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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 541 results for: SHI

Showing: 201 - 220 of 541 results

  1. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...launched the BioBuilder Educational Foundation in partnership with high school teachers to boost interest and...students include The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically...economically disadvantaged students for paid summer internships. A summer teacher professional development program...

  2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ... 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop took place in Boston...students, and we pay for travel expenses to our flagship workshop. In our view, the best possible outcome...is a co-founder of ComSciCon and Chair of its Leadership Team.  Nathan did his PhD in astronomy and works...

  5. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...?) As a result, I’d always end up stressed and rushing, no matter how hard I tried to build in extra time...especially on days when I was tired, stressed, or rushing. I would spend the time quietly running through... consider it this way: When you’re stressed or rushing, you’re much more likely to make a mistake. When...

  7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    .... PubMed Central PMCID: PMC4517063. 3. Mizushima, N., Yoshimori, T., & Levine, B. (2010). Methods in Mammalian...Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the...

  8. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory, or clinical...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. ...likelihood of creating a functional knock-out via frameshift and minimize off-target effects. The library ...Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles... et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura K, et al. Nat. Methods 2009. PubMed PMID: 19915560... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

  10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your technology...

  12. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...

  13. Design Tips for Prime Editing

    Type
    Blog Post
    ...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...

  14. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...: 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core ... 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected...vary depending on which company you choose. If publishing is important to you, make sure that your project...an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new...NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In contrast...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are ...Wang et. al., 2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin ...

  19. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...and Challenges CUREs inspire students to take ownership of their work and become more actively involved...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings...

  20. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...A260/230 ratios. Instead, phenol is more likely to shift the peak of your sample's absorption spectra from... RNA and DNA absorb maximally at 260 nm, so distinguishing between the two can be difficult by spectrophotometry...higher A260/280 and a lower A260/230 than DNA, so shifts in these values could hint at the presence of RNA...
Showing: 201 - 220 of 541 results