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Showing: 181 - 200 of 861 results

  1. Summer SciComm: Preprints

    Type
    Blog Post
    ... and comment online! Want the general public (say, policymakers or community organizations) to use your...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, Twitter...scientific manuscripts shared by the authors on a public server independent from peer-reviewed publishing...accelerating science! Photo courtesy of under a CC license. Many scientists and advocates have argued ...increasingly growing time from submission to publication. It takes many months, often more than a year...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...and updated by the authors over time, providing a public record of changes, critiques or endorsements. In...

  2. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...Organizing a science conference for the public How does one organize a public scientific conference? As a member...DayCon is a one-day conference aimed at the general public that provides an accessible introduction to various...the communication gap between scientists and the public and help make science more transparent. Second,... their communication skills and engage with the public. The organization runs a wide variety of programs...conference-style experience that was accessible to the general public both in terms of cost and content level. Sponsorship...science outreach can be, both for scientists and the public. For students, these opportunities to improve our... alternative careers in science. In return, the public receives trustworthy, intelligible scientific resources...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M...doi.org/10.1038/nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, ...10.1038/ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu.../j.cell.2016.08.056 Find plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...searches, such as clicks to read the abstract or full text of the paper, publication date, a relevance...show all publications by an author, total number of citations, and the number of publications that heavily...that term. You can also set up alerts for new publications from a particular author. Saving papers Papers... work, so it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 ...organizations, and universities often tweet about publications in the fields of research they support. Monitoring...Artificial Intelligence. Semantic Scholar analyzes publications and figures using machine learning to extract...your search to a specific window of time for publication. To help you quickly identify papers with greater...

  5. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...the OpenWorm website. microPublication microPublication is a unique publication for the worm community...into larger publications and would otherwise be lost in lab notebooks forever. microPublications are peer-reviewed...aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of mRNA are important ...normally one figure and can be negative and/or even replicated studies to show reproducible results. This a ...referenced. In addition the data published in microPublications is curated and added to other databases including...including wormbase and flybase. microPublication is run and maintained by members of the worm community...introducing point mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These are...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...mouse IgG2a and rat IgG1 antibodies in the same application, using a Goat anti-mouse IgG2a cross-adsorbed...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... tools for familiar applications. But how does one invent a tool for applications that don’t even exist... Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that ...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible. But ...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that...reproduced from Frank Hayward et al. 2024 under CC-BY license. They did more experiments to see if any small...reproduced from Frank Hayward et al. 2024 under CC-BY license.   Video 1: Fluorescence movie of E. coli ...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...intended application - Remember, just because your antibody performs as expected in one application does ...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...intentionally generalizable and not specific to certain applications or antibody targets. That said, not every strategy...will be suitable for every antibody or every application. The goal is to show that your antibody has been...strategy to use will depend on your intended application and available resources.     Figure 1...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, ...alternative translation start sites or alternative splicing that could lead to some form of the target protein...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications Organic...labeled through click chemistry. Although these multi-component systems can be complicated to implement,...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...adapted from (Bottanelli et al., 2016) under CC-BY license. Combining orthogonal self-labeling tags on...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...reused from (Ulrichs et al., 2023) under CC-BY license.   But these tags aren’t limited to fluorescent...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    ...suppliers, and public health and medical organizations are working to help educate the public about proper...containing a phage origin of replication that can be packaged into replication-incompetent phage particles...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving...essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target such...mammalian viral vectors in that the vectors are replication-deficient. Thus, a very large number of phagemids...CRISPR antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages...

  11. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...for both transient and stable gene expression applications in plants. This smaller, easily customizable...Agrobacterium are termed binary vectors because they must replicate in two species: Agrobacterium and E. coli. Like.... coli. However, they also need to be able to replicate in Agrobacterium to ensure that they are maintained... Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium, along...bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they...constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized in studies...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...

  12. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...list of individual screens curated for the publication. Clicking on the screen name takes users to a display...favorite gene was described in a CRISPR screen publication. You see this mentioned in the results section...manually curated and annotated from more than 45 publications, beginning with the seminal research articles... makes it possible for researchers to query all public CRISPR results for any gene of interest. In addition...screen curated in ORCS is linked to the original publication via its PubMed identifier, which can also be ...screens of interest.   Figure 3: Each publication featured in BioGRID ORCS has a dedicated summary... supplemental data tables available with the publication are also provided for the user. This approach...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    ...for your application. Image reused form Doman and Pandey et al., 2023 under CC-BY 4.0 License. Find... CreVLPs, and eVLPs for in vivo and in vitro applications.   Figure 2: Modular features of DIRECTED...Strebinger et al., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can...approach for experimental therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D...interactions or map a local proteome, but some applications have been challenging or inaccessible. The Ting...alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable...from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0 license. This system provides a unique new way to record...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...to disparate physiological effects and complicate any metabolic study. The Ralser lab has created a unique.... Seeliger MA, Young M, Henderso MN, Pellicena P, King DS, Falick AM, and Kuriyan J. Protein Sci. 2005...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...Wu Lab library can be found in the associated publication. Ma, et al. Cell Rep. 2015. PubMed PMID: 26190106...and ubiquitous reporter assay. In their recent publication, Kroeze et al. describe their enhanced TANGO .... Each gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...

  15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...emission wavelengths, which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These... substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby...typically bind to. Stability of fluorescence: For applications that involve ranges of pH, and significant lengths...light, is another factor to consider. If your application requires longer periods of exposure, you’ll want...have shown that different fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to ...descriptions, or technical manuals. Specific applications and matching dye/ligand choices For effective...beyond the diffraction limit of light. Dyes like silicon-rhodamine or Janelia Fluor (JF) dyes have specific...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and... In a column-based approach, the substrate is a silica matrix fixed in a column and you flow lysate and...settings, but when it comes to low-throughput applications, the choice is largely a matter of preference...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...Depending on the research question and downstream application, one or several of those techniques can be used...your flow analysis. However, certain downstream applications, like an in vivo adoptive transfer of those ...available fix/perm kits allow for labeling of cytosolic proteins, as well as proteins of the secretory...intranuclear proteins of interest, you can label cytosolic proteins alongside the intranuclear ones. You ...You do not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular...or free amines of proteins. Depending on the application, they can be membrane-permeant or membrane-impermeant...are positive or negative for the dye. The main applications of dye stains in flow cytometry are cell proliferation...

  18. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication. ITRs are...binding site for Rep78 and Rep68 to initiate replication and a terminal resolution site that serves as... Rep proteins are important for viral genome replication, these mutations can affect transduction efficiency... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and... and hairpin structures, ITRs are prone to replication errors and disruptive mutations and truncations...mutations?  During propagation, mutants rapidly replicate in a growth culture and result in a high percentage...

  19. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...what we do not understand, and the complicated and unique metabolic processes of plants are being investigated...involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the biggest...difficulties that plant biotechnologists face is public perception of genetic modification (GM) and synthetic...benefits and future potential of the technology to the public in an open, responsible manner. At Revolution Bio...obstacles to plant synthetic biology In addition to public resistance, an overall limited understanding of...knockouts to better understand developmental, metabolic, and photosynthetic processes. Often the tools... intellectual property concerns – patents and licensing agreements. Developing a product without the use...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic Acid Linkage...receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor2   AAV2 Heparan sulfate...Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor2 Platelet-derived growth...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...
Showing: 181 - 200 of 861 results