...human kinases remains challenging. Small molecule kinase inhibitors usually target soluble kinase domains...Human kinase domain constructs for automated bacterial expression The misregulation of human kinases has...recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed...Threonine kinases while YopH (residues 164-468, Plasmid 79749) enhances expression of Tyrosine kinases. All...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion would...Arumugaswami lab at Cedars-Sinai Medical Center has kindly constructed and deposited an unpublished set of...

...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...plasmid maps!" After thoughtful design, vetting, and tweaking, we’re excited to announce that our plasmid and...GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive...identities of most features labeled directly on them making them much easier to quickly identify. Improved ...With this more functional display, you can start thinking about your next cloning experiment early on. Beyond...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...similar hover over features. As with the map tab, clicking on two separate locations in the linear sequence...

... and then condensing that and making it accessible to students.” Making science approachable is often ...This involves going in front of a camera and kind of walking them through the steps of, for example, cutting...And I’m like, 'Well, I’m here so let’s do it!'" Working as a subject matter expert Caitlin started out ...job as an SME: “I write curriculum for students taking science courses online, which is a blast. I have...an introduction that gives a basic overview of kinematics in physics, to developing a lab about mitosis...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...courses encompass what they want to teach.” Besides working with students and faculty, Caitlin also collaborates...

...basic one, making sure that you have a good fundamental understanding of what you’re working on is important...years. We each have a unique “origin story,” but taking the first step into the world of academic research...glassware with brightly colored hazardous liquids. Working in a lab is nothing like a typical part-time or... and applied. The current pandemic serves as a striking example of the applicability and importance of...be unsure, it helps to have a vague sense of what kind of research you would be excited about. I know quite...do what they do. I would also highly recommend talking to other undergraduate researchers, as well as ... yourself into is really important.  As you’re talking to people, though, try to be conscious of who has...

...thawed and used to produce a set of ‘working’ stocks of cells. Working stocks should be frozen in multiple... generally ensure cell line identity and purity making it easier for comparable studies to be performed...to check their identity. For example, perhaps a working cell bank has been made by a research institution... good recordkeeping, you need to have a way of tracking how many passages the cells have undergone and... any of the Master cell vials until all of the working vials have been used up. This ensures you can retain...means you can regularly discard the cells you are working with because you have plenty of maintained freezer...freezer stocks. It is vital that the Master and Working stocks of cell lines have the passage number recorded...

...available for tracking certain cell types. Another useful tool for marking and tracking cells is the the...tools allow one to study the kinetics of cell migration and movement by marking cells at a specific time.... Pathogens - Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization...tissues, including lymph nodes, brain, liver, gut and skin. Two-photon intravital microscopy. The video above...that some are more reliable and more efficient at tracking cells. Good two-photon probes when using a standard... cells and follicular dendritic cells within the skin and lymph node respectively. The permanent labelling...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     The past...

...release IL-36 cytokines. Keratinocytes are the main source of IL-36 cytokines in the skin making it important...the uncontrolled activation of the interleukin-36 (IL-36) cytokine family resulting in the activation ...disease research. This year is our fourth year working with the Rare Genomics Institute by providing plasmid-based...pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000...surrounding IL-36 biology. “Little is known how IL-36 cytokines actually get out of keratinocytes and induce their...researcher at University Hospital Zurich. IL-36 cytokines are a potent inducer of an autoinflammatory loop... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

...qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind of plate they...professionals from other backgrounds at conferences or networking events. And if you’re a wet lab scientist who...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...they were talking about during the meetings. And “plate” certainly wasn’t the only problematic word. How...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...creating shared experimental protocols. Clarify: Asking for clarification during a meeting can help get...

... worth taking time to honestly evaluate your own style of work and communication before making this important...attention you need? Are you good at asking for help? If you are good at asking for and getting help, size might... toss data-driven reasoning out the window when making decisions. We think scientists make choices based...based on emotions and assumptions. I’m not just talking about simple things like “Which route should I ...members can give you a fuller picture. Consider talking to people in the lab across the hall or in the ...prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...t choose a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting...

...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...type of team can exist indefinitely. If you’re looking for this type of team in academia, look no further...an imaging core or tissue culture core, you are looking at a process-oriented team serving a particular...you find that there is not someone (or someones) taking on these responsibilities on a team to which you...is certainly necessary, but equally important is making sure all team members understand what roles they... none of that matters. If you are on a team, any kind of team, and you have nagging doubts that someone...

