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  2. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ... a team. As described here in a 2012 Intuit blog post, a study coming out of MITs Human Dynamics Laboratory...effective delegation will be covered in a future post in this series). Members carry on back-channel...

  3. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding your most used protocols & references...wild with sticky notes and labels. Color code your Post-its and write brief descriptions on each. Place ...

  4. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...significant pairings, additional tests, referred to as post-hoc analysis, must be performed. These will be discussed... choosing an ANOVA/MANOVA statistical test   Post hoc tests Knowing that significance exists somewhere... multiple groups is rarely enough information. A post hoc test, done after your initial analysis can provide...ANOVAs/ANCOVASs must be performed first, and then post hoc tests can be run on these tests. All sample...

  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...This post was contributed by guest blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics ...researcher in the Lu Lab at MIT. Note: The following blog post reduces the content of our paper, “Synthetic recombinase-based...they can control recombinase activity. For example, post-transcriptional recombinase activation via RNA toehold...Student to SynBio Entrepeneur  Read All of Our SynBio Posts Additional Resources on Addgene.org Visit Our ...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning Qin and... this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered Mice and Part 2:...50% when using ESC (Cohen, 2016). See Our Recent Post for Tips on CRISPR Gene Editing in Mice Disadvantages...additional advice from John Doench’s gRNA design blog post. Finally, always remember that you are working with...

  8. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ... of another academic year, graduate students and post-docs alike are wondering where the time has gone....2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong Science...planning help alleviate the anxiety of graduate and postdoc training. On the other side, the PI has an opportunity...

  9. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...cleavage sites should one need to remove the tag post-purification. A sample of pCri constructs: pCri...to glycosylate proteins and may recapitulate the post-translational modifications added to proteins in...

  11. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ... for fun and life outside of the lab? This final post in the PI Interview Series investigates how these...Maintain a Good Work-Life Balance? Read the Previous Posts in this Series Advice for Choosing a Research Project...

  12. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...expression in transduced cells whenever possible. This post will describe our workflow for viral genome sequencing...quality control process! The images in this blog post were created using Snapgene and Geneious software...Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS for Plasmid Quality Control...

  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...vectors? Browse our Viral Vectors 101 blog posts Read blog posts about neuroscience Resources on addgene.org...increased potency. Download our chemogenetics poster! This blog post was originally written by Maya Peters Kostman...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...pyrimidine bases (C and T). (Check out our previous post on these editors for more details on how they work...problematic in the development of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a ...story still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion...Glossary We introduced a lot of terminology in this post! Here’s a quick refresher if you ever lose track... glycosylation to create an abasic site. In this post, it refers to the same approach as a CGBE. BER...

  15. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...which contains the viral genome. In today’s blog post, we’ll focus on the packaging plasmid and take a...understanding of the AAV RepCap plasmid! Check out this blog post to learn more about the AAV transfer plasmid! ...

  18. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common terminology for...guide or our CRISPR 101 ebook. We also have a blog post on the structural biology of Cas9. Want a simpler...spacer sequence is the RNA guide, like a wanted poster that Cas9 uses to track down its suspect—the target...

  19. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...needs. If you need an FP refresher, see our FP blog posts.     References Sattarzadeh A, Saberianfar R, Zipfel...Fluorescent Protein Plasmids and Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent...

  20. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...about being an effective manager, read the other posts in this series. Listen to our podcast interview ... on this topic and get the whole series of blog posts in one pdf - download the Addgene Management for...

  22. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic...reproduce the research that produced the data. In this post I’d like to delve deeper and look specifically at... Minisymposium on Reproducibility Read all blog posts about scientific sharing Resources on Addgene.org...

  24. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    .... To see even more tools, head over to this blog post that highlights five different methods to use CRISPR...behind-the-scenes glimpse of their work, head over to their blog post. Months later, we partnered with Ginkgo Bioworks... remote collaborations Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources...

  25. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...optional media exchange can be performed 12–18 hours post-transfection to help remove toxic transfection reagents...uses, check out our ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based...applications by reading our Viral Vectors 101 series!  This post was originally written by Didem Goz Ayturk and updated...

  26. Viral Production at Addgene

    Type
    Blog Post
    ...catalogue of viral preps available to request. In this post, we’ll walk you through how we produce both our ... function. These data are sometimes reported or posted on the material page for the corresponding catalog... of early passage cells. Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma...

  27. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...prescription for a bacterial infection. While this post will, indeed, focus on bacteria, it is important...bacterium to survive). Check out our recent blog post on CRISPR antimicrobials to learn how scientists...

  28. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...their knowledge to a variety of problems. In this post, I’ll share what I’ve learned as a healthcare consultant...and occasionally being faced with “shifting goal posts” as a part of a case, consulting can sometimes be...

  29. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... View Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...experimentally confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence...

  30. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... Resources Read other posts about life at Addgene!Read more of our careers posts!    ...successful event at your company. The point of this post isn’t to make you all jealous that you don’t work...Spread word of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget...
Showing: 271 - 300 of 782 results