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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 181 - 200 of 416 results

  1. Deep Dive: qPCR

    Type
    Blog Post
    ...you run the plate.    Analyzing There are a number of methods available for analyzing qPCR data, most...Shaun S. LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... effective at generating plasmids with varying numbers of repeated sequences or inserts, which can be ...authors optimized these systems by: 1) decreasing the number of plasmids required for each input/output system...new origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the ...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    ...to your application — and double-check that lot number while you’re at it! Polyclonal or monoclonal Both...you are running a denaturing blot.  There are a number of lysing methods and buffers to choose from. If...

  10. Editor's Choice, October 2016

    Type
    Blog Post
    ...downstream effects including possibly decreasing the number of postdocs various academic institutions can afford...

  14. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...symptoms. The World Health Organization says the number of new cases could rise to 10,000 a week within...

  15. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold and >3,800...

  20. Reflections and Looking Ahead

    Type
    Blog Post
    ...commitment to open science and shared progress. But numbers tell only part of the story. This year, we made...
Showing: 181 - 200 of 416 results