Addgene

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Showing: 181 - 200 of 259 results

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Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...set oneself. As a scientist who enjoys learning a little bit about many different things (part of the reason...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...biology project, yet very often, it is also a bottleneck in the experimental process. The majority of ...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...Cas9-gRNA RNPs show promise for in vivo studies, little is known about the immunogenicity and stability...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...point? Antibodies are large proteins that need a little help crossing cell membranes as they are not able...
AAVs in Retinal Gene Therapy

Type

Blog Post

...started in the mid-90's with a lot of work and a little bit of luck A pioneer in the field, Dr. Jean Bennett...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Type

Blog Post

...import" technologies developed abroad, so it's a little boring. I think that such tendency is one of the...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...required to maintain fluorescent activity; very little truncation is tolerated, however, point mutations...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such ...
Transferable Skills Guide: External Collaborations

Type

Blog Post

...scientific field. Reaching out to people who have little in common with you is much harder than starting...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

... Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...that persisted despite our best efforts. With a little over half of our orders coming from researchers...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...partitioning (Schumacher, 2012). This concept is a little more complex than simply competing for replication...
An Inside Look into Addgene's Dev Team

Type

Blog Post

... I enjoy doing code reviews because it’s like a little peek into the mind of the developer and I get to...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...experiment. Certain scientific communities have a little more leeway in what they can name a gene and many...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...eating one before your chalk talk. Bring your own bottle of water. If possible, write down your short aims...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication syndrome or Rett...
Antibodies 101: Reading a Flow Plot

Type

Blog Post

...reported from flow cytometry experiments can be a little challenging to understand if you’ve never done ...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...this approach has been proven feasible, it leaves little room for additional regulatory elements. Feng Zhang's...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...

Showing: 181 - 200 of 259 results

Results per page:

Capturing a Moment with Marc Zimmer’s “The State of Science”

A Guide to Starting Your Own Journal Club

Simplify Cloning with in vivo Assembly

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

AAVs in Retinal Gene Therapy

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Viral Vectors 101: Preparing Pooled Libraries

Transferable Skills Guide: External Collaborations

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Twenty Years of Sharing: Expanding Accessibility

Plasmids 101: Plasmid Incompatibility

An Inside Look into Addgene's Dev Team

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Designing Your Chalk Talk for the Academic Job Interview

Rett Syndrome: A History of Research and Therapeutic Outlooks

Antibodies 101: Reading a Flow Plot

Overcoming the AAV Size Limitation for CRISPR Delivery

The PAM Requirement and Expanding CRISPR Beyond SpCas9

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