We narrowed to 210 results for: pcr

1. Addgene is Expanding Our Viral Vector Service!

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   Blog Post

   ...assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing, and viral genomic...

2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   ...advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan...

3. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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   ...for downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed ...

4. New and Upcoming Viral Vectors - December 2019

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   ...for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution...

5. CRISPRainbow and Genome Visualization

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   ... gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector...

6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ... in gene expression readouts from assays such as qPCR or microarray. But, aside from facilitating the ...

7. Viral Vectors 101: Voltage Indicators

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   ...Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked to a chromophore and functions as a photoreceptor...

8. Great Results Start with Great Standard Curves

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   ...viral vector production facilities frequently use qPCR with a standard curve to titer their viruses; food...

9. Hot Plasmids - August 2020

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   ... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

10. Finding nucleic acids with SHERLOCK and DETECTR

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    ...laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of SARS-CoV...

11. Viral Vectors 101: Preparing Pooled Libraries

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    ...cells.  While Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend...

12. Bacterial Expression Systems

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    ...PelB tags for localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized...ratio) Escherichia coli Christopher Voigt 65008 pCRISPReporter-mCherry Promoter activity Fluorescence (mCherry...

13. Neurodegeneration Plasmid Collection

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    ...Parkinson's Mark Cookson 29354 pCR8-DJ1-WT PARK7 Parkinson's Mark Cookson 29355 pCR8-DJ1-D149A PARK7 Parkinson's...Parkinson's Mark Cookson 29356 pCR8-DJ1-E64D PARK7 Parkinson's Mark Cookson 29357 pCR8-DJ1-M26I PARK7 Parkinson's...-LFA EIF2AK2 GAL VWM disease Harmit Malik 20123 PCRII-zSynC SNCB Lewy body dementia Aaron Gitler 20648...SOD1 GFP CMV ALS Elizabeth Fisher 26573 CaV2.1 (pCR3.1) CACNA1A CMV Spinocerebellar ataxia 8 Diane Lipscombe...

14. Quick Guide to All Things Lentivirus

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    Blog Post

    ...and the reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have...

15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post

    ...key, especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence...

16. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...used either with plasma membrane proteins (such as GPCRs, ion channels, or cell surface receptors) or with...

17. Antibody Validation for Flow Cytometry

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    ...RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and potentially the need to study protein expression...

18. Fluorescent Protein Guide: Biosensors

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    ...eabq8173. Yulong Li Norepinephrine nLight red and green GPCR-based NE indicators Sensitive multicolor indicators...Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based NE sensors A Genetically Encoded...

19. CRISPR Guide

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    ... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

20. Zinc Finger Consortium: Zinc Finger Arrays

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    ...cCGCTTCAGCggtctGTGGACTCGa zebrafish similar to grp151 (or gpcr-2037) OZ535 and OZ536 OPEN OPEN aGCCATCATCaggttcaGGATGAGCCc...