We narrowed to 130 results for: primer

1. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   Blog Post

   ...or toe clip) and using PCR with the appropriate primers to detect your targeting vector or gene of interest...

2. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ... Addgene Blog New to Virus Work? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about...

3. Plasmids 101: FLEx Vectors

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   Blog Post

   ...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

4. Plan Your Experiment

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   Collection

   ... region of interest can be PCR amplified using primers that (A) flank the region of interest (deletions...

5. The Materials Science of Optogenetics Experiments

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   Blog Post

   ...stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into some of the important...

6. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...epitope tag or fusion protein. If your sequencing primer was chosen to confirm a tag or fusion protein is...

7. 28 Hot Plasmid Technologies from 2015

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   Blog Post

   ...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...effective. Their Snapdragon tool helps you select primers to synthesize RNA for gene silencing. Additionally...

9. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...Adenovirus Associated Virus New to Virus? Check out Our Primer for Beginners! Learn All about Lentiviral Vectors...

10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene: Why is it so important ...