We narrowed to 242 results for: gRNA

1. Twenty Years of Sharing: Expanding Accessibility

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   ...Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go...

2. Plasmid Collections

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   ...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...

3. Bikard Lab - CRISPR Repression Collection

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   ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard ...

4. CRISPR-mediated Plant Base Editors

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   ...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...

5. Fluorescent Proteins 101: When GFP lets you down

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   ...can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein, a fusion with GFP allows...

6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity...Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised ...

7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ...linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave the 22kb vector. When followed...

8. Neurodegeneration Plasmid Collection

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   ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76809 PANK2 gRNA (BRDN0001149459) PANK2...76810 PANK2 gRNA (BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362... PANK2 gRNA (BRDN0001144887) PANK2 hU6 Hallervorden-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606...PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76840 PRKAR1B gRNA (BRDN0001147089...

9. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic Locus of Interest Browse ...

10. Hot Plasmids: Spring 2025

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    ...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...

11. Sequencing Options for CRISPR Genotyping

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    ...position of the mismatch along the length of the gRNA given the stricter sequence requirement at the terminal...many software tools predict off-target effects of sgRNAs using computational methods. They identify possible...mismatches based on the sequences of the genome and sgRNA. This is a good starting point for most researchers...

12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... Gateway compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors...Plasmids and Resources collection, and browse our Empty gRNA Expression Vectors page Cre-lox Site-specific recombination...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...- Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral...Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops...Lentiviral expression plasmid for two sgRNAs from an hU6 and an mU6 promoter Protein-protein interaction...confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice...

13. Microbiology Resources

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    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

14. Worm Expression Resources

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    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...

15. Allen Institute for Cell Science Plasmid Collection

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    ...including: data and images from the hiPS cell lines gRNA sequences used in the creation of the hiPS cell ...

16. Zhang Lab CRISPR Page

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    ...CRISPR Cas9 gRNA plasmids from the Zhang lab...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse...CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven... SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran... pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241:...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus ...cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming, have been typically optimised... the in vitro-transcribed single guide RNA (IVT sgRNA) approach (Dewari et al. 2018 and Figure 2). Not...construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure 2: Cas9 RNP complexed with 2...

18. Plasmids 101: Modular Cloning Applications and Kits

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    ...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...assemble multiple unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This ...for the multiplexing of sgRNAs against unique genomic targets or multiple sgRNAs against the same target...target to ensure efficient cutting. Beyond sgRNAs, MoClo can also be used to test a variety of Cas enzymes...

19. Plan Your Experiment

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    Guide

    ...how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected...enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence ...delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then... Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are combined...modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and target...the associated gRNAs based on their predicted on-target and off-target activity ( see gRNA design software...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

20. Zebrafish Plasmid Collection

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    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...