We narrowed to 399 results for: MUT

1. What's New in CRISPR - Spring 2019

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   Blog Post

   ...DNA strand. The lab then found gain-of-function mutations that allows it to be repurposed for double strand...

2. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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   ...infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral sequences...

3. What's New in CRISPR - December 2019

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   ...Single base editor that induces C-to-T and A-to-G mutations Nozomu Yachie’s lab recently developed a base ...

4. Modulate the Activity of 17 Signaling Pathways with One Kit!

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   ...pathways, they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes...

5. Six Spooky Science Stories and Halloween at Addgene

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   ...discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective...

6. New Norepinephrine Indicators: nLightG and nLightR

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   ...adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and nLightR uses...

7. What's New in CRISPR - September 2019

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   ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

8. Working 9-5: an Addgene Co-op

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   ...useful to me as some days it was not as easy to commute as others. I know some fear that remote work would...

9. 6 Steps to Submitting a Resume That Gets Seen

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   Blog Post

   ...manager on paper or as an email send directly from a mutual connection. If the organization is small, any person...

10. Build Your CRISPR Vocabulary

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    Blog Post

    ...other hand, viruses can evolve to evade CRISPR by mutating these sequences. Pro tip! Various labs have created...

11. Plasmids 101: Gene, enhancer, and promoter trapping

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    ...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...

12. Hot Plasmids - October 2022

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    ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

13. Hot Plasmids and Viral Preps - July 2021

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    Blog Post

    ...control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia...

14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    ... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to...

15. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    ...confirm the identity of the gene and check for any mutations. Once we identify the gene, we can easily annotate...

16. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post

    ... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

17. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post

    ...CRISPR/Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases...

18. New Viral Vectors - Winter 2025

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    ...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...

19. Antibodies 101: Epitope Tags

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    Blog Post

    ...Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of the same protein...

20. CRISPR Challenges: Standardization and Homology Directed Repair

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    ...Kwart, including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing...