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  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria...in the recipient cell, the CRISPRi DNA (purple) flanked by left and right Tn7 end sequences (green) integrates...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...cells. These plasmids use fluorescent proteins flanked by long regions of homology to the gene of interest...and protein-protein interactions. Additionally, thanks to the modular arrangement of vectors included ...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (... system under normal and diseased conditions.  Thank you to our guest bloggers, Jae Lee and Pantelis ...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential viral genes. How it...blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats (LTRs) that facilitate...Bernaudin F, Girot R, Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...These primers are designed to anneal to sites that flank the insert site. A positive clone will produce a...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...know how it goes! Acknowledgements: I’d like to thank the PTA of Butler Elementary for trusting me with...with Math & Science Night, and a special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    ...in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein...Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips ...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...

  16. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...possible facing the committee and not the whiteboard. Thank everyone for the opportunity to share your research...the opportunity to realize your vision. Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ..., One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder...

  18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...large formats, such as 96-well microplates, and thanks to our dedicated product and development teams,... shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design...
Showing: 181 - 200 of 498 results