pAAV-CaMKIIa-hM4D(Gi)-mCherry...

...of basal H 2 O 2 levels with peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with...Belousov Hydrogen Peroxide (H 2 O 2 ) Cytosolic or mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1) ...intracellular Zn 2+ homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn 2+ FRET sensor...peroxiredoxin-2-based probe. Nat Commun. 2018 Aug 7;9(1):3145. Hadley Sikes Hydrogen Peroxide (H 2 O 2 ) Monitoring... for Ex Vivo and In Vivo Imaging of Ca 2+ Dynamics in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun ...Cameleon calcium sensors Bcl-2-mediated alterations in endoplasmic reticulum Ca 2+ analyzed with an improved...encoded Ca 2+ indicator with enhanced two-photon absorption. Neurophotonics. 2024 Apr;11(2):024207. Robert...

...immunoglobulin heavy diversity 2-15 D2, IGHD215 IGHD2-2 immunoglobulin heavy diversity 2-2 IGHD22 IGHD2-21 immunoglobulin...heat shock 70kDa protein 2 HSP70-2, HSP70-3 HSPA4 heat shock 70kDa protein 4 APG-2, HS24/P52, MGC131852, ...variable 2-33 (non-functional) IGLV233, V1-9 IGLV2-8 immunoglobulin lambda variable 2-8 IGLV28, V1-2 IGLV3...beta 4 DEFB-2, DEFB102, DEFB2, HBD-2, SAP1 EDN1 endothelin 1 ET1, HDLCQ7 EDN2 endothelin 2 ET2, PPET2 ...receptor subfamily 2, group C, member 1 TR2 NR2C2 nuclear receptor subfamily 2, group C, member 2 TAK1, TR2R1...suppression of tumorigenicity 2 - STC1 stanniocalcin 1 STC STC2 stanniocalcin 2 STC-2, STCRP TAC1 tachykinin,...FLJ99988, FLT2, HBGFR, KAL2, N-SAM, OGD FGFR2 fibroblast growth factor receptor 2 BEK, BFR-1, CD332, CEK3, ...

...Library 111708 Mode #2 Library (NEDD4-2 WW2 domain) Pooled Library 111707 Mode #2 Library (NEDD4 WW2 domain...phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library configuration for overexpression...Pooled Library 111706 Mode #2 Library (14-3-3σ) Pooled Library 111705 Mode #2 Library (14-3-3β) Pooled Library...phosphorylation-dependent protein-peptide interactions (“Mode #2” expression, Barber et al., 2018). The phosphosite...fluorescence-activated cell sorting (FACS). The Mode #2 phosphosite library contains four different phosphobinding... WW2 domain of NEDD4, and the WW2 domain of NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The...iSPI_pSer_Subpool#3 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188526 iSPI_pSer_Subpool#1 Plasmid ...

...suggests that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard...catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre... a new vial of early passage cells. Approximately 2 weeks post-thaw, cell culture supernatant is tested...of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample. Sterility Viral vector preparations are added ...rigorous barcode matching system that follows the sample from the DNA tube all the way to the cleared viral...

...interface upon thaw (Fig. 2). Before thawing, all the samples were at the control pH of 7.4 as indicated by ...showed that AAV infectivity was not altered by a short 2-hour exposure to pH as low as 2.5 (Potter et al., ...Methods. 2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet...affected the pH of our viral samples. Read on to learn how aqueous samples might be affected by dry ice...Measuring the pH of our samples: What we did To measure the pH of our samples, we used a solution of cell...when the samples were frozen. This was clear by the gradual change in pH visible at the sample interface...at -80°C. For samples that are shipped on dry ice, this can affect the pH of the sample, which may have...

... mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett syndrome is an X-linked disorder...X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet . 23, 185–188. (Link opens in a new window... Cuddapah et al. 2014. Methyl-CpG-binding protein 2 (MECP2) mutation type is associated with disease severity... Specific mutations in methyl-CpG-binding protein 2 confer different severity in Rett syndrome. Neurology...males with mutations in Methyl-CpG binding protein 2. Am J Med Genet B Neuropsychiatr Genet . 180, 55–67...variability in symptoms even within patients with the same mutation, the location and type of MECP2 mutation...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...

...overview of the most common methods. BioBrick BioBrick-2 BglBrick Silver Standard Freiburg Standard BioBrick...visit iGEM: BioBrick (RFC 10) Back to Top BioBrick BB-2 (RFC 12) Prefix: GAATTC GCGGCCGC T ACTAGT G Prefix...XbaI, SapI For more info, visit iGEM: BioBrick BB-2 (RFC 12) Back to Top BglBrick – Berkeley Standard ...assembly, even as more parts are added. Therefore, the same reaction which ligates a promoter into an empty ...

...domains used in SAM. This method has been shown to activate genes at 2- to 3-fold higher than SAM, SunTag, and...activation. Synergistic Activation Mediator (SAM) Description SAM uses specially engineered sgRNAs to increase...re-engineer components of Cas systems themselves. SunTag, SAM, and VPR have all shown significant improvements ... p65). Performance When targeting single genes, SAM consistently shows the highest levels of gene activation...regulation (activating multiple genes at once), however, SAM exhibits activation levels comparable to other popular...activators while showing lower activation levels than SAM. One drawback of this method is its construction:...VP64 activator, but lower levels compared to the SAM system. Its activation levels are similar to that...

...CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional...VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2...not been achieved.” That is, until now. Enter the SAM pooled library In a paper published in Nature in ...plasmid which expresses the activation helper protein. SAM can be combined with a human genome-wide library ...Nature paper reports, “Here we have shown that the SAM system is robust, specific, and can facilitate genome-scale...combined with a compact pooled sgRNA library. Our SAM-mediated screens exhibited a high degree of consistency...References & Further Reading: For more information about SAM, including a tool for getting optimized guide sequences...

...sites on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...protein, instead of a two-component system (Figure 9C) SAM activators — co-expression of dCas9-VP64 with a modified...Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview of the NHEJ repair mechanism. Multiplex ...Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...

...cells; function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible ...such as anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found...secondary immune response Monomer with a valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary... ranging from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include...used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link the samples with formaldehyde...

...transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs...2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I (Cas3) Type ... with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA...regulation of transcription in eukaryotes. Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino...Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell . 163(3):759-71. PMID: 26422227...targeting multiple loci can be easily expressed in the same cell or organism. The ability to precisely target...

...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...+J phagemid libraries. Immunol Lett. 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for...microscope, or detect when the protein is present in a sample — just to name a few. Traditional sources of antibodies...

... Testing Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8...Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, ...

...extraction protocol for more details.   Figure 2: An uncut plasmid is expected to form 3 bands: nicked...replicate.   How can I prevent recombination in my samples? Plasmid recombination can affect viral vectors...stable E. coli reduce the rate of recombination in samples. For a more detailed comparison of these cloning... easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that...

...that numbers are not switched around or misread.  2. Track the container - It is just as important to ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the.... Some samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received...every sample can be tracked easily at each step in the storage process. After the original sample is barcoded...scanned to verify that it is the correct sample. Tracking samples - it's all about location, location, location...these samples have the 2D barcodes mentioned above. These barcodes identify what type of sample is contained... on the sample type. It is critical for us to know which plate, rack, and freezer each sample is located...