...of my job], so making it a success feels like a real accomplishment.” He started working on them shortly...not sure what his next step should be. He debated taking a position as an application support/sales representative... to me. She was leaving a writing position and looking for someone who might take over. I like writing... a science writer these days. I do some public speaking and a bit of video work for webinars (get tips...shortly after joining IDT when his boss was looking for someone to take over hosting them. Since then, webinars... writing group I am part of is responsible for checking the scientific accuracy of everything that goes...analysis.” Han’s biggest piece of advice for those looking to break into the field of science communications...

...recognize their MANY transferable skills learned while working in the lab and offering advice on areas where you...using creative problem-solving in the lab   Thinking Outside the Box: Individual Approaches Whether...something tangible and applicable to your situation.   Thinking Outside the Box: Team Approaches At some point...same problem. If your lab/company/team is still working towards a culture of encouraging dissent, the next...nine enemies that stand in the way of creative thinking. I won’t go through all nine here, but I’ll discuss...longest tenure, but don’t let that stop you from speaking up.  If your ideas are not well received, understand...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

...back out when you are looking to change positions in the future. Anyone looking for work in today’s job...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...student or postdoc forever, and you need to be thinking about what comes next. To that end, one of the...for your career is to expose yourself to other working professionals, and one way to do this is to attend...and connections are how you get jobs! Job fairs Speaking of jobs, it seems like an obviously important ...conference, job fairs bring you face-to-face with working professionals, with the added benefit that most...larger meetings offering resources for students seeking employment within industry or academia; although...

...cells. These cells produce inflammatory cytokines - interleukin (IL)-12 and interferon gamma (IFNγ) - and...help escape immuno-surveillance, and curb cytokine and chemokine production in the tumor microenvironment...their microenvironment by producing C-C motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive immune...tolerance beyond direct proximity to the tumor; chemokine-aided activation of APCs produces modified dendritic...cancer. Surgical removal causes increases in tumor cytokines like TGFβ and IL-6. However, when strengthened...pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research include...response. Antibodies targeting the tumor-augmenting cytokines produced in the tumor microenvironment TGFβ, IL...

...discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for...lead to hurt feelings, low morale, poor decision making, and stalled projects. You want to identify and...experiments to address a reviewer’s concerns, you are making your hypothesis stronger. When you share data at...miscommunications in the future. Assumptions: Making incorrect assumptions can be a direct cause or ...or consequence of lapsed communication. Making some assumptions about how something should be communicated...know that a person or team should be involved in making a decision and you see no record of their input... an eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion...

...addition, it’s really asking a lot of someone to be comfortable on camera while thinking about things like...Science Video Production Specialist, I’m constantly thinking about ways to improve our productions to deliver...we developed a great plan to start consistently knocking out high-quality videos for you all. The next ...products or methods, using metrics and feedback, taking a look at what works and what doesn’t, and trying...Design-Build-Test-Learn cycle adapted for video making.   Cycle One: The Ironing Board Control Center...Center With the pandemic in full swing and everyone working from home, how were we going to film anything? ... prove to be difficult. Shipping the gear, and walking everyone through the recording process was going...

...wise to start working on these skills. Neuroscientists use lots of cool tools While walking around the exhibition...discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster presentation...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that almost... mathematical know-how for analysis. If you’re looking to get into neuroscience and you don’t have a background...neuroscience but are afraid you don’t have the right kind of biological expertise to get into the field, there...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers... suggest a plasmid to us, request that we start making the AAV, or help us produce new educational content...

...collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making available.... Addgene: Is this what you are working on now? Voigt: We are working on this now, we have ideas, but ...Massachusetts Institute of Technology has been “working on new experimental and theoretical methods to ...large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits become... been putting together very large toolboxes and making them available. We’ll also be submitting parts ...objective is to give people the complete toolbox for taking on very large genetic engineering projects. Addgene...you want. The last few years, we’ve really been making tons of parts and characterizing the software to...

...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...with the end of year approaching. We’re always looking for something to brighten our day, whether it’s... Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission...microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity, and photostability...pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs) are a major class of signaling receptors...spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. bioRxiv 2022.08.13.503850... al. 2022). iGECInano displays better response kinetics and photochemical characteristics than previous...

...and foreign nationals working in the life sciences community obtain their working visas, permanent resident...national researchers and entrepreneurs who are looking to continue their careers in the United States....we get elected to Congress, we are tasked with working with US immigration in its current state. As of...discussed in this post benefit from the applicant thinking about the process early, keeping good records,...trained scientist. If the candidate is ultimately looking for a role in a different functional area, then...received sponsorship) then he or she can start working in October of the same year. The Industry H-1B ...of US government grants that the researcher is working under in the lab or has won, awards, media reports...