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We narrowed to 602 results for: she

Showing: 181 - 200 of 602 results

  1. CRISPR Plasmids - Plants

    Type
    Collection
    ...52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31 rice snoRNA U3 BsaI none S. pyogenes...

  2. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR blogs CRISPR tips, FAQs, and news published on the Addgene blog. CRISPR Pooled Libraries Addgene's...

  3. The Pleiades Promoter Project

    Type
    Collection
    ...Promoter Project (Link opens in a new window) was established to advance research and therapies for brain disorders...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...ligand DKFZp686F2250, KL-1, Kitl, MGF, SCF, SF, SHEP7 KL klotho - LACRT lacritin MGC71934 LECT2 leukocyte... stimulating hormone receptor) MGC14337, MSH-R, SHEP2 MC2R melanocortin 2 receptor (adrenocorticotropic...Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. 2018. ImmPort...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...(s) online (left), choose to submit as published or unpublished. Fields marked with an asterisk are required...submitting plasmid(s) using a spreadsheet (right), click the “Download Deposit Spreadsheet” button to download ... the online form you can submit "Published plasmids" or "Unpublished or pre-publication plasmids" and ...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that...(for online submission) or "Download Deposit Spreadsheet" (for emailed submission) in the Deposit menu..."Submit Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid deposit There are two options...Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered using...

  8. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiSSTe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...

  9. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...deeper imaging into thick tissues. Light Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration...evanescent energy field. Lightsheet microscopes that project ultra-thin sheets of excitation light through...tissues need to be imaged via Lightsheet microscopy. Although not all lightsheet microscopes can cover samples...1); similar to an MRI or CT scanner. This is accomplished by focusing excitation light to a point in the... bleaching) to a single focal point. This is accomplished by using, for example, red light instead of ...configuration of two or more objectives to create a thin sheet of excitation light that propagates perpendicular...sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-shaped beams...

  10. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...sharing policy. Since early March, the open access publisher has required authors to include a note as to where...result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm...increasing the ability of reviewers (and, once published, anyone) to detect possible errors in assumptions...their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel tools and... video, generating huge amounts of data. When published, this data is usually condensed into a statistically... data are likely to improve research. What is published is typically a tiny fraction of what was actually...data accessibility and methodological detail. Publishers have also contributed to this issue by requiring...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...research project, the vast majority of what gets published in scientific journals flies under the radar and...have time to keep up with all the research being published on our own topic. Only on occasion do we then ...   Video abstracts about research, especially published research, have grown in popularity over the last...this: “In terms of total view count and videos published, YouTube reported a total of 865,995 views for...online article downloads – especially if they are published in open access journals.   Check Out Addgene Videos...Facebook is continuing its growth, and while many publishers and researchers have Facebook pages, few share... leading the way, the future of complementing published research with video overviews was growing quickly...

  12. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues...Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid...pAAV-mDlx-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 ...

  13. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...and an ever increasing number of journals and publishers to choose from, it gets very confusing for scientists...research may stipulate that your article must be published in an open access journal or by using the open...your manuscript will be available to anyone who wishes to read it, without them needing a subscription...data resulting from their funding should also be published. All these factors will influence your choice ...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...referee report are permanently attached to the published article. In addition, authors and readers are ...help your chances of getting your manuscripts published in the future. Disregard reviewer’s comments without...

  14. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...of the number of papers with your search term published each year, which is a nice way to see growth or...receive a daily, weekly, or monthly digest of newly published papers that contain that term. You can also set... a collection, which is essentially a virtual bookshelf. Pro tip The “cited by” and references sections...papers related to a particular topic or that were published by the lab down the hall.  Scientific journals...graph of the number of papers with your key term published each year is displayed with your search results...searches to particular authors, with the authors who published the most papers with your keyword populated at...Meta. Feeds automatically update as papers are published, similar to Facebook or Instagram. Key features...

  15. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ... hold from public access until the article is published. This policy gives us time to complete necessary...ready for distribution as soon as the paper is published, while giving you peace of mind that your materials... Emailing Addgene might not be as high on the published-paper-celebration checklist as champagne or cupcakes...become aware the related journal article has been published we’ll get the plasmids online for distribution...for you to notify us as soon as your article is published. As much as we’d like to, we can’t keep up with...the plasmids available as soon as your paper is published helps maximize the potential impact of your work...the plasmids useful, we’re happy to distribute unpublished materials. What about preprints? Addgene supports...

  16. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...like ReFigure which aims to consolidate published and unpublished experiments on similar subjects in a single...the abbreviated methods section found within a published manuscript. Luckily, many organizations have been...thereby enabling you to do modified analyses on published data if you need to. Similarly, FigShare also ...averse to producing papers that repeat previously published results. This over-reliance on the perfect story...problem is that only these positive results will be published. The publication record will then show that the...success story) even though the majority of the unpublished evidence shows otherwise. Others will go on to...very active with many scientists sharing their unpublished data. There are blogs - both hosted by individuals...

  17. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... but if available, use an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96...controller Microcentrifuge Microplate shaker ELISA plate washer (optional) Scale Microsoft Excel or similar software... but if available, use an automated ELISA plate washer. Seal the plate. Place the plate on a microplate... . Repeat steps 8–10 twice for a total of three washes. After the final wash, aspirate the wash buffer... but if available, use an automated ELISA plate washer. Seal the plate Place the plate on a microplate.... Repeat steps 18–20 twice for a total of three washes. After the final wash, aspirate the wash buffer...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...mitochondria or nucleus, will likely require a harsher lysis buffer than proteins easily available in ...in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, like RIPA, should be considered.... BME smells like rotting eggs but has a longer shelf life when stored properly. Because BME is not as... 1–2 hours Easy and cheap to handcast Short shelf-life; can alter proteins due to high pH Bis-...for proteins <40 kDa 180 V, 30 minutes Good shelf life; runs at neutral pH (reduced protein alteration...for proteins > 30 kDA 200 V, 30 minutes Good shelf life; runs at neutral pH (reduced protein alteration...

  19. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...the transfection in: 15 cm dishes OR CF5 stacks Select how many dishes or stacks you plan to transfect...for the type of tissue culture dish and number of dishes used. Here’s to making AAV packaging a little easier...1 to 10 Fill in experimental details on the spreadsheet: Name your viral prep Enter the specific names... plasmids Enter plasmid concentration The spreadsheet will calculate the following μg of each plasmid...with its corresponding column in the calculator spreadsheet: We hope that this calculator will make it easier...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut...mRNA cap, provides the most accurate mapping (Radzisheuskaya et al., 2016). In this case, location and sequence...nevertheless still lead to activity has been established, and can be used to identify sites that are likely...Zhivich V, Najgebauer H, Allen F, Gonçalves E, Shepherd R, Doench JG, Yusa K, Vazquez F, Parts L, Boehm...Boehm JS, Golub TR, Hahn WC, Root DE, Garnett MJ, Tsherniak A, Iorio F (2019) Agreement between two large .... https://doi.org/10.1038/s41587-020-0490-7 Radzisheuskaya A, Shlyueva D, Müller I, Helin K (2016) Optimizing...
Showing: 181 - 200 of 602 results