We narrowed to 225 results for: GFP

An introduction to the use of antibodies in immunoprecipitation, including antibody immobilization, controls, and a basic overview of techniques.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

Chromoproteins are a subset of the fluorescent protein family. They give strong color in visible light and can be used for molecular biology experiments.

Find tips for transforming arabidopsis with your plasmid construct of interest.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

Fluorescent proteins can mis-fold or form aggregates outside of the cytoplasm leading to errors in localization. Avoid these issues with new mOX-FPs.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Addgene's viral vector service has grown much since it was first launched in 2016! Read about our service and the unique challenges it presents in this blog post.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

Check out all of the plasmids featured in our hot plasmids articles from 2016.