We narrowed to 267 results for: ttl

1. Experimenting with New Careers while in Grad School

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   ...few weeks into volunteering, I was talking to a little girl about fossils. After we talked for about ten...

2. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...reproductive confinement typically require very little effort to implement (depending on the species in...

3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...point?  Antibodies are large proteins that need a little help crossing cell membranes as they are not able...

4. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ... the effects of the antibiotic. This buys you a little more time, since bacteria won’t divide immediately...

5. Getting to Know Expansion Microscopy

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   ...doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging cellular ...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...set oneself. As a scientist who enjoys learning a little bit about many different things (part of the reason...

7. A Guide to Starting Your Own Journal Club

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   ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

8. Simplify Cloning with in vivo Assembly

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   ...biology project, yet very often, it is also a bottleneck in the experimental process. The majority of ...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...Cas9-gRNA RNPs show promise for in vivo studies, little is known about the immunogenicity and stability...

10. AAVs in Retinal Gene Therapy

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    ...started in the mid-90's with a lot of work and a little bit of luck A pioneer in the field, Dr. Jean Bennett...

11. Course-Based Undergraduate Research Experiences (CUREs)

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    ... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...

12. Transferable Skills Guide: Identifying Your Transferable Skills

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    ... pounding, you’re short of breath, and you’re a little jumpy. You are at another interview, and this job...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...import" technologies developed abroad, so it's a little boring. I think that such tendency is one of the...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...required to maintain fluorescent activity; very little truncation is tolerated, however, point mutations...

15. Twenty Years of Sharing: Expanding Accessibility

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    ...that persisted despite our best efforts. With a little over half of our orders coming from researchers...

16. Antibodies 101: Reading a Flow Plot

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    ...reported from flow cytometry experiments can be a little challenging to understand if you’ve never done ...

17. Viral Vectors 101: Preparing Pooled Libraries

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    ...pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such ...

18. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication syndrome or Rett...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...this approach has been proven feasible, it leaves little room for additional regulatory elements. Feng Zhang's...

20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...