Addgene

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Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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Blog Post

...while also updating brand messaging, as well as simplifying the page, and making it all look clean and easy...
What's New in CRISPR - September 2019

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...also developed one of these systems further for simultaneous gene activation and editing. Read the paper...
A Better Way to Get Customer Support: The Help Center

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Blog Post

...Center’s content from wherever you are on our site. Simply click on the icon, search for answers, and choose...
Embracing Serendipity: A Crucial Element in the PhD Journey

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Blog Post

...serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments. Widening...
Antibodies 101: Fc Effector Functions

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...large parasite, ADCC can be induced through a very similar interaction between IgE antibodies and eosinophils...
CRISPR 101: Drug discovery and CRISPR

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... all diseases are fully characterized or have a simple pathology. In these cases, preliminary identification...
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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Blog Post

...Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save any image...
New CRISPR Web Resources and #12DaysofCRISPR Recap

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Blog Post

...only the specified RNA target. Bonus: It displays similar on-target activity to siRNA, but with far fewer...
Oh, The Places You Can Go: Careers in Science Communication - Product Development

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Blog Post

... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science...
Some Like it Hot: Thermostable GeoCas9

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...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...
Bricking Science: Portraying Scientific Reality Through LEGO

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Blog Post

...faced an odd situation with experiments? Our art simply mirrors our lives at this point! It was a great...
Running for Rare Disease, Running for FOP, Running for AJ

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... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...
Bringing Sustainable Practices to the Lab: Recycling

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Blog Post

...prepaid return shipping label. To return the box, simply peel your address sticker off the box, remove the...
Hot Plasmids and Viral Preps - July 2021

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Blog Post

...CRISPR-Act3.0, developed by Yiping Qi's lab, allows simultaneous gene activation in plants. FnCas12a, unlike...
Understanding GPCR Signaling Using TRUPATH

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Blog Post

...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer (FRET), in...
Plasmids 101: What is a plasmid?

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...and troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a ...
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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...drug, disease, or condition of interest using a simple keyword search. For example, searching the term...
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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Blog Post

...engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect observed in bacteria...
SunTag and Fluorescent Imaging

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Blog Post

...chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking Caveats ...
Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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...that have lost the sacB gene. SacB can be used similarly to CcdB. For example, in the gene expression plasmid...
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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Blog Post

...extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this month's Addgene...
10 Ways to Share Your Science!

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...Natural Sciences and the Morehead Planetarium. Similar things are likely happening in your community, ...
Advice for Choosing a Research Project

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...of your career by connecting you with people of similar interests and goals. The following questions can...
CRISPR/Cas9 FAQs Answered!

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Blog Post

...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time...
Plasmids 101: Antibiotic Resistance Genes

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...Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find...
History of CRISPR Cas - A tale of survival and evolution

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Blog Post

...polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system that scientists...
New Viral Vectors - Winter 2025

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Blog Post

...pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2...
Summer SciComm: Science Storytelling

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Blog Post

... big-picture problem, by asking your audience a simple question, like “Have you ever thought about how...
In Vivo Biotinylation of Bacterial Fusion Proteins

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Blog Post

... protein (MBP, New England BioLabs). MBP has a similar function as GST in that it provides a method to...
The Strength of Story Telling

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...which makes honing your ability to tell stories a similarly crucial skill for career advancement. Scientists...
Science Careers: Unruly Interests Feed Many Paths

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Blog Post

...career Developmental biology caught me early on: a simple egg is packed with information. I am fascinated...
Using Addgene's New Viral Service for Your Research

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Blog Post

... phenotype of interest. Optogenetic tools for similarly controlling neuronal activity with light will ...
Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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...the stochastic expression of XFPs is possible by simply introducing a mixture of XFP genes. Stochastic ...
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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...reference sequence database. Through a series of simulations and machine learning, they created a classifier...
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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...reporter. Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive...
Plasmids 101: Origin of Replication

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Blog Post

... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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...Wanisch Listen to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group...
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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... compared to the original AraC system, BLADE is simply more precise. “We show that you have less heterogeneity...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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...IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans...
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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Blog Post

...expected locations. An analysis of mWasabi gave similar results. We hope this blog post helped you see ...
Intro to Our Summer SciComm Series!

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...communicating science to laypeople. Laypeople are simply non-experts in the field of science you are communicating...
Addgene Begins Distribution of Recombinant Antibodies

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....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...
Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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...plasmid, whereas the round tan colonies that look similar to normal E. coli cultures contain the recombined...
Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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...The data are clear - the SEC-based system is both simple to use and highly efficient for C. elegans genome...
Another Pathway into Cells: iTOP

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...instead of supplying a DNA construct, they could simply induce cells to take up protein directly? It’s ...
K. phaffii: Rising to the Occasion

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...evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...
Summer SciComm Series: A PhD in Science Communication

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Blog Post

...adeolitis” – received the best ratings overall, simply because none of the participants had preconceived...
Learning Technical Skills at a Lab Central Workshop

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...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out...
Cultivating Community Science at BosLab

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...protocols so that anyone can engineer yeast to do similar things. The focus is to make yeast engineering ...
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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Blog Post

...or in cell nuclei. The two photosensitizers had similar rates of off-target damage to neighboring cells...

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