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We narrowed to 208 results for: pme i

Showing: 181 - 200 of 208 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about...can imagine not old-fashioned at all. With the development of new fluorescent pairs and the emergence of...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...deletions, insertions, and even precise point mutations. I think it will always have a place in every bacterial...experiment? Find the pTOX plasmids here! Continued development of new allelic exchange vectors Allelic exchange...clear advantages, and are hopeful that continued development of allelic exchange vectors will produce an array...

  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...disulfide bonds, made by molecular evolution 1 1Edited by I. A. Wilson. Journal of Molecular Biology 275:245–253...Principles and Clinical Application. Clinical and Developmental Immunology 2012:1–15 . https://doi.org/10.1155...reagent sharing platform for antibodies, the Developmental Studies Hybridoma Bank Learn more about the ...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    ... Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector...existing vectors, as well as research into the development of new adenoviral vectors. This post was written...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...and resources References Doran, K. S., Konieczny, I., & Helinski, D. R. (1998). Replication Origin of ...Tor, X. Y., Loh, P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...cells. The REPAIR (RNA Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ....  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants. These variants...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of...plants, including the development of crops with longer shelf life and the development of disease-resistant...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...shape and the absence of a rigid cell wall. Here, I will cover some of the best ways to tackle mycoplasma...cross-contamination. Make sure you keep all the required equipment and reagents inside the laminar airflow before...cryopreserved cells decreases with every subculture. Equipment Fumigate the laminar airflow and the lab facility...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I intron is a chimeric β-...splicing. If intron splicing does not occur in Luc-I, the produced luciferase is truncated and lacks enzymatic...pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11...reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014 Dec;11(12...transactivators available through Addgene. The development of dCas9 and dCas9-fusion proteins has dramatically...

  11. Optogenetics Guide

    Type
    Guide
    ...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and...Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, et al. 2011. Neocortical excitation... novel ChRs from other algal species and the development of synthetic variants to enhance the functionality...Channelrhodopsin Variants: Features, Limitations and Future Developments. Exp Physiol. 96(1): 19–25. PMID 20621963 Mattis...

  12. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...deaminase catalyzes the conversion of a target ‘A’ to ‘I’ (inosine), which is treated as ‘G’ by cellular polymerases...of donor DNA to facilitate repair. Since the development of CRISPR-mediated base editing (BE), these types...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition by...tumors and cancer. The immune response to the development of tumors is a complicated and dynamic phenomenon... Equilibrium - In a process analogous to the development of drug resistance in bacteria, the tumor cells... onset of cancer. Similar patterns of cancer development are observed in autoimmune disorders. Chronic...autoimmune disorders is also known to precede cancer development, for example, inflammatory bowel disease is associated...in the various types of cancer will fuel the development of immunotherapeutics. There are still many important...

  14. Lentiviral Vector Guide

    Type
    Guide
    ...Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996). In vivo gene delivery and... H., Yu, E. Y., An, D. S., Sabatini, D. M., Chen, I. S., Hahn, W. C., Sharp, P. A., Weinberg, R. A., &....2192803 PMID: 12649500 Timms, R. T., Tchasovnikarova, I. A., & Lehner, P. J. (2018). Differential viral accessibility...understand integration sites have led to the development of multiple genome-wide analysis pipelines to...barcoding libraries . Mouse Modeling Since the development of safer lentiviral vectors, they have been seen...differences. Molecular Therapy — Methods & Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm...sites. Molecular Therapy — Methods & Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...effective for T cells transduction for instance. I hope you will find this information useful for your...sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...was a disadvantage particularly for clinical development. In addition, MoMLV is inefficient at infecting...

  16. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...direction and scope of the subsequent two. Consequently, I will focus on different approaches and considerations...Although all these steps are areas of active development, the first step necessarily dictates the direction...: a mechanism underlying genetic robustness. Development 147:dev186452 . https://doi.org/10.1242/dev.186452...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...PMC516055. 23. Pascal, John M., et al. "Human DNA ligase I completely encircles and partially unwinds nicked ...conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase. Using this method... PMID: 23142428. 17. Hamada, Mariko, et al. "Development of a ligase detection reaction/CGE method using...PMID: 23463388. 18. Hommatsu, Manami, et al. "Development of a PCR/LDR/flow-through hybridization assay...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting...Hooper, PhD, has also worked with R&D for product development and now specializes in supporting Promega cellular...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...T. Dargitz, R. Wright, A. Khanna, F. H. Gage, and I. M. Verma, “Functional Gene Correction for Cystic ...efficient manner than ever before, thanks to the development of site-specific nucleases (CRISPR/Cas9, TALENs...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated Anterograde Transsynaptic Tagging...vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org/10.1038/mtm.2016.62...
Showing: 181 - 200 of 208 results