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  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...postdocs in the final ruling was, for a lot of us, frankly quite surprising. Consortia of higher education... changes if we wish to minimize the hurt. Many thanks to our guest blogger Gary McDowell! Gary S. McDowell...

  3. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ...support and to monitor user replies, but I can’t thank Joanne enough for all the work she put into this.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...

  4. The Challenges of Cell Culture

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    Blog Post
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ...transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which ...Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet,...

  6. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman!  Todd Waldman...

  7. March for Science

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    Blog Post
    ...staff. Many thanks to our guest blogger, Stephanie Hays. Stephanie would like to additionaly thank Isaac Plant...

  8. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product on...

  9. Site Directed Mutagenesis by PCR

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    Blog Post
    ...Also shown are the “B” restriction sites, which flank the targeted region. Upon PCR amplification, the...minor modifications to a plasmid template. Many thanks to our Guest Blogger Kristian Laursen! Kristian...

  10. Evolution of Lab Techniques

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    Blog Post
    ...Technique" in the comments section below! Many thanks to our guest blogger Krissy Lyon! Krissy Lyon is...32. PubMed PMID: 6176866. 3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative...

  11. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post
    ...Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

  12. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post
    ...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. ...

  13. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...cost-effective and efficient manner than ever before, thanks to the development of site-specific nucleases (...the pitfalls common to other approaches. Many thanks to our Guest Bloggers Amar Singh and Mathew Goodwin...

  14. Transferable Skills Guide: Teamwork

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    Blog Post
    ...usually the products of teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to ...Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but ...

  16. Finding Your Science Policy Path

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    Blog Post
    ...networking---and they’re more accessible than ever thanks to the virtual world that the pandemic has foisted.... Image used with permission from SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science...

  17. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post
    ...calculated p values from smallest to largest and ranking them (1 being the smallest value). It calculates...significance by finding the largest p value where its rank divided by the total number of comparisons performed...

  19. Typing CRISPR Systems

    Type
    Blog Post
    ...overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3. Cas3 functions...D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017...
Showing: 201 - 220 of 498 results