We narrowed to 438 results for: ERG
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TypeBlog Post... work she put into this. 5. Reddit forums are evergreen! With all the great questions that were posted...you’ll see below, it pretty nicely displays her energetic, truthful and helpful style: Joanne also provided...
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Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...PMC3775282. 2. McDevitt, Ross A., et al. "Serotonergic versus nonserotonergic dorsal raphe projection neurons: ... -
COVID-19 Resources
TypeCollection...Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N. H., Nitsche, A., ... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...downstream of the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002). How ...Wassmer SJ, Luna-Velez MV, Xiao R, Bennett J, Vandenberghe LH (2017) Evaluating Efficiencies of Dual AAV... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic...2020.04.20.052159 Zhang, F., Abudayyeh, O. O. & Gootenberg, J. S. A protocol for detection of COVID-19 using... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...sucrose into a toxic metabolite. Cells that have undergone a double crossover will not contain sacB, allowing...are those where the allelic exchange vector has undergone single crossover and integrated into the genome... -
Five Popular Model Organisms
TypeBlog Post...genetics, and the nervous system. The fly has also emerged as an important human disease model with potential...and read about new systems being developed for emerging model organisms! References Brenner, Sydney. ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...RFP. Cells that express the construct but do not undergo recombination can be detected by immunostaining...background, each cell in the mouse that expresses Cre undergoes exactly one recombination event, leading to each... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...generate a specific sequence change the cell must undergo homology-directed repair (HDR), a distinct cellular...developed a technology called CORRECT, which, by undergoing two rounds of gene-editing enables scarless introduction... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...messenger cyclic di-AMP in Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include... 25337688. PubMed Central PMCID: PMC4244833. Kellenberger, Colleen A., et al. "RNA-based fluorescent biosensors... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ. In vivo gene editing...PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...What is the energy level in the lab? Does a frenetic atmosphere make you edgy or energized? Are you ... -
9 tips for a successful postdoctoral experience
TypeBlog Post...learning. Serve as a daily mentor for a summer undergrad, rotating grad student or high school student.... the time to develop strategies to rest and re-energize yourself, and to address all of the other parts... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... (2006): 687-699. PubMed PMID: 16464020. 3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ... PMID: 17466627. 25. Luo, Jianying, Donald E. Bergstrom, and Francis Barany. "Improving the fidelity of... -
Antibodies 101: Validation
TypeBlog Post...Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post... to tell if the dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA... The Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...In the 1960s, Har Gobind Khorana, Marshall W. Nirenberg, and Robert W. Holley cracked the genetic code... a renaissance in the field of health sciences, energy, and agriculture, its portrayal in popular fiction... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...vector allows you to visualize which cells have undergone recombination. Reporter plasmids can also be useful... -
CRISPR References and Information
TypeCollection...at the German Cancer Research Center (DKFZ) in Heidelberg. CRISPR FAQ forum (Link opens in a new window...