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We narrowed to 53 results for: barcode

Showing: 1 - 20 of 53 results

  1. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...and make sure they have been barcoded with a unique ID number (1D barcode as shown below). This number...all of these samples have the 2D barcodes mentioned above. These barcodes identify what type of sample is... can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube Plate Freezer Rack Freezer Tubes...A1 through H12. Each plate is also barcoded with a 10 digit barcode and then assigned to a location in...every barcode on every tube contains information about that tube’s appropriate location. The barcode also...plated onto a petri dish plate that is also barcoded. The barcodes on both of these are scanned into our LIMS...within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible and can be made...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...individual cell receives only one barcode. The abundance of each barcoded clone can then be monitored over...over time by sequencing the barcodes in the population (all barcodes can be amplified using the same sets...Looking at barcode abundances among multiple replicate populations, they found that similar barcodes consistently...with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells' genomes...ClonTracer system, lentiviruses containing these barcodes infect the target cell population at low multiplicity...reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance of a...specific clone; lineage tracing is performed via barcode sequencing. Using the ClonTracer library, Bhang...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    ...visual barcodes for clonal multiplexing of cells in live imaging application. Each visual barcode consists...visual barcodes in use in A375 cell line. Image from Kaufman, et al, 2022.    This new barcoding technology...experiments with one visual barcode per cell and up to 200-plex with two barcodes per cell. The researchers... error rate for long sequence knock-ins Visual barcodes for live cell clonal multiplexing Improving ...https://doi.org/10.1038/s41556-021-00836-1 Visual barcodes for live cell clonal multiplexing by: Rachel Leeson...fluorescent proteins, and inserting each visual barcode into its own subclone, they were able to develop...kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman, et al. 2022. Nature Comm.https...

  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...Get on that barcode gravy train Buy a barcode label printer. Buy barcode scanners. Barcode everything....another. If you are using barcodes (and you should be!) bear in mind that your barcodes are being applied to...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff...staff. Barcoded materials make everyone's life easier, the time and capital cost of getting up to speed...containers, not to the contents. Tightly coupling barcodes and containers might not be such a bad idea, tightly...Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab Get Tips on How to Keep a Lab Notebook...

  5. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...and its 8-bp barcode, respectively. Once cloned into the storage vector, the pooled, barcoded gRNA library...The CombiGEM platform provides a means to create barcoded gRNA libraries that can be used to combinatorially...restriction digestion and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs...quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can accommodate...! Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from Wong et al., ... sequences, you can then readily generate your barcoded gRNA library sequences via oligo synthesis using...experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted from Wong...

  6. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...equal distribution. Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents...range from technical tips as in our episode on Barcoding, to basic lab etiquette that will keep the lab...reagents in your lab. With barcode tracking, you will always be able to locate materials quickly and easily...easily. Learn more about using barcodes. Check out our “Lab Tip” filter on the blog to keep up to date...

  7. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...fragments that are then barcoded for identification. This mixture of barcoded fragments is known as a ...large number of samples are subjected to a DNA-barcoding step that uniquely labels DNA from each well of... containing fragments carrying sample-specific barcodes. Rather than continuing to process 100s to 1000s...

  9. Lentiviral Prep Service

    Type
    Collection
    ...) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version... the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially...of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially...of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially...Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made...direct fluorescence. Morris 115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral particles...direct fluorescence. Morris 115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral particles...

  10. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...tagging method that labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6...Guo, Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics...

  11. Pooled Library Amplifications

    Type
    Blog Post
    ...Culture Re-Amplification Protocol (PDF, 71 KB) Barcode Watermelon Library 5,000,000 30 bp At least... Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 ...Library 3,548 250 - 10,000 bp, plus a 24 bp barcode 250–1,000 ng Solid Joung et al. Nat Protoc...Pooled Library 80,962 1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification...

  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...genome editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids...025-01989-9.   Make a splash with new LARRY barcoding libraries By Mike Lacy Whether you’re planning... libraries for any summer project. While many barcoding libraries are available for tracking cell lineage...their updated Lineage and RNA Recovery (LARRYv2) barcoding libraries. The team used these libraries to analyze...

  13. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...library of each unique TF open reading frame (ORF), barcoded for tractability. These ORFs are individually ...generated (Joung et al., 2023). Fig. 1: Barcoded TF library was packaged in lentiviral particles... and screening mechanisms, although the unique barcodes for each TF help streamline identification. While...

  14. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...and moved to our packing area.Using the barcode system, the correct plasmids are packed into their boxes...

  15. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for...transcription factor isoform has a unique 24 bp barcode that simplifies identification of the transcription... open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification...

  17. Viral Production at Addgene

    Type
    Blog Post
    ...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...

  18. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...CombiGEM is a technology that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong... Lab at MIT) shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... carefully track samples through our lab using barcoded tubes and plates. The critical pieces of information... to Verify Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website...

  20. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...engineer that you want the LIMS software to create a barcode for your plate, you will get in trouble if you ...given container Whether or not the container was barcoded (this is pretty Addgene specific) The types or...
Showing: 1 - 20 of 53 results