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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 497 results for: RAP

Showing: 181 - 200 of 497 results

  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe.2000.0116...Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org/10.3390/v12091016...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative...25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic of green fluorescent protein...PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...pitch that requires reading two pages or even a paragraph to get to the punch line is a terrible sales pitch...conviction that I had a flair for communication was rapidly dispelled. She would ask me, "Is this what you ...that we can manipulate to study pathology and therapies." This is only the first few minutes of the...

  4. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... decomposition analysis is performed yielding a graph of edits in the input population or clone.   ...sgRNA sequence. The TIDE software will generate a graph representing all insertions and deletions identified...MC, Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...are easy to see (and for heavens sakes, label all graphs and data professionally). Do remember the fine ... use no notes and can concentrate on delivery, rapport with the audience, and their own excitement about...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...Regions of the Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j.ymthe.2004.05.024...Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org/10.1089/hum.2019.277...Peripheral Nervous System and Relevance to Human Gene Therapy. Front Mol Neurosci 13. https://doi.org/10.3389...

  7. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...the culture. This system is now used as it shows rapid gene expression kinetics as compared to the Tet-...Regulatable Lentiviral Vector System, Viral Gene Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech...(2000): 249-71. PubMed PMID: 10983732. 5. Gene Therapy Net Lentiviral Vectors Page Additional Resources...

  8. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...translational researchers begin to use CRISPR as a therapy. Low frequency off-target effects may generate ...play significantly into the realm of clinical therapeutic development as CRISPR is used not only to study...CRISPR/Cas9, and megaTAL nucleases." Molecular Therapy (2015). PubMed PMID: 26502778. 13. Frock, Richard...

  9. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently become...Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural...Tags Fluorescent tagging of endogenous genes with SapTrap Resources on Addgene.org Browse CRISPR plasmids...

  10. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...University. No matter whether you are a sports photographer at the Super Bowl, a medical technologist taking...just those in the focal volume. This leads to more rapid photobleaching and a reduction in signal intensity... 2-photon microscopy, lightsheet microscopy is rapidly becoming the preferred technique for many of these...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...2014.   In vivo uses For both research and therapeutic purposes, antibodies can be used to affect cellular...functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies can bind to and inhibit...investigating signaling pathways or developing therapeutics. Experimentally, antibodies conjugated to fluorescent...

  13. Deep Dive: qPCR

    Type
    Blog Post
    ...probe into a single molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces...chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr ...brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely...

  14. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...advancing this field and how we are able to support rapid progress in all areas of research. We welcome your...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...memory, a special type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional...could also be powerful tools for the field of gene therapy if they can reach the affected part of the brain...Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic Injection...

  16. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ... (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid sharing over...and become available into 2021. Check out the infographic below for some Addgene depositor fun facts for...

  17. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...students. As part of this discussion, we mention graphs such as the one shown to the right that illustrate...but many students discuss the third year dip (see graph). Niroshi and Ben give their insights as to what...

  19. Editor's Choice, September 2016

    Type
    Blog Post
    ...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...That’s it for now. As I indicated in the opening paragraph, I’m currently on my way back from a very successful...
Showing: 181 - 200 of 497 results