We narrowed to 14 results for: RON

...expensive to ensure adequate quality, but this upfront cost will save headaches and expense later. At ...that are suitable for unstable or recombination-prone DNA. The use of electrocompetent cells is essential...commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library DNA (100 ng...

...A or T between position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats...must follow safety procedures and work in an environment (e.g. BL2+) suitable for handling HIV-derivative...responds differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin...

... do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the...position. Sometimes an “N” is the result of an erroneous insertion by the sequencing reaction....

... to be centrifuged Water Background Information Front view of an open tabletop centrifuge with labels ... same components. The photo to the left shows a front view of an open tabletop microcentrifuge with the...

...chemicals that present a danger to people and the environment in biohazard containers. Biohazardous materials...protocols require bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2...

... 2 : Provides an optimal and stable chemical environment for the DNA polymerase to work adequately. Divalent...solvent and buffer capabilities provide a suitable environment for the DNA amplification reaction. Reference...

... strain grows, it's possible that: You used the wrong antibiotic. Your antibiotic is at too high a concentration... strain grows, it’s likely that you’ve used the wrong antibiotic, or confused your strains....

...any growth after overnight incubation. What went wrong? Try growing the culture for more time. Some bacterial...

...and 30 bp annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins...

...instructions for each. I got no transformants. What went wrong? Check that you are plating on an LB Agar plate ...

... cheap and accessible) to massive transmission electron microscopes that allow us to see cellular components...

...Background Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger ...

...product is the anticipated size and that your band is strong (indicating that the PCR reaction worked and that...

...If the samples are running unevenly and the dye front looks like a “smile”, reduce the voltage. Turn off...