...less phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs...fluorescent kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest...proxy for kinase activity.  Advantages of KTRs When compared to FRET reporters for measuring kinase activity...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...for regulating signal transduction and measuring kinase activity at the single-cell level can aid in drawing...phenotype. One method for monitoring live single-cell kinase activity is FRET, but FRET reporters are challenging...Covert Lab provides an alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization...

...am currently working on projects for the Science Communication function at Addgene. Working a 9-5 As it...one semester (the majority spend two to three) working in your chosen field. This cooperative education...bioengineering student, to Addgene. I am currently working on the QC/QA team as a DNA Sequencing Intern. ...interview was intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved...environment on my team and at Addgene, I felt comfortable asking questions without fearing they would be disregarded...addition to these core hours, we have flexible working hours. For Addgene that means that it is possible...

... such as nutritional status. Within the animal kingdom, this information is relayed through factors (both...such as genes involved in metabolism, growth or cytokine action). Primary or core coregulators (those that...cellular roles. In the current phase of NURSA we are seeking to compliment these consortium-generated datasets...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

...side conversations within the team. We are not talking about gossip here – this means encouraging a culture...Connie Cepko to learn about her management style.   Seeking feedback frequently One of the most important paths... only way.   From a colleague: MBWA "Manage By Walking Around" – don't hide in your office. Stop by instead...

...processing app—revolutionary and the first of its kind but lacking the ability to do anything other than cut/...

...stories, and other 20th anniversary celebrations, by checking out our blog, social media, newsletter, and website...typically adopted by for-profit companies, while making use of our nonprofit status to achieve mission-...scientific community. In 2024, we will be actively seeking ways to implement these values in everything we...the Addgene blog 2022 and 2023: Reflections and Looking Forward...

...relationships because networking has gotten a bad reputation (as in…”I just hate networking”). Scientists enjoy... having to talk with strangers. Be bold in asking all kinds of people to connect with you and to share...the nonacademic sphere where most of us will be working.  Check out Joanne's Reddit AMA It should be no...fine to ask about specific jobs (that is what networking is about), but be upfront about interest in a... yes and they might say no but do not confuse networking for a job connection with ongoing relationship...relationship building through Informational Interviews. Making Your informational interviews productive Informational...remote conversations. In this interview you will be asking the questions, so be prepared. There are many good...

...jobs where all I did was public speaking. If you're also nervous, try taking a few deep breaths. Then, identify...breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking as a scientist...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an...support for your ideas, invite others into decision making processes, and even interview better! As a scientist...science communicator, I’ve done a lot of public speaking, and I'm happy to share what I've learned along...When my mind went blank, my options were to stop talking until I could present something coherent or to ...me accomplish my goal.   As a scientist, public speaking isn't a performance art. You’re (usually) presenting...

...accomplishments from 2022 and share what we’re looking forward to in 2023 and beyond. Last year, we distributed...rapidly changing environment. In particular, we are working to understand what challenges remain in materials...changing landscape of science. We are therefore embarking on a strategic refresh to understand this new ...help a great number of people. I look forward to working with Addgene colleagues, collaborators, partners...

...Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...subset of kinases. The Problem:  There is no common method for creating optically controlled kinases. The ...design for creating photoswitchable kinases and use MEK1 as a model kinase. In this system, light is used ...photoswitchable kinases: psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar ...photoswitchable kinases good for? There are a number of potential applications for photoswitchable kinases.  Below...Zhou et al. 1. Testing kinase inhibitors Zhou et al examined the ability of kinase inhibitors to alter Raf1... by intermediate messengers. Optical control of kinase activity can provide greater spatiotemporal resolution...

...as coming from networking at the time, but that means it was the best kind of networking! Since nearly ... parents looking for experts to come give guest presentations. If you’re a scientist thinking about reaching...was very much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was...my community. This past year, I spent some time thinking about how I could combine all of these roles by...space, time events, and network for volunteers. Speaking of volunteers, you can’t run an event like this...call to local parents who would feel comfortable speaking on topics that would compliment the STEM curriculum...been aimed at grad students, postdocs, and other working scientists, creating a presentation for 8th graders...

...gene expression of cells at a single time point, making it difficult to investigate how early expression...and cell identity at the single-cell level, thus linking ancestral gene expression patterns with reprogramming...reprogramming outcomes (Biddy et al., 2018). Tracking lineage and cell identity at the single-cell level with...re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path. The CellTags revealed that...intestine, demonstrating its utility in long-term tracking in vivo (Guo et al., 2019). Key takeaways of CellTag...cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics of gene expression and clonal...cells or the clonal evolution of cancer. If you’re looking to use CellTag, you can find the CellTag libraries...

...of cells. Looking ahead What’s next, you might wonder? Verkhusha says they are always looking to improve... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ... and intensity allows for optical labeling and tracking of proteins, organelles, and living cells in a...improve upon existing genetically-encoded probes by making them brighter, more photostable, smaller, and less... the 750 to 800 nm range, and his team is also looking in the direction of biosensors capable of revealing... muscles work inside a living animal. For those making use of these probes, he says, when in doubt, read...

...about replying and thinking about good questions and answers. Professional networking groups will often...Check out Joanne's Reddit AMA Who should I be looking for? Be on the lookout for friends or colleagues...who gives a great talk, ask them for advice on speaking. If you see someone with a great professional ...t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships for Success”. Choose...Choose 1-2 tactics to try. One of my favorites is making the pledge to have lunch or coffee with someone...started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes a good...to organize a successful peer mentoring circle. Asking someone to be your mentor Mentoring is a “loaded...

...definitely recommend checking out GCE4All’s website and resources, and then checking out all of the plasmids...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...reagents!) for the use of GCE. In short, they’re working to make GCE available for all biomedical researchers...

...founded in 1944 with the goal of facilitating networking between chemistry students, especially those ...scientific disciplines, as well as social and networking events. Imperial Synthetic Biology Society, ... synthetic biology by providing education and networking opportunities for society members as well as ...in society and to support iGEM alumni-industry networking to promote the creation of start ups and biotech...students and the general public, career events, and networking meetups. The society hopes to help young scientists...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn... networking...

...Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you... events will generate these types of mutations, making it an ‘easy’ editing job to get done. However, ...observed when inserts are within 10 bps of the break, making the cut off essentially as part of the templated...

...Streaking for single colonies is an integral part of any bacteriologist’s skill set. So when Dave Westenberg... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave... suspension,” says Westenberg. In addition to streaking for single colonies, these chromoprotein-expressing...

...when you can, instead of just asking for favors.  Don’t focus on networking with “important” or senior people...helpful who you’d like to get to know better. Networking is not about how many contacts you have; it’s...become a volunteer). Naturally running into or working with the same people is often the easiest way to...get a response. It breaks an important law of networking--don’t be creepy. There is a person on LinkedIn...they don’t know me. It’s a waste of our time.  Networking connections can last years This is a graphic ... the Addgene blog Browse other blog posts on networking Attending a conference soon? Get tips here Learn...

...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more...contributed by guest blogger, Dalila Cunha de Oliveira. Bricking Science is an idea built, literally, 'brick-by-brick...Running Smoothly and "Gnome-Proof" This is why the “Bricking Science” YouTube channel was born. The name came...with each other through globalized, collaborative thinking. LEGO and science: The potential is endless One...feet), to connect people with science. And so, “Bricking Science” was born. We chose the stop motion format...approachable for LEGOs. Stop motion consists of taking many pictures, one for each position in a movement...video takes an entire week of our free time. After taking almost a thousand pictures, my husband Enzo will...