...LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted Dawson 17613...William Hahn 23955 YFP-Parkin PRKN YFP CMV Parkinson's Richard Youle 23956 mCherry-Parkin PRKN mCherry CMV ...2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2-LRRs LRRK2 Myc CMV Parkinson's Mark...His polH Parkinson's Michael J Fox Foundation MJFF 40385 pBACgus4x/Parkin PRKN polH Parkinson's Michael...PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's Mark Cookson...PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson...PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-V5 KD PINK1 V5 CMV Parkinson's Mark Cookson...

...repeat cycles of expansion. Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...other cytokines, enable communication between cells of the immune system. Interleukins Interleukins are ...factor FLJ40431 Return to top Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...CCL14-CCL15 chemokine ligand 14, chemokine ligand 15 transcription unit - CCL15 chemokine (C-C motif) ...CCL27 chemokine (C-C motif) ligand 27 ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27 CCL28 chemokine (C-C...CX3CL1 chemokine (C-X3-C motif) ligand 1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin...SCYB14, bolekine CXCL16 chemokine (C-X-C motif) ligand 16 CXCLG16, SR-PSOX, SRPSOX CXCL17 chemokine (C-X-...

...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated...STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2... CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2 Connector enhancer of kinase suppressor of Ras CYTH2...receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2...coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1 RPS6KA2... Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1 RPTOR Regulatory associated...homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho GTPase activating protein 35 ARHGEF2...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...motor neurons leads to limb weakness, difficulty speaking, swallowing and breathing, and often results in...movements, memory deficits, as well as difficulty speaking and swallowing. HD is caused by abnormal expansion...Symptoms include memory loss, deficits in decision-making and language abilities. The disease can be classified...mammalian axons using lentiviral vectors expressing kinesin heavy chain 5 . Zamponi et al. Dev Cell. 2022 Jun...GRABDA or the calcium indicator GCaMP6s allows tracking of dopaminergic signaling and neuronal activity...

...growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1 and mTORC2...homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin...S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von ...LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as ...as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin ...; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB... PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC...

...pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI...heteroduplex resolution. The process of double nicking, however, increases indel formation slightly. Designing...the edited strand, as in the PE3b system, guides nicking of the unedited strand only after the edit has ...promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and ... No mRFP1 David Liu QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao pYPQ141D-peg Plant...Tilmann Weber pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber pPBT-peRNA_GG-Puro Mammalian...sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim pDAS12069_U6-pegRNA-mCherry...

...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...

...Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 ...Knockout Human Doench and Root 3rd 4 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout...(Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang... Human Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 ...Library 140633 Knockout Human Wei 3rd 3 58,630 Human Kinase Domain-Focused CRISPR Knockout Library 117725 Knockout...

...Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details the use of CRISPR...Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp. (95), e52118, doi:10.3791/52118...deletion). Use a pair of forward and reverse primers flanking each sgRNA target site (within 150 - 350 bp) to...Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos...This is a representative sequencing primer; other flanking primers may be utilized. Choose a sequence-verified...at 400 x g for 5 min and remove supernatant by flicking the PCR plate over a sink. Add 50 μl of DNA extraction...

... ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV-...infection induces a cytokine storm response in the body. For a full list of cytokines and related plasmids...family called SARS-CoV-2. Scientists have been working at lightning speed to elucidate as much as they...and Yeast expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute...

...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

...Single-Strand Break (Nick) , Double-Strand Break (Cut) Making Precise Modifications Using Homology Directed Repair...a specific sequence adjacent to the RNA target, making this a very flexible editing system. Editors based...sites was sufficient to repress transcription by blocking initiation. dCas9 can also be fused with transcriptional...target genes. In contrast, CRISPR is capable of making highly specific, permanent genetic modifications...Multicolor CRISPR imaging allows for simultaneous tracking of multiple genomic loci in living cells. One ...fluorescent CRISPR system has been used for dynamic tracking of repetitive and non-repetitive genomic loci,...Another limitation of SpCas9 is the low efficiency of making specific genetic edits via HDR. For specific point...

... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...111705-8 ) is available from Addgene. Screening kinases. The mode #1 phosphosite library can also be synthesized... Nat Biotechnol. 2018 Aug;36(7):638-644. PubMed Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented...Communications . 2015. 6, 8168. PubMed The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a Program...

...are some safety considerations to evaluate when working with lentivirus: these viruses are based on HIV...components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient lentivirus Rawlings , Scharenberg...2nd and 3rd generation lentivirus technologies. Looking for lentiviral plasmids to express your CRISPR ... ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 ...inducible knockdown, multicistronic design enables tracking of viral transduction. See article for more similar...

...relevant to Parkinson's disease created by the Michael J. Fox Foundation... Plasmid Collections Parkinson's Disease...MJFF Plasmids Shared Research Tools Addgene is working with The Michael J. Fox Foundation (MJFF) along...along with individual laboratories to assemble a Parkinson's disease plasmid resource for the scientific research...

...brighter fluorescence and faster kinetics (CaMPARI2) Improved methods for marking active neuron populations....fluorescent calcium sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically... novel analytical method for in vivo phosphate tracking. FEBS Lett. 2006 Oct 30. 580(25):5885-93. Wolf... Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission...Glutamate iGluSnFR glutamate sensor with improved trafficking to the plasma membrane Acute disruption of the...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.... Vladislav Verkhusha Return to Top Browse All Looking for a sensor for a different analyte, a sensor ...

...Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice ...applications. Full references are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent... for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224 : sgRNAs...10.1038/nbt.2647. Epub 2013 Jul 21. PubMed . Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing...plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Platt...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

... to have 50-80bp of homology arms on each side flanking the change you'd like to introduce. We use Ultramer...plasmid donor, with two homology arms on each side flanking your desired insertion or mutation. Each arms ...choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate the PAM 'NGG...plasmid donor, with two homology arms on each side flanking your desired insertion or mutation. Each arm is...

...important factor (for instance, looking for a different conjugate or looking for antibodies validated for...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ... assay, called native ChIP, does not use cross-linking and instead relies on strong interactions between...the product information for similar antibodies, looking for ones that have identical features and epitopes...