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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 14 results for: RON

Showing: 1 - 14 of 14 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...expensive to ensure adequate quality, but this upfront cost will save headaches and expense later. At ...that are suitable for unstable or recombination-prone DNA. The use of electrocompetent cells is essential...commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library DNA (100 ng...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...A or T between position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats...must follow safety procedures and work in an environment (e.g. BL2+) suitable for handling HIV-derivative...responds differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin...

  3. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the...position. Sometimes an “N” is the result of an erroneous insertion by the sequencing reaction....

  4. Centrifugation

    Type
    Protocol
    ... to be centrifuged Water Background Information Front view of an open tabletop centrifuge with labels ... same components. The photo to the left shows a front view of an open tabletop microcentrifuge with the...

  5. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...chemicals that present a danger to people and the environment in biohazard containers. Biohazardous materials...protocols require bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2...

  6. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... 2 : Provides an optimal and stable chemical environment for the DNA polymerase to work adequately. Divalent...solvent and buffer capabilities provide a suitable environment for the DNA amplification reaction. Reference...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ... strain grows, it's possible that: You used the wrong antibiotic. Your antibiotic is at too high a concentration... strain grows, it’s likely that you’ve used the wrong antibiotic, or confused your strains....

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...and 30 bp annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins...

  12. Pipetting Protocol

    Type
    Protocol
    ...Background Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger ...
Showing: 1 - 14 of 14 results