...biological studies, including investigations into environmental pollutants and health, embryo growth, brain ...example, specific tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers... for neuroscience investigations. For example, neuronal activity can be tracked in whole brains of swimming...high-resolution mapping of the brain by uniquely labeling neurons. Zebrafish are also amenable to manipulation of...consequences of activating or silencing specific neurons in living larvae. In addition, zebrafish have been...PubMed PMID: 18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely...

...make them ideal for studying in a laboratory environment. We’ve covered five model organisms in a previous...candidate for introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia... disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical...genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant...Regeneration and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159/000323761...the Axolotl Model for Regeneration and Aging. Gerontology, 66(3), 212–222. https://doi.org/10.1159/000504294...

...Access to Projection Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters..., 2023). It may be a good choice for targeting neurons, with high transduction frequencies in the central...to target the central nervous system, including neurons, AAV5 is generally the best choice for targeting... is derived from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up ...

...molecule at all, leading to inconclusive results, erroneous conclusions, and wasted time. In fact, many have...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018; Uhlen, et...that antibody works as expected, you will see a strong antibody signal in the retinal sample, but little... your antibody (B). Your results show a lovely, strong band from the cells transfected with your protein...Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL... Ashley Waldron...

...mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse neuron without...optogenetic silencer in experiments with mouse cortical neurons, far out-performing previous attempts to engineer...of HcKCR1-EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential frequencies with ...antibody anti-c-Myc [9E10] now available! by: Ashley Waldron The majority of Addgene's more than 50 antibodies...

... Frank Graham showed HEKs display many immature neuron-like qualities, which is inconsistent with kidney...is next to the kidney and is known to have some neuronal qualities.) While HEKs probably aren’t a great...researchers using the cell line. What the HEK is wrong with it?   All cultured human cell lines are susceptible..., et al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of ...Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389...

...at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent problems...smaller plasmids are formed. Viral vectors are prone to recombination In lentiviral vectors, recombination... elements. Knowing how to amplify recombination-prone plasmids and testing your prep can save you the ...H (2012) Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3...Genetic recombination of bacterial plasmid DNA: electron microscopic analysis of in vitro intramolecular...

...equipment always helps. But how does one build a strong collaborative research team motivated by similar...your lab team and foster an effective working environment? Tom Ellis was offered a start-up package that...ways to improve the lab. “An effective working environment for research is totally dependent on communication...while others are more productive in a handsoff environment. “Treating people fairly does not mean equally...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

...plasmid for expression in forebrain GABA-ergic interneurons (83895). See our Serotype Testing AAV inventory...with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics lie in...system that allows for specific manipulation of neurons. We have expanded our AAV collection to include...targets expression of ChR2 to forebrain GAB-ergic interneurons through the mDlx enhancer element. Plasmid...DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361...

...error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error prone PCR,...bacterial growth conditions. Expression of an error prone polymerase I (PolI3M) is capable of mutagenesis ...PCR, EvolvR uses an error prone polymerase to introduce mutations. However, by using nCas9 to direct PolI3m...   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused to a nicking Cas9...

...signaling pathways in specific cell types (typically neurons). While all of these viruses are popular, pAAV-...pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes et al..... The human synapsin promoter further specifies neuron-specific expression of the DREADD (Kügler et al...Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding behavior in mice. J Clin Invest..... Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an...

...skills in strong relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...software product management was either. Coming from a strong background of undergraduate research straight to...energized from interacting with others, and have strong communication skills to tailor your message to ...implementation review. The developers discuss which front-end and back-end coding frameworks to use. I mostly...

...29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids...have been successfully transdifferentiated into neurons and β-cells [40-42]. Reprogramming and transdifferentiation...S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods Mol ...human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p...pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID: 25782665...Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010. 463(7284): p....

...increased iron serum levels and early-onset cataracts, is characterized by point mutations in the Iron Response....  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when...and IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, ... loss of binding correlated with increased serum iron levels seen in HHCS patients. These are just three...

...somewhat similar to an academic research environment” with a strong focus on publishing, but still made it...initial resistant populations can be eradicated up-front quickly. This approach is in contrast to the current...at cancer cell resistance to ABL1 inhibitors in chronic myeloid leukemia. Bhang says the work highlighted...2015): 440-448. Pubmed PMID: 25849130. 2. Hata, Aaron N., et al. "Tumor cells can follow distinct evolutionary...

... an extensive infrastructure of molecular and electronic companion technologies that has transformed the...CRISPR/Cas9-mediated genotyping, as well as our electronic reader that provides portable quantification ...Leslie Dan Faculty of Pharmacy at the University of Toronto. His research is focused on developing cell-free...biology applications for human health and the environment.     Alexander A. Green is an Assistant professor...4. Messina, Jane P., et al. "Mapping global environmental suitability for Zika virus." Elife 5 (2016):...

...9514755. Richard Gronostajski: NFI-related diseases of neural development Richard Gronostajski is a professor...Paula Videira, Michael McMurray, and Richard Gronostajski who each are being awarded free plasmids form...they affect brain and muscle development. The Gronostajski lab has generated mice wherein it's possible...Campus in Aurora, Colorado, USA.     Richard Gronostajski is a professor at the University of Buffalo....

...could ever do! However, there is quite a bit of upfront research you have to do to find said robot and ...into the robotics spirit include: Start using electronic multichannel and single channel pipettes. These...help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table top device ...has a roller to go over caps and push them into micronic tubes instead of pushing them in one by one. It... It seems very simplistic and it isn’t electronic but it is one of the most helpful machines I have used...

...antibody is produced from a new set of animals), and wrong application (different experiments/conditions can...that this antibody has specific binding and has a strong signal. We call the unique pattern of the antibody...7s) then the signal is weak and vice versa for a strong signal. However, exposure time and its relationship...compare exposure times of our samples. A prominent, strong signal has a shorter exposure time than the negative... neuroanatomy without the fear of referencing a wrong section. The antibody screening and development ...

...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing ...way to guide inheritance that might be less error prone than DNA replication. He too mentioned how “surprising...regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University then spoke about using...treatment resulting in major problems in hospitals. Dr. Qimron proposes using bacterial phage vectors with CRISPR...