Skip to main content

We narrowed to 416 results for: iCre

Showing: 1 - 20 of 416 results
  1. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Layer 2-3_IT Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT ...Layer 2-3_IT Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex...Layer 2-3_IT Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT ...Layer 4_IT Isocortex 224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_...Layer 4_IT Isocortex 220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_...Layer 5_IT Isocortex 220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_...Layer 5_ET Isocortex 229920 pAAV-AiE0459m_3xC2-minBG-iCre(R297T)-BGHpA AiP14187 AiE0459m_3xC2 Cre Layer 5_...
  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express... pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato... when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed with...Express iCre under from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and ...pAdx-CMV-iCre-P2A-tdTomato 73351 Expresses iCre and tdTomato from the CMV promoter pAdxEF1-FLPe-tdTomato 73352 Expresses FLPe...
  3. Caltech Systemic Capsids

    Type
    Collection
    ...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA minBG iCre expression iCre Ting 199775 AiP13778 - pAAV-AiE0450h-minBG-iCre...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) minBG iCre(R297T) expression iCre AIBS , Ting 199776...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) minBG iCre(R297T) expression iCre AIBS , Ting 199777...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) minBG iCre(R297T) expression iCre AIBS , Ting 214862...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) minBG iCre(R297T) expression iCre AIBS , Ting PHP.S... Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV ...
  4. Microbiology Resources

    Type
    Collection
    ...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene’s ...not listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...repository contains plasmid tools for a range of microbial fields, including bacteria, viruses, parasites...species you work with, or check out our additional microbiology resources. The plasmids Addgene distributes ...cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard...
  5. Brain Armamentarium

    Type
    Collection
    ...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...Capsid) 230681-AAV5 AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA AiE2116m is an enhancer sequence, designed...Gradinaru 199777-PHPeB AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre...199776-PHPeB AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre...Gradinaru 199775-PHPeB AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre...Gradinaru 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in...
  6. CRISPR Antimicrobials

