... from the Structural Genomics Consortium (SGC) Toronto collection...Genomics Consortium Plasmids Browse SGC Toronto collection Structural Genomics Consortium Plasmids Structural...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags ...

...Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal Tracing These... tracing from single, genetically targeted neurons. Neuron. 53(5):639-47. PMID: 17329205 Don’t See What...service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus...with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies...rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...that it could only infect a genetically specified neuronal population. This is done using the envelope protein... pseudotyping the rabies virus with EnvA, only neuronal cells engineered to express TVA will be infected...

...University Stephen Sligar , University of Illinois Ron Vale , University of California, San Francisco Browse...

...Jan-Willem Veening Christopher Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering...plasmids related to intercellular signaling and environmental sensing. Strains Browse Addgene's collection...

...hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C ...TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4: hTLS-CHOP.pBSKS(+) FUS T7 ALS David Ron 21831 TLS 5:hTLS-CHOP....Friedreich ataxia Michael Ristow 15239 pWBC KIF5A T7 ALS Ron Vale 15402 pBlue-hFX(AA88)-HA FXN HA, HIS, AU1 T7...hTLS-CHOP.pCDNA1 FUS CMV ALS David Ron 22106 pWJPrP5 PRNP Dementia Susan Lindquist 22109 pWJPrP1 PRNP Dementia...Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia Ronald Kahn 11487...Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald Kahn 11505 pBabe...telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14666 pQE9-His-p97...

...redox. J Cell Biol. 2013 Apr 15;201(2):337-49. David Ron Redox/ROS Thiol redox-sensitive ratiometric sensor...Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine...electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron. 2012 Sep 6;75(5):779-...and cellular processes, or other factors like environmental conditions. With proper experimental design,... fluorescence properties can be affected by environmental factors and the sensor behavior may differ from...High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300...

...CARTPT pEMS1492 intron-lacZ/NLS Ple22 CCKBR pEMS1493 intron-lacZ/NLS Ple23 CCKBR pEMS1494 intron-lacZ/NLS Ple24...CCKBR pEMS1495 intron-lacZ/NLS Ple25 CCKBR pEMS1496 intron-lacZ/NLS Ple26 CCL27 pEMS1497 intron-lacZ/NLS Ple27...CCL27 pEMS1498 intron-lacZ/NLS Ple28 CCL27 pEMS1499 intron-lacZ/NLS Ple29 CCL27 pEMS1500 intron-lacZ/NLS Ple30...CLDN5 pEMS1504 intron-lacZ/NLS Ple34 CLDN5 pEMS1505 intron-lacZ/NLS Ple35 CLDN5 pEMS1506 intron-lacZ/NLS Ple36...ICMT pEMS1593 intron-lacZ/NLS Ple123 ICMT pEMS1594 intron-lacZ/NLS Ple124 ICMT pEMS1595 intron-lacZ/NLS Ple125... pEMS1597 intron-lacZ/NLS Ple129 MKI67 pEMS1600 intron-lacZ/NLS Ple131 MKI67 pEMS1602 intron-lacZ/NLS ...pEMS1650 intron-lacZ/NLS Ple179 RLBP1L2 pEMS1651 intron-lacZ/NLS Ple180 RLBP1L2 pEMS1652 intron-lacZ/NLS...

.... AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core...recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...

...-specific labeling. Neuron. 2021 May 5;109(9):1449-1464.e13. doi: 10.1016/j.neuron.2021.03.011. PMID: ...WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2-WPRE3...WPRE3-BGHpA AiP1691 AiE2366m SYFP2 Lamp5 interneurons Isocortex 220737 pAAV-AiE2366m-minBG-iCre(R297T)...R297T)-BGHpA AiP20068 AiE2366m Cre Lamp5 interneurons Isocortex 224015 pAAV-AiE0724m-minBG-SYFP2-WPRE3-BGHpA...BGHpA AiP12863 AiE0724m SYFP2 Lamp5 interneurons Isocortex 224051 pAAV-AiE0724m-minBG-iCre(R297T)-BGHpA...BGHpA AiP14668 AiE0724m Cre Lamp5 interneurons Isocortex 214480 pAAV-AiE2103m-minBG-SYFP2-WPRE3-BGHpA AiP1287...

...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...Minicircle Human Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, ...functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS One. 2011;6(12):e28719...Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons by defined ...Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl...Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons. Nature....cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...

...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting.... AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core...Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825...Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496...separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857-PHPeB AiP14361...jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru 214856...jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru 214853...

