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...et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

...success of BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the...requires that you empirically test a number of mutants to identify those that inhibit BiFC in your cellular...

... but won’t tell you if your products have any mutations.  There are lots of different cloning strategies...expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive...

...the deletion reduces the risk of insertional mutagenesis by limiting the interaction between viral LTR...potential for oncogenesis through insertional mutagenesis. These risks are largely based upon the vector...

... HEPN domains in other proteins. As with Cas9, mutating key residues in the Cas13a molecule results in...Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic DNA, and detect COVID...

...methods were available to create targeted gene mutations in anoles lizards or in any reptiles at all. But...was able to make an albino lizard with targeted mutations. Now what? What would you study first? They are...

...determine the presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate...SHERLOCK could also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related...

...HaloTag, Figure 4), they determined that an A122D mutation in the rhodopsin domain conferred several new ...potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also...

... the sequence, normally through the synonymous mutation of a codon. You can domesticate the sites yourself...colony PCR electrophoresis), is free of undesired mutations, and that you have the correct four-base overhangs...

...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...

...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...base editing , or prime editing experiments, most mutated alleles will not contain the desired edit. Even...

...mEGFP. This variant was made by introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP...with improved brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly ...

...biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein homologues...

...2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas...Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922...

... can mediate recombination.) The deletions are mutually exclusive, as either deletion removes one of the...following the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can be detected...

...Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre None ...-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO EYFP...Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55650 pAAV-hSyn Con/Fon EYFP...recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137119 pAAV-EF1a-Con/Fon-...Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55645 pAAV-hSyn Con/Fon hChR2...Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137148 pAAV-nEF-Con/Fon-Arch3.3...recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 177669 pAAV-nEF-Coff/Fon ...

... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...

...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):...

...active area of research due to the possibility that mutation or recombination with human retroviruses could...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human...

...with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...research to search committees, and can lead to mutually beneficial collaborations. 7. You cannot get too...