3D Printing Meets CRISPR Cas9
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...we love working with researchers on a model. The little knowledge that we have when we jump into a new ...
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Experimenting with New Careers while in Grad School
TypeBlog Post...few weeks into volunteering, I was talking to a little girl about fossils. After we talked for about ten... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...reproductive confinement typically require very little effort to implement (depending on the species in... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post... the effects of the antibiotic. This buys you a little more time, since bacteria won’t divide immediately... -
Getting to Know Expansion Microscopy
TypeBlog Post...doi.org/10.1016/j.neuron.2010.11.021 Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging cellular ... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...set oneself. As a scientist who enjoys learning a little bit about many different things (part of the reason... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...biology project, yet very often, it is also a bottleneck in the experimental process. The majority of ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...Cas9-gRNA RNPs show promise for in vivo studies, little is known about the immunogenicity and stability... -
AAVs in Retinal Gene Therapy
TypeBlog Post...started in the mid-90's with a lot of work and a little bit of luck A pioneer in the field, Dr. Jean Bennett... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post... pounding, you’re short of breath, and you’re a little jumpy. You are at another interview, and this job... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...point? Antibodies are large proteins that need a little help crossing cell membranes as they are not able... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...import" technologies developed abroad, so it's a little boring. I think that such tendency is one of the... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...required to maintain fluorescent activity; very little truncation is tolerated, however, point mutations... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...that persisted despite our best efforts. With a little over half of our orders coming from researchers... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...reported from flow cytometry experiments can be a little challenging to understand if you’ve never done ... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such ... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...partitioning (Schumacher, 2012). This concept is a little more complex than simply competing for replication...