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We narrowed to 420 results for: Numb

Showing: 181 - 200 of 420 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... effective at generating plasmids with varying numbers of repeated sequences or inserts, which can be ...authors optimized these systems by: 1) decreasing the number of plasmids required for each input/output system...new origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the ...

  3. Technical Design of a Western Blot

    Type
    Blog Post
    ...to your application — and double-check that lot number while you’re at it! Polyclonal or monoclonal Both...you are running a denaturing blot.  There are a number of lysing methods and buffers to choose from. If...

  4. Antibody Guide

    Type
    Guide
    ...valency, or number of binding sites available per antibody unit, which is typically the number of antibodies...while the number of proteins that you can detect in a single assay is limited by the number of signaling...therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. Check for and...antibody, which becomes the limiting factor for the number of proteins you can detect in a single assay. Overview...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...denoted using two numbers separated by a slash (e.g. AAV2/5), where the first number indicates the serotype...far been identified, and it is likely that this number will continue to increase. This diversity makes...serotype of the genome and the second number the serotype of the capsid. Pseudotyped vectors can improve... BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that can target whole ...

  6. Guide to Using Pooled Libraries

    Type
    Guide
    ... the number of plasmids present in the library - for example, the Moffat lab uses 200x the number of cells...cells as plasmids in the TKO library. Using large numbers of cells minimizes the effects of random chance...

  7. Adenovirus Guide

    Type
    Guide
    ...denoted using two numbers separated by a slash (e.g. Ad5/35), where the first number indicates the serotype...serotype of the genome and the second number the serotype of the capsid. Use of these pseudotyped vectors...

  13. Editor's Choice, October 2016

    Type
    Blog Post
    ...downstream effects including possibly decreasing the number of postdocs various academic institutions can afford...

  17. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...symptoms. The World Health Organization says the number of new cases could rise to 10,000 a week within...

  18. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold and >3,800...
Showing: 181 - 200 of 420 results