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We narrowed to 532 results for: lias

Showing: 181 - 200 of 532 results
  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-transduction... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...
  2. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...culture and who was extremely helpful since she was familiar with library generation. Also, I now know what... next workshop, I’ll ask myself: What are the familiar parts of this workshop? What do I understand ...
  3. Science Career Options

    Type
    Blog Post
    ...requires travel can show that you are self organised, reliable, and can work independently. Practical outdoor...are highly desired, as roles are becoming more reliant on technology, and good computer skills will give...
  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag...CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag...Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag...MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid ACP...
  5. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime... they emit or to optimize their expression in mammalian systems. Our luciferase collection page has a ...
  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...envelope, Phoenix-AMPHO (for the infection of mammalian cells.) Using this system, virus is produced in...of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc...
  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing...RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021)....
  8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, Zhao T-T, Connelly JP, Pruett-Miller...
  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... Drouin, L. M., Jia, N., Deng, J., Hebben, M., & Liao, J. (2024). A Comprehensive Study of the Effects....2019.276. PMID: 31752513. Zhou, Q., Tian, W., Liu, C., Lian, Z., Dong, X., & Wu, X. (2017). Deletion of the ...
  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...This post, along with a future companion post on mammalian vectors, will catch you up on the core replication...and ability to be used in bacterial, yeast, and mammalian cells.   ...
  11. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...Click Biology describes the search for robust, reliable reactions, using only nature’s building blocks...Click biology highlights the opportunities from reliable biological reactions. Nature Chemical Biology....
  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...microbiome species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...
  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on ...RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely due to the lack of collateral...
  14. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...an international research team from Norway and Australia worked with stem cells in mice. They appropriately... of cells and tissues—when that information is reliable—is important to ensuring excellence in basic research...
Showing: 181 - 200 of 532 results