    Type
    Blog Post
    ...remain in the delivery of these agents, CRISPR antimicrobials could become our newest line of defense against...the growing problem. Above all, we need new antimicrobials, ideally ones for which the development of ...process essential to most bacteria, custom CRISPR antimicrobials can target specific sequences in a single virulent... Bikard et al. developed sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik...particles using a helper system. These CRISPR antimicrobials successfully resensitized a beta-lactam resistant...observed substantial resistance to the CRISPR antimicrobials, and they suggest that targeting multiple resistance...these techniques is efficient delivery, as the antimicrobial must reach essentially every cell or else small...
  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... techniques like super-resolution microscopy or electron microscopy. Since its introduction in 2015 by...publication.         Why Expansion Microscopy?  Traditional light microscopes are limited in the level of resolution...for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously...synapse by conventional light or fluorescence microscopy (Dani, et al., 2010). How do you overcome this...this resolution limit? Electron Microscopy (EM) is one option, but the equipment needed for EM is not something...targets you can label in a sample. Super-resolution microscopy (SRM), a family of imaging techniques developed...
  8. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...word out about a particular microorganism, microbial technique, or microbial technology, sign up to write...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be sure... resource page for the Microbiology community. On our new curated Microbiology Collection page, you can...resources for microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with...out how to use genome editing techniques in your microbe of choice. For more help with CRISPR, read our ...find plasmids for your work, plasmids in the Microbiology Collection are sorted by organism (see screenshot... microbiology...
  9. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...looking at’ to ‘listening to’ microbes But seeing microbes through a microscope is not the only way to visualize...nope, not at the American Society for Microbiology 2018 Microbe meeting. Soon after I arrived, I was looking...improv workshop. These paper microscopes work as conventional microscopes and give a similar optical quality...140X and 2 micron resolution). I have worked many hours in a dark room with a confocal microscope, yet I ...through a paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford...when looking down a paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful...Caltech presented an exciting way of listening to microbes by using non-invasive methods for cellular imaging...
  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... switch microbiota/biomarker sensors here Studying the bee microbiome using the Bee Microbiome Toolkit...what the microbes are doing. But aside from sequencing, scientists can also study the microbiome by using...reporter microbes. Let’s take a look at a few of these methods below. Engineering gut microbes in situ...monitor the microbiome with sequencing is vast, the ability to genetically alter microbiome members is ...number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins...developed the Bee Microbiome Toolkit (BTK) to study bumble bee and honey bee microbiome. The toolkit consists...the bee microbiome, these broad-host-range plasmid could also be used to study other microbiome systems...
  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Light sheet fluorescence microscopy vs. confocal microscopy In confocal microscopes, optical sectioning of... light microscopy, particularly related to the neurosciences.  These developments in microscopy coupled...One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). The underlying ...light sheet fluorescence microscopy Modern light sheet fluorescence microscopy was first pioneered by Voie...cleared rodent brain, the Ultramicroscope was developed using a stereomicroscope to capture large field-...www.lavisionbiotec.com/ultramicroscope-overview.html http://www.zeiss.com/microscopy/en_de/products/imaging-systems...techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture of whole...
  12. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ..., we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...attended the American Society for Microbiology's annual meeting (ASM Microbe 2016) for the first time. Our...on the horizon for microbiology research and we’d love to help more microbiologists share these valuable...resources! Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene... out Our Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...recently begun expanding our presence in the microbiology community. For our first concrete steps into...little bit of what we learned. Check Out Our Microbiology Page! The start of the conference After setting...
  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia. PNAS 97, 1813–1817...machines that can extend and retract over many microns in response to pH changes without the need for ...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...bodies could also perform as actuators in microelectromechanical systems (MEMS) devices. They could be functionalized...
  14. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent Widefield Microscopy is the...photon microscopes. Total Internal Reflection Microscopy (TIRF) Total Internal Reflection Microscopy (TIRF...fluorescent microscopy. Collimated (non-converging or diverging) excitation light exits the microscope objective...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy Point...Point Scanning Confocal Microscopy was the first fluorescence microscopy technique to incorporate optical...Parallelized Confocal Microscopy (Spinning Disk) Parallelized Confocal Microscopy (Spinning Disk) increases...confocal microscopes use parallelization to enhance performance. In spinning disk microscopy, a metal ...
  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...backstage team. Meet the microenvironment, a tumor’s backstage team. Microenvironments are cellular regions...cancer microenvironment Ever since Stephen Paget’s “seed and soil” theory in 1889, microenvironments have...Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009): 9-17...specialized structures. Tumors are surrounded by microenvironments and what encircles a disease is highly prone...precisely that for breast cancer. What are tumor microenvironments made of?  A tumor’s genesis is divided into...initiation, progression, and metastasis. The microenvironment’s physiology is deeply connected to these 3... soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming influence...
  16. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...your experimental results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination...codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate higher...
  17. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...Acoustic reporter genes for noninvasive imaging of microbes in mammalian hosts Article contributed by Lukas...are beginning to find connections between the microbiome and diseases, the immune system, and other vital...vital systems in the body. When a microbe travels through a host organism, the expression of some genes...methods for visualizing the location and function of microbes rely heavily on fluorescent protein genes. These...provide a major benefit to scientists studying microbiome systems that are potentially problematic for ...podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...
  18. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Larsen New serotype AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases...viral prep AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases... New viral prep AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases...
  19. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...continents and research diverse topics including microbiology, brain injury, drug discovery, and synthetic...Cayetano Heredia, Peru Patricia Sheen, Professor in Microbiology, and her group are interested in understanding...biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach, plasmids... proteins and a new two-photon laser scanning microscope will allow Tsakanova to image cortical neurons...Additionally, optogenetic plasmid tools and non-linear microscopy methods will be used to stimulate and manipulate...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ... employing synthetic biology resources and microfabrication technologies to generate a self renewable ...
  20. Recombinases AAV Preps

    Type
    Collection
    ...Carmichael 214848 AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) minBG N/A 2 Allen Institute...pAAV-EF1a-C-CreintG EF1a none 1 Connie Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP...Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV...
Showing: 1 - 20 of 416 results