...IFNA10 interferon, alpha 10 MGC119878, MGC119879 IFNA13 interferon, alpha 13 - IFNA14 interferon, alpha...IFNA16 interferon, alpha 16 - IFNA17 interferon, alpha 17 IFNA, INFA, LEIF2C1 IFNA2 interferon, alpha ...MGC142200 IFNA5 interferon, alpha 5 INFA5 IFNA6 interferon, alpha 6 - IFNA7 interferon, alpha 7 IFNA-J IFNA8...

...Bacterial Expression Dronpa3 503 518 19 Monomer Dronpa3-N1 - Mammalian Expression Dronpa3-C1 - Mammalian Expression... 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone to dimerization...35 min Prone to dimerization pcDNA3-mNeptune2-N - Mammalian Expression mNeptune2 600 650 21 Prone to dimerization...Many FPs are monomers at low concentration but are prone to dimerization at high concentration, so this is...Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1 - Mammalian Expression...-C1 - Mammalian Expression Azurite 383 450 14 5 Prone to dimerization pLV-Azurite - Mammalian Lentiviral...-C1 - Mammalian Expression EBFP2 383 448 17 4.5 Prone to dimerization pBad-EBFP2 - Bacterial Expression...

...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...to single-component cellular definitions (e.g. neurons defined by a single gene or projection). Intersectional...Image from Fenno et al., 2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting...and Flp) recognition sequences within synthetic introns. INTRSECT was first shown as a proof-of-concept.... Figure 2: INTRSECT works by inserting short, intronic sequences into the open reading frame (ORF) of...sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase recognition... combinations of recombinases are present, the introns are excised during mRNA processing, producing a...

... for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus detection using CRISPR-Cas13: Open-access...DETECTR to detect coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using... Medicine Coronavirus webpage PLOS Journals COVID-19 Collection Science Journals Coronavirus Collection... for Coronavirus research. Collections... Collections COVID-19 - Plasmids and Resources COVID-19 and Coronavirus Plasmids...knowledge and understanding of COVID-19 and related coronaviruses. To assist with this effort Addgene will maintain... may be of use to scientists. Background The Coronavirus Disease-2019 (COVID-19) pandemic is caused by...

...and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to limb ...progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases include Alzheimer...CRISPR screens to understand more globally how neurons function. They are also delving into new technologies...polyglutamine sequences. These glutamine-rich sequences are prone to misfolding and aggregation and can interfere ...Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive neurological disorder that primarily...the malfunction and death of dopamine-producing neurons in the substantia nigra, one of the movement control...control centers in the brain. The cause of this neuron death is unknown. Despite the idiopathic (having...

...transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd EF-1alpha driven...plasmids along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase...transfer plasmids along with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev...used with 2nd and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag...under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression of...about cloning & additional plasmids. Aebischer and Trono 11795 pLL3.7 3rd Expresses shRNA under mouse U6 ...

...targeted neuronal manipulation. Topics may include: How this technology is used for targeted neuronal manipulation...Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September 21, 2018 Contact... AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional control). The...fit in the big picture of methods for targeted neuronal manipulation? Best practices for using this techonology... is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables, data, or images...communication representing a reference for AAV use in neuronal intersectional control. Through your participation...Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Haery L, Deverman BE, Matho KS, ...

...pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII...newborn neurons Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral...CREM (or Cre-M) has been engineered to contain an intron, preventing Cre expression when cloning in E. coli...including the polyadenylation site and several introns tet-hCMV Mammalian Sauer 12168 pMB80 (R26-CreER...AAV Aebischer 24704 GFAP-Cre Cre GFAP Mammalian Sofroniew 25997 LV-Cre pLKO.1 Cre and shRNA coexpression...pmSyn1-EBFP-Cre Cre-EBFP fusion; Expression in neurons. synapsin AAV Zeng 51904 paavCAG-iCre iCre CAG ...AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA...

...Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that contains an intron in the middle ...expression constructs available that are driven by a strong constitutive promoter and can be used to monitor...so both Renilla and Firefly luciferase contain introns Anthony Leung 141285 pGWB401NL3F10H NanoLuc® Creation...Top Expression Constructs Luciferase driven by a strong constitutive promoter and can be used to monitor...firefly luciferase Scott Lowe 14080 pAKlux2 Lux Operon Bacterial expression of luciferase from broad host...expression of firefly luciferase and iRFP720 from a bicistronic promoter Antonius Plagge 118017 pLX313-Firefly...