We narrowed to 430 results for: ERG
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TypeBlog Post...positioned PAM without undesired bases nearby that may undergo unwanted editing. Just like with base editing, ... and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, generally the strongest...
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Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal...PMC4028491. Van Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...find ways to tag and curate reagents from these emerging organisms so scientists can easily find the useful...electric fish are excellent systems to study for convergent evolution because electric organs arose in different... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...https://doi.org/10.1126/science.1231143 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung....2014.02.001 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...symptoms of the disease. However, there are some emerging treatments that have shown some positive potential...as these new detection techniques and therapies emerge into mainstream use, they will have a much greater... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post... work she put into this. 5. Reddit forums are evergreen! With all the great questions that were posted...you’ll see below, it pretty nicely displays her energetic, truthful and helpful style: Joanne also provided... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...PMC3775282. 2. McDevitt, Ross A., et al. "Serotonergic versus nonserotonergic dorsal raphe projection neurons: ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...generate a specific sequence change the cell must undergo homology-directed repair (HDR), a distinct cellular...developed a technology called CORRECT, which, by undergoing two rounds of gene-editing enables scarless introduction... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...sucrose into a toxic metabolite. Cells that have undergone a double crossover will not contain sacB, allowing...are those where the allelic exchange vector has undergone single crossover and integrated into the genome... -
Five Popular Model Organisms
TypeBlog Post...genetics, and the nervous system. The fly has also emerged as an important human disease model with potential...and read about new systems being developed for emerging model organisms! References Brenner, Sydney. ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...RFP. Cells that express the construct but do not undergo recombination can be detected by immunostaining...background, each cell in the mouse that expresses Cre undergoes exactly one recombination event, leading to each... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic...2020.04.20.052159 Zhang, F., Abudayyeh, O. O. & Gootenberg, J. S. A protocol for detection of COVID-19 using... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...downstream of the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002). How ...Wassmer SJ, Luna-Velez MV, Xiao R, Bennett J, Vandenberghe LH (2017) Evaluating Efficiencies of Dual AAV... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include... 25337688. PubMed Central PMCID: PMC4244833. Kellenberger, Colleen A., et al. "RNA-based fluorescent biosensors... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ. In vivo gene editing...PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...What is the energy level in the lab? Does a frenetic atmosphere make you edgy or energized? Are you ... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post... to tell if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA... The Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... (2006): 687-699. PubMed PMID: 16464020. 3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ... PMID: 17466627. 25. Luo, Jianying, Donald E. Bergstrom, and Francis Barany. "Improving the fidelity of... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...In the 1960s, Har Gobind Khorana, Marshall W. Nirenberg, and Robert W. Holley cracked the genetic code... a renaissance in the field of health sciences, energy, and agriculture, its portrayal in popular fiction...