Addgene

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Showing: 271 - 300 of 363 results

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Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...expression is activated through the induction of T7 polymerase encoded in the E. coli genome (usually controlled...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...
CRISPR in the Clinic

Type

Blog Post

...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

Type

Blog Post

...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...
Magnetic Control of Proteins: More than a Dream

Type

Blog Post

...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....
3D Printing Meets CRISPR Cas9

Type

Blog Post

...the surface of the protein where the nascent polypeptide emerges from the exit channel. He then said...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...
Plasmids 101: Degron Tags

Type

Blog Post

...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...Chemical transfection Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...Praparaten. Leipzig: S. Hierzel.  J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...
An Introduction to Adenovirus

Type

Blog Post

...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...
Uncovering Ancestral Bias in CRISPR Research

Type

Blog Post

...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...
Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...has four layers of location which can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube...
Deciphering the Mysteries of Behavior with Viral Vectors

Type

Blog Post

... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

..., including investigations into environmental pollutants and health, embryo growth, brain function, and...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...
AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

...Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary...
Career Insights: Technical Support Specialist

Type

Blog Post

...the job. When dealing with customers, you must be polite, friendly, patient, attentive, empathetic, and ...

Showing: 271 - 300 of 363 results

Results per page:

Open Resources and Plasmid Tools For Studying C. elegans

Educational Planarian Tools for Teaching Developmental Biology Techniques

Antibodies 101: Stripping and Reprobing Western Blots

CRISPR in the Clinic

Hot Plasmids and Viral Preps - January 2021

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

Magnetic Control of Proteins: More than a Dream

3D Printing Meets CRISPR Cas9

Fluorescent Tagging of Endogenous Genes with SapTrap

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Viral Vectors 101: Producing Your rAAV

Plasmids 101: Degron Tags

Tips for a 1st time AAV user (by a Rookie AAV user)

Hot Plasmids and Viral Preps - May 2021

Truncated gRNAs for Regulating Gene Expression

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Light Sheet Fluorescence Microscopy

CRISPR 101: Ribonucleoprotein (RNP) Delivery

An Introduction to Adenovirus

Uncovering Ancestral Bias in CRISPR Research

Droplet Digital PCR for AAV Quantitation

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Tips from the Repository Trenches: Using Barcodes to Track Samples

Deciphering the Mysteries of Behavior with Viral Vectors

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

AAV Titers: Where Do They Come From And What Do They Mean?

Career Insights: Technical Support Specialist

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