We narrowed to 265 results for: ttl

1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...Cas9-gRNA RNPs show promise for in vivo studies, little is known about the immunogenicity and stability...

2. AAVs in Retinal Gene Therapy

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   Blog Post

   ...started in the mid-90's with a lot of work and a little bit of luck A pioneer in the field, Dr. Jean Bennett...

3. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ... pounding, you’re short of breath, and you’re a little jumpy. You are at another interview, and this job...

4. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   Blog Post

   ...set oneself. As a scientist who enjoys learning a little bit about many different things (part of the reason...

5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...point?  Antibodies are large proteins that need a little help crossing cell membranes as they are not able...

6. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ... the effects of the antibiotic. This buys you a little more time, since bacteria won’t divide immediately...

7. Getting to Know Expansion Microscopy

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   Blog Post

   ...doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging cellular ...

8. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...

9. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

10. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...biology project, yet very often, it is also a bottleneck in the experimental process. The majority of ...

11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...import" technologies developed abroad, so it's a little boring. I think that such tendency is one of the...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...required to maintain fluorescent activity; very little truncation is tolerated, however, point mutations...

13. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...scientific field. Reaching out to people who have little in common with you is much harder than starting...

14. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ... Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues...

15. How to Write a Scientific Review Article

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    Blog Post

    ...real time as I write. Things are going to get a little crazy (meaning you are probably going to cite hundreds...

16. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...experiment. Certain scientific communities have a little more leeway in what they can name a gene and many...

17. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...eating one before your chalk talk. Bring your own bottle of water. If possible, write down your short aims...

18. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication syndrome or Rett...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...this approach has been proven feasible, it leaves little room for additional regulatory elements. Feng Zhang's...

20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...