We narrowed to 386 results for: nans

1. Antibodies 101: ChIP

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   Blog Post

   ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

2. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...

3. Clear and Accessible Writing

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   Blog Post

   ... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...

4. Viral Vectors 101: Viral Vector Elements

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   Blog Post

   ... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...

5. Viral Vectors 101: Types of viruses

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   Blog Post

   ...is not currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition...

6. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes to the...

7. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ...detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog ...

8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...minimized with high powered research designs. Unanticipated discoveries Finally, there are good reasons ...

9. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations...

10. Plasmids 101: Yeast Vectors

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    Blog Post

    ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

11. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1...

12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins should act as ...

13. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ... concentration of all the samples by gel or by nanodrop to assemble the  fragments based on their molar...

14. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...that lacks genetic tools is the bee microbiome. Nancy Moran’s and Jeffrey Barrick’s labs at The University...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...series of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or...

16. Celebrating 15 Years of Scientific Sharing

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    Blog Post

    ...about the stewardship of this in the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board...

17. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model first removed...

18. Summer SciComm: Preprints

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    Blog Post

    ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

19. What's New in CRISPR - November 2021

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    Blog Post

    ... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...

20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...against mCherry, and secondary antibodies coupled to nanogold particles, that allow a high-spatial resolution...

21. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...Other information may also be critical to the maintenance and use of certain plasmids, such as special ...

22. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post

    ... mutants do not cause off-target effects at unanticipated sites and, as predicted, decrease off-target...

23. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    ...want to program a cell to identify and target a malignant cell, then you’d need the ability to turn on genes...

24. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post

    ...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...

25. Working with Nuclear Receptors

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    Blog Post

    ...of all organisms depends on efficient energy maintenance - through acquisition, storage, and utilization...

26. Protocol - How to Ligate Plasmid DNA

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    Protocol

    ...Information The final step in the construction of a recombinant plasmid is connecting the insert DNA (gene or...

27. Immunocytochemistry

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    Protocol

    ...Blog Posts Antibody Guide Western Blot Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry...

28. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham, R., Benito, R., Boeke, J.D., Bussey, H. et al...

29. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    ...properties and can be used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime...

30. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol...