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We narrowed to 402 results for: Mut

Showing: 181 - 200 of 402 results

  1. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  This process...For example, multiple studies show that certain mutations of the BAP1 gene play a role in susceptibility...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... HEPN domains in other proteins. As with Cas9, mutating key residues in the Cas13a molecule results in...Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic DNA, and detect COVID...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...HaloTag, Figure 4), they determined that an A122D mutation in the rhodopsin domain conferred several new ...potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...determine the presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate...SHERLOCK could also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...mEGFP. This variant was made by introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP...with improved brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly ...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas...Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein homologues...

  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...

  11. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...research to search committees, and can lead to mutually beneficial collaborations. 7. You cannot get too...

  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...active area of research due to the possibility that mutation or recombination with human retroviruses could...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...

  20. CRISPR Cheat Sheet

    Type
    Blog Post
    ...multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ...
Showing: 181 - 200 of 402 results