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...to your application — and double-check that lot number while you’re at it! Polyclonal or monoclonal Both...you are running a denaturing blot.  There are a number of lysing methods and buffers to choose from. If...

...valency, or number of binding sites available per antibody unit, which is typically the number of antibodies...while the number of proteins that you can detect in a single assay is limited by the number of signaling...therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. Check for and...antibody, which becomes the limiting factor for the number of proteins you can detect in a single assay. Overview...

...denoted using two numbers separated by a slash (e.g. AAV2/5), where the first number indicates the serotype...far been identified, and it is likely that this number will continue to increase. This diversity makes...serotype of the genome and the second number the serotype of the capsid. Pseudotyped vectors can improve... BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that can target whole ...

... the number of plasmids present in the library — for example, the Moffat lab uses 200x the number of cells...cells as plasmids in the TKO library. Using large numbers of cells minimizes the effects of random chance...

... on the addgene.org homepage! You can track the number of plasmids right below the search bar and more...

...denoted using two numbers separated by a slash (e.g. Ad5/35), where the first number indicates the serotype...serotype of the genome and the second number the serotype of the capsid. Use of these pseudotyped vectors...

...open-source distributors such as Addgene, as well as a number of online user forums such as http://www.genome-engineering.org...

...adjustments for the type of tissue culture dish and number of dishes used. Here’s to making AAV packaging ...

...plasmid sharing over the years, we crunched the numbers (shout out to Addgenie Priyanka Ramanathan for ...

...Allied Sciences in Ghana. He’s done research at a number of institutions in Ghana and most recently got ...

...downstream effects including possibly decreasing the number of postdocs various academic institutions can afford...

...decide what lab to join? 11:25 - 11:50 : What is the number one question you think rotation students should...

...amino acid immediately followed by its position (number) relative to the start Methionine (Met) followed...

...progress their research. *2015 total reflects the number of distributed plasmids as of March 26, 2015. How...

...symptoms. The World Health Organization says the number of new cases could rise to 10,000 a week within...

...mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold and >3,800...

...potential and the promise of science to help a great number of people. I look forward to working with Addgene...

...epitope tags like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially...

...realize its potential as a research tool. Soon, the number of people studying it grew. In particular, studies...

...possible to quickly and efficiently assemble a large number of building blocks with just two or three inexpensive...